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Study · In vitro (cells) · 2004

3 results

Nuclear localisation of the G-actin sequestering peptide thymosin beta4.

Huff T, Rosorius O, Otto AM, Müller CS, Ballweber E, Hannappel E, Mannherz HG

Journal of cell science5 Oct 2004Cited by 70 (Europe PMC)

PMID15466884 (opens in a new tab)DOI10.1242/jcs.01404 (opens in a new tab)PepBankPUB-15466884
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study3

2 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Mechanism

    In intact cells, microinjected fluorescently labelled thymosin beta4 showed diffuse cytoplasmic staining and pronounced nuclear staining.

    In plain wordsIn cells, labelled thymosin beta4 injected into the cell was found in the cytoplasm and strongly in the nucleus.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    Not applicableIntracellular localisation of labelled thymosin beta4 after microinjection
    Study

    n: not reported in source

    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated · microinjectionAs given in this study.

    Source sentences

    Summary
    Here we show that after microinjection into intact cells, fluorescently labelled thymosin beta4 has a diffuse cytoplasmic and a pronounced nuclear staining.
    1 context sentence
    Summary · context
    It can be selectively labelled at two of its glutamine-residues with fluorescent Oregon Green cadaverine using transglutaminase; however, this labelling does not interfere with its interaction with G-actin.
  2. 02LabIn vitro (cells)Mechanism

    In digitonin-permeabilised and extracted cells, fluorescent thymosin beta4 stayed in the cytoplasm, and it concentrated in the nuclei after an additional Triton X100 extraction.

    In plain wordsIn permeabilised cells, labelled thymosin beta4 stayed in the cytoplasm, and after an extra extraction step it was found concentrated in the nuclei.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    Not applicableLocalisation of labelled thymosin beta4 in permeabilised cells
    Study

    n: not reported in source

    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    We further showed that in digitonin permeabilised and extracted cells, fluorescent thymosin beta4 was solely localised within the cytoplasm, whereas it was found concentrated within the cell nuclei after an additional Triton X100 extraction.
    1 context sentence
    Summary · context
    It can be selectively labelled at two of its glutamine-residues with fluorescent Oregon Green cadaverine using transglutaminase; however, this labelling does not interfere with its interaction with G-actin.

Studies that did not test the peptide alone1

In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Thymosin beta-4 (human).

  1. 03LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cells)Mechanism

    In cells, only the larger N-terminal fragment of labelled thymosin beta4, which holds the proposed actin-binding sequence, entered the nucleus. The smaller C-terminal fragment stayed in the cytoplasm.

    In plain wordsIn cells, only the larger N-terminal piece of thymosin beta4 entered the nucleus. The smaller C-terminal piece stayed in the cytoplasm.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    Not applicableNuclear localisation of labelled thymosin beta4 fragments
    Study

    n: not reported in source

    Outcome group
    Receptor activity and mechanism · Pharmacology

    Source sentences

    Summary
    Enzymatic cleavage of fluorescently labelled thymosin beta4 with AsnC-endoproteinase yielded two mono-labelled fragments of the peptide. After microinjection of these fragments, only the larger N-terminal fragment, containing the proposed actin-binding sequence exhibited nuclear localisation, wherea…
    1 context sentence
    Summary · context
    It can be selectively labelled at two of its glutamine-residues with fluorescent Oregon Green cadaverine using transglutaminase; however, this labelling does not interfere with its interaction with G-actin.

    In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 01:52 UTC

    Study results updated

  2. Version 1

    12 Oct 2026, 18:17 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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