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Study · In vitro (cells) · chinese hamster · 2004

5 results

N-Formylated humanin activates both formyl peptide receptor-like 1 and 2.

Harada M, Habata Y, Hosoya M, Nishi K, Fujii R, Kobayashi M, Hinuma S

Biochemical and biophysical research communications1 Nov 2004Cited by 89 (Europe PMC)

PMID15465011 (opens in a new tab)DOI10.1016/j.bbrc.2004.09.046 (opens in a new tab)PepBankPUB-15465011
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study5

4 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Activity measurement

    In CHO cells expressing human FPRL1 or FPRL2, humanin suppressed forskolin-induced cAMP production.

    In plain wordsIn cells carrying human receptors, humanin lowered cAMP production, which shows that it acts on these receptors.

    Peptide
    Humanin
    Outcome
    DecreaseForskolin-induced cAMP production
    Study

    Chinese hamstern: not reported in source

    CHO cells expressing human FPRL1 or FPRL2
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    HN suppressed forskolin-induced cAMP production in Chinese hamster ovary (CHO) cells expressing human FPRL1 (CHO-hFPRL1) or human FPRL2 (CHO-hFPRL2).
  2. 02LabIn vitro (cells)Activity measurement

    In CHO-hFPRL1 cells, the EC50 of humanin was 3.5nM.

    In plain wordsIn cells carrying the human FPRL1 receptor, humanin acted on the receptor at a half-maximal concentration of 3.5nM.

    Peptide
    Humanin
    Outcome
    Not applicableEC50 of humanin at FPRL1
    Study

    Chinese hamstern: not reported in source

    CHO cells expressing human FPRL1 or FPRL2
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Measurements

    EC50
    3.5 nM
    HN, CHO-hFPRL1 cells

    Source sentences

    Summary
    in CHO-hFPRL1 cells, the effective concentration for the half-maximal response (EC(50)) value of HN was 3.5nM
  3. 03LabIn vitro (cells)Mechanism

    In binding experiments, humanin and N-formylated humanin directly interacted with hFPRL1 on the cell membrane.

    In plain wordsIn lab tests, humanin and its formylated form bound directly to the human FPRL1 receptor on cell membranes.

    Peptide
    Humanin
    Outcome
    Not applicableBinding of humanin to hFPRL1
    Study

    Chinese hamstern: not reported in source

    CHO cells expressing human FPRL1 or FPRL2
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    We demonstrated by binding experiments using [(125)I]-W peptide that HN and fHN directly interacted with hFPRL1 on the membrane.
  4. 04LabIn vitro (cells)Activity measurement

    Humanin and N-formylated humanin showed strong chemotactic activity for CHO cells expressing hFPRL1 or hFPRL2.

    In plain wordsIn cells carrying human receptors, humanin and its formylated form strongly drew cells to move toward them.

    Peptide
    Humanin
    Outcome
    IncreaseChemotactic activity on CHO-hFPRL1 and CHO-hFPRL2 cells
    Study

    Chinese hamstern: not reported in source

    CHO cells expressing human FPRL1 or FPRL2
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    In addition, we found that HN and fHN showed strong chemotactic activity for CHO-hFPRL1 and CHO-hFPRL2 cells.

Studies that did not test the peptide alone1

In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Humanin.

  1. 05LabNot a test of Humanin aloneIn vitro (cells)Activity measurement

    In CHO-hFPRL1 cells, N-formylated humanin was more potent at FPRL1 than humanin, with an EC50 of 0.012nM versus 3.5nM.

    In plain wordsIn cells carrying the human FPRL1 receptor, formylated humanin worked at a much lower concentration (0.012nM) than plain humanin (3.5nM).

    Peptide
    Humanin
    Outcome
    Not applicableEC50 of N-formylated humanin at FPRL1
    Study

    Chinese hamstern: not reported in source

    CHO cells expressing human FPRL1 or FPRL2
    Outcome group
    Receptor activity and mechanism · Pharmacology

    Measurements

    EC50
    0.012 nM
    fHN, CHO-hFPRL1 cells

    Source sentences

    Summary
    N-formylated HN (fHN) performed more potently as a ligand for FPRL1 than HN: in CHO-hFPRL1 cells, the effective concentration for the half-maximal response (EC(50)) value of HN was 3.5nM, while that of fHN was 0.012nM

    In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Humanin.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 21:44 UTC

    Study results updated

  2. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

Publication types

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