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Study · Not a test of the peptide alone · 2004

4 results

Lanthanide-based time-resolved fluorescence of in cyto ligand-receptor interactions.

Handl HL, Vagner J, Yamamura HI, Hruby VJ, Gillies RJ

Analytical biochemistry1 Jul 2004Cited by 50 (Europe PMC)

PMID15203329 (opens in a new tab)DOI10.1016/j.ab.2004.04.012 (opens in a new tab)PepBankPUB-15203329
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study4

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone4

In this study, Melanotan I (afamelanotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Melanotan I (afamelanotide).

  1. 01LabNot a test of Melanotan I (afamelanotide) aloneIn vitro (cells)Activity measurement

    Eu-DTPA-labeled peptide ligands gave competitive binding results comparable to traditional radiolabeled binding assays.

    In plain wordsIn lab tests, peptides with a europium label gave binding results close to those of the older radioactive method.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    Not applicableCompetitive binding with Eu-DTPA-labeled ligands versus radiolabeled assay
    Study

    n: not reported in source

    Cells expressing melanocortin receptors
    Outcome group
    Receptor activity and mechanism · Pharmacology

    Source sentences

    Summary
    These labeled ligands were used in competitive binding assays with results comparable to those obtained using the traditional radiolabeled binding assays.
    1 context sentence
    Summary · context
    We verified binding of Eu-DTPA-NDP-alpha-MSH to cells overexpressing the human melanocortin-4 receptor.

    In this study, Melanotan I (afamelanotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Melanotan I (afamelanotide).

  2. 02LabNot a test of Melanotan I (afamelanotide) aloneIn vitro (cells)Activity measurement

    Time-resolved fluorescence detected attomoles of europium and allowed assays in 96-well plates, compared with 30-mm dishes for (125)I binding assays.

    In plain wordsIn lab tests, the europium method detected very small amounts and worked in 96-well plates instead of 30-mm dishes.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    Not applicableSensitivity and plate format of time-resolved fluorescence assay
    Study

    n: not reported in source

    Cells expressing melanocortin receptors
    Outcome group
    Receptor activity and mechanism · Pharmacology

    Source sentences

    Summary
    The sensitivity of time-resolved fluorescence is sufficient to detect attomoles of europium, allowing assays in 96-well plates, compared with 30-mm dishes for (125)I binding assays to whole cells.
    1 context sentence
    Summary · context
    We verified binding of Eu-DTPA-NDP-alpha-MSH to cells overexpressing the human melanocortin-4 receptor.

    In this study, Melanotan I (afamelanotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Melanotan I (afamelanotide).

  3. 03LabNot a test of Melanotan I (afamelanotide) aloneIn vitro (cells)Activity measurement

    Eu-labeled NDP-alpha-MSH bound cells overexpressing the human melanocortin-4 receptor with an affinity similar to that of unlabeled NDP-alpha-MSH.

    In plain wordsIn cells with the human MC4 receptor, the europium-labeled peptide bound about as tightly as the unlabeled peptide.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    Not applicableBinding affinity of Eu-DTPA-NDP-alpha-MSH versus unlabeled NDP-alpha-MSH
    Study

    n: not reported in source

    Cells expressing melanocortin receptors
    Comparator
    Unlabeled NDP-alpha-MSH
    Outcome group
    Receptor activity and mechanism · Pharmacology

    Source sentences

    Summary
    The Eu-labeled ligand bound to these cells with an affinity similar to that of unlabeled NDP-alpha-MSH and was used to optimize a competitive binding assay.
    1 context sentence
    Summary · context
    We verified binding of Eu-DTPA-NDP-alpha-MSH to cells overexpressing the human melanocortin-4 receptor.

    In this study, Melanotan I (afamelanotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Melanotan I (afamelanotide).

  4. 04LabNot a test of Melanotan I (afamelanotide) aloneIn vitro (cells)Activity measurement

    The lanthanide-based assays gave superior results with higher throughput and removed the need for radioactive waste disposal.

    In plain wordsIn lab tests, the europium assay had higher throughput than the radioactive method and needed no radioactive waste disposal.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    Not applicableThroughput and radioactive waste of lanthanide-based assay
    Study

    n: not reported in source

    Cells expressing melanocortin receptors
    Outcome group
    Receptor activity and mechanism · Pharmacology

    Source sentences

    Summary
    The lanthanide-based assays provided superior results with higher throughput and eliminated the need for radioactive waste disposal.
    1 context sentence
    Summary · context
    We verified binding of Eu-DTPA-NDP-alpha-MSH to cells overexpressing the human melanocortin-4 receptor.

    In this study, Melanotan I (afamelanotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Melanotan I (afamelanotide).

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 21:44 UTC

    Study results updated

  2. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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