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Study · Not a test of the peptide alone · 2004

6 results

Humanin antagonists: mutants that interfere with dimerization inhibit neuroprotection by Humanin.

Hashimoto Y, Terashita K, Niikura T, Yamagishi Y, Ishizaka M, Kanekura K, Chiba T, Yamada M, Kita Y, Aiso S, Matsuoka M, Nishimoto I

The European journal of neuroscience1 May 2004Cited by 18 (Europe PMC)

PMID15128389 (opens in a new tab)DOI10.1111/j.0953-816x.2004.03298.x (opens in a new tab)PepBankPUB-15128389
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study6

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone6

In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Humanin.

  1. 01LabNot a test of Humanin aloneIn vitro (cells)Mechanism

    In neuronal cells, the homodimerization-defective humanin mutants P3A, L12A, S14A and P19A blocked neuroprotection by humanin.

    In plain wordsIn cells, four altered humanin forms blocked the nerve cell protection that normal humanin gives.

    Peptide
    Humanin
    Outcome
    DecreaseNeuroprotection by HN with homodimerization-defective HN mutants
    Study

    n: not reported in source

    Outcome group
    Neuroprotection · Nervous system and cognition

    Source sentences

    Summary
    Homodimerization-defective mutants, P3A-, L12A-, S14A- and P19A-HN, specifically blocked neuroprotection by HN, but not by activity-dependent neurotrophic factor.
    1 context sentence
    Summary · context
    Here we examined whether any of these 'null' mutants could function as dominant-negative mutants.

    In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Humanin.

  2. 02LabNot a test of Humanin aloneIn vitro (cells)Mechanism

    In neuronal cells, the humanin mutants P3A, L12A, S14A and P19A did not block neuroprotection by activity-dependent neurotrophic factor.

    In plain wordsIn cells, the same four altered humanin forms did not block nerve cell protection by a different factor, ADNF.

    Peptide
    Humanin
    Outcome
    No significant changeNeuroprotection by ADNF with homodimerization-defective HN mutants
    Study

    n: not reported in source

    Outcome group
    Neuroprotection · Nervous system and cognition

    Source sentences

    Summary
    Homodimerization-defective mutants, P3A-, L12A-, S14A- and P19A-HN, specifically blocked neuroprotection by HN, but not by activity-dependent neurotrophic factor.
    1 context sentence
    Summary · context
    Here we examined whether any of these 'null' mutants could function as dominant-negative mutants.

    In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Humanin.

  3. 03LabNot a test of Humanin aloneIn vitro (cells)Mechanism

    L12A-HN and G5A/L12A-HN inhibited humanin homodimerization.

    In plain wordsIn cells, two altered humanin forms stopped humanin from pairing with itself.

    Peptide
    Humanin
    Outcome
    DecreaseHN homodimerization with L12A-HN and G5A/L12A-HN
    Study

    n: not reported in source

    Outcome group
    Neuroprotection · Nervous system and cognition

    Source sentences

    Summary
    While L12A-HN and G5A/L12A-HN actually inhibited HN homodimerization
    1 context sentence
    Summary · context
    Here we examined whether any of these 'null' mutants could function as dominant-negative mutants.

    In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Humanin.

  4. 04LabNot a test of Humanin aloneIn vitro (cells)Mechanism

    S7A/L12A-HN had no effect on humanin homodimerization.

    In plain wordsIn cells, a third altered humanin form did not stop humanin from pairing with itself.

    Peptide
    Humanin
    Outcome
    No significant changeHN homodimerization with S7A/L12A-HN
    Study

    n: not reported in source

    Outcome group
    Neuroprotection · Nervous system and cognition

    Source sentences

    Summary
    S7A/L12A-HN had no effect.

    In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Humanin.

  5. 05LabNot a test of Humanin aloneIn vitro (cells)Mechanism

    Insertion of the S7A mutation, which blocks homodimerization of humanin, abolished the antagonizing function of L12A-HN.

    In plain wordsIn cells, adding the S7A change removed the ability of altered L12A-HN to block normal humanin.

    Peptide
    Humanin
    Outcome
    DecreaseAntagonizing function of L12A-HN with S7A insertion
    Study

    n: not reported in source

    Outcome group
    Neuroprotection · Nervous system and cognition

    Source sentences

    Summary
    Furthermore, insertion of S7A, the mutation that blocks the homodimerization of HN, but not insertion of G5A abolished the antagonizing function of L12A-HN.
    1 context sentence
    Summary · context
    Here we examined whether any of these 'null' mutants could function as dominant-negative mutants.

    In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Humanin.

  6. 06LabNot a test of Humanin aloneIn vitro (cells)Mechanism

    Insertion of the G5A mutation did not abolish the antagonizing function of L12A-HN.

    In plain wordsIn cells, adding the G5A change did not remove the ability of altered L12A-HN to block normal humanin.

    Peptide
    Humanin
    Outcome
    No significant changeAntagonizing function of L12A-HN with G5A insertion
    Study

    n: not reported in source

    Outcome group
    Neuroprotection · Nervous system and cognition

    Source sentences

    Summary
    Furthermore, insertion of S7A, the mutation that blocks the homodimerization of HN, but not insertion of G5A abolished the antagonizing function of L12A-HN.
    1 context sentence
    Summary · context
    Here we examined whether any of these 'null' mutants could function as dominant-negative mutants.

    In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Humanin.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 21:44 UTC

    Study results updated

  2. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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