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Study · In vitro (cells) · 2004

2 results

Characterization of cell lines stably expressing human normal or mutated EGFP-tagged MC4R.

Blondet A, Doghman M, Rached M, Durand P, Bégeot M, Naville D

Journal of biochemistry1 Apr 2004Cited by 10 (Europe PMC)

PMID15115780 (opens in a new tab)DOI10.1093/jb/mvh064 (opens in a new tab)PepBankPUB-15115780
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study2

1 result from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Mechanism

    In HEK 293 cells stably expressing hMC4-R-EGFP, NDP-alpha MSH induced a time-dependent internalization of MC4-R.

    In plain wordsIn cells, NDP-alpha MSH made the MC4 receptor move into the cell over time.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    IncreaseMC4-R internalization
    Study

    n: not reported in source

    HEK 293 cells stably expressing hMC4-R-EGFP
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    NDP-alpha MSH induced a time-dependent internalization of MC4-R
    1 context sentence
    Summary · context
    After transfection in HEK 293, clones stably expressing hMC4-R-EGFP were selected.

Studies that did not test the peptide alone1

In this study, Melanotan I (afamelanotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Melanotan I (afamelanotide).

  1. 02LabNot a test of Melanotan I (afamelanotide) aloneIn vitro (cells)Mechanism

    In HEK 293 cells stably expressing hMC4-R-EGFP, AgRP partially prevented the internalization of MC4-R induced by NDP-alpha MSH.

    In plain wordsIn cells, AgRP partly stopped the MC4 receptor from moving inside after NDP-alpha MSH.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    DecreaseNDP-alpha MSH-induced internalization with AgRP
    Study

    n: not reported in source

    HEK 293 cells stably expressing hMC4-R-EGFP
    Outcome group
    Receptor activity and mechanism · Pharmacology

    Source sentences

    Summary
    NDP-alpha MSH induced a time-dependent internalization of MC4-R that was partially prevented by AgRP.
    1 context sentence
    Summary · context
    After transfection in HEK 293, clones stably expressing hMC4-R-EGFP were selected.

    In this study, Melanotan I (afamelanotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Melanotan I (afamelanotide).

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 21:44 UTC

    Study results updated

  2. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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