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Study · In vitro (cells) · 2004

6 results

Thymosin beta 4 stimulates laminin-5 production independent of TGF-beta.

Sosne G, Xu L, Prach L, Mrock LK, Kleinman HK, Letterio JJ, Hazlett LD, Kurpakus-Wheater M

Experimental cell research1 Feb 2004Cited by 56 (Europe PMC)

PMID14729067 (opens in a new tab)DOI10.1016/j.yexcr.2003.09.022 (opens in a new tab)PepBankPUB-14729067
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study6

4 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Mechanism

    In human corneal epithelial cells, Tbeta(4) increased laminin-5 gamma2 chain expression by more than 2-fold over untreated cells.

    In plain wordsIn human corneal cells in the lab, Tbeta(4) raised laminin-5 gamma2 chain expression more than 2-fold compared with untreated cells.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    IncreaseLaminin-5 gamma2 chain expression
    Study

    humann: not reported in source

    human corneal epithelial cells
    Comparator
    untreated cells
    Outcome group
    Wound healing · Eye
    Studied dose
    Dose not stated

    Measurements

    Fold increase
    > 2 fold
    LM-5 gamma2 chain versus untreated cells

    Source sentences

    Summary
    Using gene expression analysis with human corneal epithelial cells treated with Tbeta(4), we find that both LM-5 gamma2 chain and transforming growth factor beta 1 (TGFbeta-1) are increased by more than 2-fold over untreated cells.
  2. 02LabIn vitro (cells)Mechanism

    In human corneal epithelial cells, Tbeta(4) increased TGFbeta-1 expression by more than 2-fold over untreated cells.

    In plain wordsIn human corneal cells in the lab, Tbeta(4) raised TGFbeta-1 expression more than 2-fold compared with untreated cells.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    IncreaseTGFbeta-1 expression
    Study

    humann: not reported in source

    human corneal epithelial cells
    Comparator
    untreated cells
    Outcome group
    Wound healing · Eye
    Studied dose
    Dose not stated

    Measurements

    Fold increase
    > 2 fold
    TGFbeta-1 versus untreated cells

    Source sentences

    Summary
    Using gene expression analysis with human corneal epithelial cells treated with Tbeta(4), we find that both LM-5 gamma2 chain and transforming growth factor beta 1 (TGFbeta-1) are increased by more than 2-fold over untreated cells.
  3. 03LabIn vitro (cells)Mechanism

    TGFbeta-1 did not appear to be the mechanism by which Tbeta(4) acts on laminin-5 gamma2 chain synthesis.

    In plain wordsIn lab tests, Tbeta(4) did not appear to raise laminin-5 gamma2 chain through TGFbeta-1.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    Not applicableRole of TGFbeta-1 in Tbeta(4) action on laminin-5 gamma2 chain
    Study

    humann: not reported in source

    human corneal epithelial cells
    Outcome group
    Wound healing · Eye
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Although TGFbeta-1 increases LM-5 synthesis in a dose-dependent manner, it does not appear to be the mechanism by which Tbeta(4) acts on LM-5 gamma2 chain synthesis based on three independent experiments.
  4. 04LabIn vitro (cells)Mechanism

    Tbeta(4) increased laminin-5 gamma2 chain expression at 2 h and the effect peaked at 6 h, whereas TGFbeta-1 increased it only at 4 h and peaked at 8 h.

    In plain wordsIn lab tests, Tbeta(4) raised laminin-5 gamma2 chain expression at 2 hours and peaked at 6 hours. TGFbeta-1 acted later, at 4 and 8 hours.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    IncreaseTime course of laminin-5 gamma2 chain expression
    Study

    humann: not reported in source

    human corneal epithelial cells
    Outcome group
    Wound healing · Eye
    Studied dose
    Dose not stated

    Measurements

    Onset
    2 h
    Tbeta(4) LM-5 gamma2 chain
    Peak
    6 h
    Tbeta(4) LM-5 gamma2 chain

    Source sentences

    Summary
    In a time-course analysis, Tbeta(4) increases LM-5 gamma2 chain expression at 2 h and peaks at 6 h, while TGFbeta-1 increases LM-5 gamma2 chain expression only at 4 h and peaks at 8 h.

Studies that did not test the peptide alone2

In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Thymosin beta-4 (human).

  1. 05AnimalNot a test of Thymosin beta-4 (human) aloneAnimal study (in vivo)Mechanism

    In TGFbeta-1 knock-out mice given Tbeta(4), laminin-5 gamma2 chain expression was higher than in wild-type mice given Tbeta(4).

    In plain wordsIn mice lacking TGFbeta-1 that were given Tbeta(4), laminin-5 gamma2 chain expression was higher than in normal mice given Tbeta(4).

    Peptide
    Thymosin beta-4 (human)
    Outcome
    IncreaseLaminin-5 gamma2 chain expression in TGFbeta-1 knock-out mice
    Study

    mousen: not reported in source

    TGFbeta-1 knock-out mice
    Comparator
    wild-type mice treated with Tbeta(4)
    Outcome group
    Wound healing · Eye

    Source sentences

    Summary
    Finally, in TGFbeta-1 knock-out mice, Tbeta(4) increases LM-5 gamma2 chain expression to levels higher than that observed in wild-type mice treated with Tbeta(4).

    In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).

  2. 06LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cells)Mechanism

    Blocking TGFbeta-1 with neutralizing antibodies in Tbeta(4)-treated corneal epithelial cells increased laminin-5 gamma2 chain expression.

    In plain wordsIn lab tests, blocking TGFbeta-1 in Tbeta(4)-treated corneal cells increased laminin-5 gamma2 chain expression.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    IncreaseLaminin-5 gamma2 chain expression with TGFbeta-1 neutralizing antibodies
    Study

    humann: not reported in source

    human corneal epithelial cells
    Outcome group
    Wound healing · Eye

    Source sentences

    Summary
    When Tbeta(4)-induced LM-5 gamma2 chain expression is blocked with neutralizing antibodies to TGFbeta-1, LM-5 gamma2 chain expression is increased.

    In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 01:52 UTC

    Study results updated

  2. Version 1

    12 Oct 2026, 18:17 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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