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Study · Not a test of the peptide alone · 2003

5 results

Identification of essential amino acids in Humanin, a neuroprotective factor against Alzheimer's disease-relevant insults.

Yamagishi Y, Hashimoto Y, Niikura T, Nishimoto I

Peptides1 Apr 2003Cited by 92 (Europe PMC)

PMID12860203 (opens in a new tab)DOI10.1016/s0196-9781(03)00106-2 (opens in a new tab)PepBankPUB-12860203
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study5

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone5

In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Humanin.

  1. 01LabNot a test of Humanin aloneIn vitro (cells)Mechanism

    Intracellularly expressed HN-EGFP was secreted into the extracellular space, whereas neither EGFP nor (L9R)HN-EGFP was secreted.

    In plain wordsIn lab cells, tagged humanin was released from the cells, but the tag alone and a Leu9 mutant were not.

    Peptide
    Humanin
    Outcome
    Not applicableExtracellular secretion of HN-EGFP
    Study

    n: not reported in source

    cells expressing HN-EGFP and Ala-scanned HN constructs
    Outcome group
    Receptor activity and mechanism · Nervous system and cognition

    Source sentences

    Summary
    Intracellulary expressed HN-EGFP (EGFP N-terminally fused with HN) was extracellularly secreted, whereas neither EGFP nor (L9R)HN-EGFP was secreted at all.

    In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Humanin.

  2. 02LabNot a test of Humanin aloneIn vitro (cells)Mechanism

    Arg substitution showed that the Leu9-Leu11 structure and a second structure around Pro19 were essential for secretion of full-length humanin, while Ala substitutions did not affect secretion.

    In plain wordsIn lab cells, two short regions of humanin were needed for its release from cells.

    Peptide
    Humanin
    Outcome
    Not applicableStructures essential for secretion of full-length HN
    Study

    n: not reported in source

    cells expressing HN-EGFP and Ala-scanned HN constructs
    Outcome group
    Receptor activity and mechanism · Nervous system and cognition

    Source sentences

    Summary
    While Ala substitution of neither residue affected HN secretion, Arg substitution revealed that the two structures-Leu9-Leu11 and Pro19-Va120-were essential for the secretion of full-length HN.

    In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Humanin.

  3. 03LabNot a test of Humanin aloneIn vitro (cells)Mechanism

    Asp substitution of Leu10, but not of Leu9 or Leu11, nullified the secretion of humanin.

    In plain wordsIn lab cells, changing one amino acid, number 10, stopped humanin from being released.

    Peptide
    Humanin
    Outcome
    Not applicableRole of Leu10 in HN secretion
    Study

    n: not reported in source

    cells expressing HN-EGFP and Ala-scanned HN constructs
    Outcome group
    Receptor activity and mechanism · Nervous system and cognition

    Source sentences

    Summary
    In the Leu9-Leu11 domain, the Leu10 residue turned out to play a central role in this function, because the Asp substitution of Leu10, but not Leu9 or Leu11, nullified the secretion of HN.

    In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Humanin.

  4. 04LabNot a test of Humanin aloneIn vitro (cells)Activity measurement

    In Ala-scanned humanin constructs, Pro3, Ser7, Cys8, Leu9, Leu12, Thr13, Ser14 and Pro19 were essential for the neuroprotective function of full-length humanin.

    In plain wordsIn lab cells, eight amino acids of humanin were needed for its protective function.

    Peptide
    Humanin
    Outcome
    Not applicableResidues essential for the neuroprotective function of HN
    Study

    n: not reported in source

    cells expressing HN-EGFP and Ala-scanned HN constructs
    Outcome group
    Neuroprotection · Nervous system and cognition

    Source sentences

    Summary
    Utilizing Ala-scanned HN constructs, we also investigated a comprehensive structure-function relationship for the neuroprotective function of full-length HN, which revealed (i) that Pro3, Ser7, Cys8, Leu9, Leu12, Thr13, Ser14, and Pro19 were essential for this function

    In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Humanin.

  5. 05LabNot a test of Humanin aloneIn vitro (cells)Mechanism

    Ser7 and Leu9 were essential for self-dimerization of humanin.

    In plain wordsIn lab cells, two amino acids of humanin were needed for it to pair with itself.

    Peptide
    Humanin
    Outcome
    Not applicableResidues essential for self-dimerization of HN
    Study

    n: not reported in source

    cells expressing HN-EGFP and Ala-scanned HN constructs
    Outcome group
    Receptor activity and mechanism · Nervous system and cognition

    Source sentences

    Summary
    (ii) that Ser7 and Leu9 were essential for self-dimerization of HN.

    In this study, Humanin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Humanin.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 21:44 UTC

    Study results updated

  2. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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