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Study · In vitro (cells) · 2003

1 result

Stable expression of human melanocortin 3 receptor fused to EGFP in the HEK293 cells.

Rached M, Buronfosse A, Durand P, Begeot M, Penhoat A

Biochemical and biophysical research communications1 Jun 2003Cited by 7 (Europe PMC)

PMID12788089 (opens in a new tab)DOI10.1016/s0006-291x(03)00934-3 (opens in a new tab)PepBankPUB-12788089
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study1

1 result from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Activity measurement

    In HEK293 clones stably expressing hMC3R-EGFP, NDP-MSH stimulated cAMP production in a dose-dependent manner with an EC50 of 0.3 nM.

    In plain wordsIn lab cells carrying the tagged MC3 receptor, NDP-MSH raised cAMP in a dose-dependent way.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    IncreasecAMP production in hMC3R-EGFP cells
    Study

    n: not reported in source

    HEK293 clones stably expressing hMC3R-EGFP
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Measurements

    EC50
    0.3 nM
    hMC3R-EGFP HEK293 clones

    Source sentences

    Summary
    responded to NDP-MSH stimulation by producing cAMP in a dose-dependent manner (EC(50): 0.3 nM)
    1 context sentence
    Summary · context
    The corresponding chimeric cDNA was stably expressed in HEK293 cells.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 21:44 UTC

    Study results updated

  2. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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