The binding IC50 values of radiolabeled hAGRP(86-132) and NDP-MSH were identical within experimental error.
In plain wordsIn lab tests, NDP-MSH and the AGRP fragment bound the receptor with the same strength, within experimental error.
- Peptide
- Melanotan I (afamelanotide)
- Outcome
- No significant changeBinding IC50 of radiolabeled hAGRP(86-132) versus NDP-MSH
- Study
n: not reported in source
Cells expressing MC3R and MC4R- Comparator
- Radiolabeled hAGRP(86-132)
- Outcome group
- Receptor activity and mechanism · Pharmacology
Source sentences
The binding IC(50) values of radiolabeled hAGRP(86-132) versus NDP-MSH were identical within experimental error, supporting the hypothesis that AGRP and NDP-MSH interact with overlapping binding epitopes at the MC3R and MC4R.
1 context sentence(design, population, supporting numbers)
For binding studies, radiolabeled 125I-NDP-MSH versus 125I-hAGRP(86-132) were utilized to determine if there were differences in the ability of the AGRP fragments prepared herein to competitively displace the 125I-NDP-MSH versus AGRP(86-132) radiolabel.
In this study, Melanotan I (afamelanotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Melanotan I (afamelanotide).