Thymosin beta4 regulation, expression and function in aortic valve interstitial cells.

Li QY, Jones PL, Lafferty RP, Safer D, Levy RJ

The Journal of heart valve disease1 Sept 2002Cited by 12 (Europe PMC)

PMID12358412PepBankPUB-12358412

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study6

2 results from studies that gave the peptide. No result from this study comes from people.

01LabIn vitro (cells)Mechanism
In sheep aortic valve interstitial cell cultures, added thymosin beta4 re-oriented cytoskeletal F-actin.
In plain wordsIn sheep valve cells, adding thymosin beta4 changed how the cells' actin fibres were arranged.
Peptide
Thymosin beta-4 (human)
Outcome
Not applicableCytoskeletal F-actin orientation
Study
sheepn: not reported in source
sheep aortic valve interstitial cells (SAVIC)
Outcome group
Receptor activity and mechanism · Cardiovascular
Studied dose
Dose not stated
Source sentences
Summary
The addition of thymosin beta4 to aortic valve interstitial cell cultures resulted in a re-orientation of cytoskeletal F-actin, and induction of MMP-2.
1 context sentence(design, population, supporting numbers)
Summary · context
Subtractive hybridization was performed using sheep aortic valve interstitial cells (SAVIC) grown on a substrate of collagen plus tenascin-C, versus cells grown on type I collagen alone.
Report a problemFND-1235841204
02LabIn vitro (cells)Mechanism
In sheep aortic valve interstitial cell cultures, added thymosin beta4 induced MMP-2.
In plain wordsIn sheep valve cells, adding thymosin beta4 raised MMP-2, an enzyme that breaks down tissue matrix.
Peptide
Thymosin beta-4 (human)
Outcome
IncreaseMMP-2 induction
Study
sheepn: not reported in source
sheep aortic valve interstitial cells (SAVIC)
Outcome group
Receptor activity and mechanism · Cardiovascular
Studied dose
Dose not stated
Source sentences
Summary
The addition of thymosin beta4 to aortic valve interstitial cell cultures resulted in a re-orientation of cytoskeletal F-actin, and induction of MMP-2.
1 context sentence(design, population, supporting numbers)
Summary · context
Subtractive hybridization was performed using sheep aortic valve interstitial cells (SAVIC) grown on a substrate of collagen plus tenascin-C, versus cells grown on type I collagen alone.
Report a problemFND-1235841205

Natural levels measured3

These results measure the body's own peptide. Nobody was given the peptide. They are not evidence about taking it. They are left out of key results, evidence counts and charts.

03LabEndogenous levelIn vitro (cells)Mechanism
In sheep aortic valve interstitial cells grown on tenascin-C, nearly 70% of isolated clones in the subtractive hybridization contained the thymosin beta‑4 sequence.
In plain wordsIn sheep valve cells, the cells' own thymosin beta‑4 gene made up most clones found when cells grew on tenascin-C.
Peptide
Thymosin beta-4 (human)
Outcome
IncreaseThymosin beta-4 clones in tenascin-C subtractive library
Study
sheepn: not reported in source
sheep aortic valve interstitial cells (SAVIC)
Comparator
Cells on type I collagen alone
Outcome group
Receptor activity and mechanism · Cardiovascular
Measurements
Clones containing thymosin beta-4 sequence
≈ 70%
subtractive hybridization
Source sentences
Summary
Subtractive hybridizations revealed that nearly 70% of the clones isolated contained the sequence for thymosin beta-4, an actin-binding protein, also associated with mineralization, regulation of MMPs and inflammation.
1 context sentence(design, population, supporting numbers)
Summary · context
Subtractive hybridization was performed using sheep aortic valve interstitial cells (SAVIC) grown on a substrate of collagen plus tenascin-C, versus cells grown on type I collagen alone.
This result measures the body's own Thymosin beta-4 (human). Nobody was given the peptide. It is not evidence about taking it.
Report a problemFND-1235841201
04LabEndogenous levelIn vitro (cells)Mechanism
Sheep aortic valve interstitial cells in culture produced both thymosin beta4 and thymosin beta4 sulfoxide.
In plain wordsIn sheep valve cells, the cells made their own thymosin beta4 and its oxidized form.
Peptide
Thymosin beta-4 (human)
Outcome
Not applicableProduction of thymosin beta4 and thymosin beta4 sulfoxide
Study
sheepn: not reported in source
sheep aortic valve interstitial cells (SAVIC)
Outcome group
Receptor activity and mechanism · Cardiovascular
Source sentences
Summary
In cell culture studies, it was shown that both thymosin beta4 and thymosin beta4 sulfoxide are produced by SAVIC.
This result measures the body's own Thymosin beta-4 (human). Nobody was given the peptide. It is not evidence about taking it.
Report a problemFND-1235841202
05LabEndogenous levelIn vitro (cells)Mechanism
In sheep aortic valve interstitial cells, growth on a tenascin-C substrate up-regulated thymosin beta4 expression.
In plain wordsIn sheep valve cells, growing on tenascin-C raised the cells' own thymosin beta4 expression.
Peptide
Thymosin beta-4 (human)
Outcome
IncreaseThymosin beta4 expression on tenascin-C substrate
Study
sheepn: not reported in source
sheep aortic valve interstitial cells (SAVIC)
Comparator
Cells on type I collagen alone
Outcome group
Receptor activity and mechanism · Cardiovascular
Source sentences
Summary
When aortic valve interstitial cells were grown on a substrate of tenascin-C, thymosin beta4 expression was up-regulated.
1 context sentence(design, population, supporting numbers)
Summary · context
Subtractive hybridization was performed using sheep aortic valve interstitial cells (SAVIC) grown on a substrate of collagen plus tenascin-C, versus cells grown on type I collagen alone.
This result measures the body's own Thymosin beta-4 (human). Nobody was given the peptide. It is not evidence about taking it.
Report a problemFND-1235841203

Studies that did not test the peptide alone1

In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Thymosin beta-4 (human).

06LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cells)Mechanism
In sheep aortic valve interstitial cells, thymosin beta4 antisense oligonucleotide transfection did not suppress MMP-2 expression.
In plain wordsIn sheep valve cells, blocking thymosin beta4 production with an antisense molecule did not lower MMP-2 expression.
Peptide
Thymosin beta-4 (human)
Outcome
No significant changeMMP-2 expression after thymosin beta4 antisense transfection
Study
sheepn: not reported in source
sheep aortic valve interstitial cells (SAVIC)
Outcome group
Receptor activity and mechanism · Cardiovascular
Source sentences
Summary
However, thymosin beta4 antisense oligonucleotide transfection did not suppress MMP-2 expression.
1 context sentence(design, population, supporting numbers)
Summary · context
Subtractive hybridization was performed using sheep aortic valve interstitial cells (SAVIC) grown on a substrate of collagen plus tenascin-C, versus cells grown on type I collagen alone.
In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).
Report a problemFND-1235841206

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
13 Oct 2026, 01:52 UTC
Study results updated
Version 1
12 Oct 2026, 18:17 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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