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Study · In vitro (cells) · 2001

2 results

Expression of functional melanocortin-4 receptor in the hypothalamic GT1-1 cell line.

Khong K, Kurtz SE, Sykes RL, Cone RD

Neuroendocrinology1 Sept 2001Cited by 47 (Europe PMC)

PMID11528221 (opens in a new tab)DOI10.1159/000054686 (opens in a new tab)PepBankPUB-11528221
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study2

2 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Activity measurement

    In hypothalamic GT1-1 and GT1-7 cells, NDP-alpha melanocyte-stimulating hormone showed high-affinity binding sites (Ki 1.1 x 10(-10) and 1.8 x 10(-10) M).

    In plain wordsIn cells from the hypothalamus, NDP-MSH bound to its receptor sites very tightly.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    Not applicableNDP-MSH binding affinity (Ki)
    Study

    n: not reported in source

    hypothalamic GT1-1 and GT1-7 cell lines
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones
    Studied dose
    Dose not stated

    Measurements

    Ki
    1.1 x 10(-10) M
    GT1-1 cells
    Ki
    1.8 x 10(-10) M
    GT1-7 cells

    Source sentences

    Summary
    High-affinity binding sites were demonstrated in the GT1-1 and GT1-7 cells for NDP-alpha melanocyte-stimulating hormone (MSH; K(i) = 1.1 x 10(-10) and 1.8 x 10(-10) M)
    1 context sentence
    Summary · context
    We report here a neuronal cell line that exhibits endogenous functional expression for the MC4-R.
  2. 02LabIn vitro (cells)Activity measurement

    In hypothalamic GT1-1 cells, NDP-alpha-MSH at 1 nM to 1 microM stimulated gonadotropin-releasing hormone (GnRH) secretion.

    In plain wordsIn hypothalamic cells in the lab, NDP-MSH made the cells release GnRH, a reproductive hormone.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    IncreaseGnRH secretion
    Study

    n: not reported in source

    hypothalamic GT1-1 and GT1-7 cell lines
    Comparator
    AGRP (no GnRH secretion)
    Outcome group
    Sex hormones and fertility · Endocrine and hormones
    Studied dose
    1 nM to 1 microMAs given in this study.

    Source sentences

    Summary
    Stimulation of gonadotropin-releasing hormone (GnRH) secretion was achieved with 1 nM to 1 microM concentrations of NDP-alpha-MSH while no GnRH secretion was observed when the GT1-1 cells were treated with AGRP.
    1 context sentence
    Summary · context
    We report here a neuronal cell line that exhibits endogenous functional expression for the MC4-R.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 21:44 UTC

    Study results updated

  2. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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