Skip to content

Study · Not a test of the peptide alone · 2001

3 results

Fluorescence study of conformational properties of melanotropins labeled with aminobenzoic acid.

Ito AS, Souza ES, dos Reis Barbosa S, Nakaie CR

Biophysical journal1 Aug 2001Cited by 11 (Europe PMC)

PMID11463659 (opens in a new tab)PMCIDPMC1301587 (opens in a new tab)DOI10.1016/s0006-3495(01)75775-x (opens in a new tab)PepBankPUB-11463659
Source linkPubMedAsk about this study
Jump to 3 results

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study3

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone3

In this study, Melanotan I (afamelanotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Melanotan I (afamelanotide).

  1. 01LabNot a test of Melanotan I (afamelanotide) aloneIn vitro (cell-free)Mechanism

    In solution, energy transfer occurred between tryptophan(9) and the Abz label, more strongly to the ortho form of the acceptor.

    In plain wordsIn lab tests, the fluorescent label and a tryptophan in the peptide exchanged energy, more with the ortho form of the label.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    Not applicableEnergy transfer between Trp(9) and Abz
    Study

    n: not reported in source

    peptides in solution (water, trifluoroethanol)
    Outcome group
    Peptide structure and chemistry · Pharmacology

    Source sentences

    Summary
    We observed energy transfer between the tryptophan(9) residue acting as donor and Abz as acceptor, the transfer being more pronounced to the ortho-form of the acceptor.
    1 context sentence
    Summary · context
    The native hormone alpha-melanocyte-stimulating hormone (alpha-MSH) and its more potent analog [Nle(4),D-Phe(7)]alpha-MSH (NDP-alpha MSH), labeled at the amino terminal with the fluorescent aminobenzoic acid (Abz) isomers, were examined by fluorescence methods.

    In this study, Melanotan I (afamelanotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Melanotan I (afamelanotide).

  2. 02LabNot a test of Melanotan I (afamelanotide) aloneIn vitro (cell-free)Mechanism

    In water, labeled NDP-alpha MSH showed three Abz-Trp(9) distance populations, indicating distinct conformational states compared with the native hormone.

    In plain wordsIn lab tests, labeled NDP-alpha MSH in water took several distinct shapes, unlike the native hormone.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    Not applicableAbz-Trp(9) distance populations of labeled NDP-alpha MSH in water
    Study

    n: not reported in source

    peptides in solution (water, trifluoroethanol)
    Outcome group
    Peptide structure and chemistry · Pharmacology

    Source sentences

    Summary
    Three different distance populations could be identified in the labeled analog NDP-alpha MSH in water, indicating distinct conformational states for the synthetic peptide, compared with the native hormone.
    1 context sentence
    Summary · context
    The native hormone alpha-melanocyte-stimulating hormone (alpha-MSH) and its more potent analog [Nle(4),D-Phe(7)]alpha-MSH (NDP-alpha MSH), labeled at the amino terminal with the fluorescent aminobenzoic acid (Abz) isomers, were examined by fluorescence methods.

    In this study, Melanotan I (afamelanotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Melanotan I (afamelanotide).

  3. 03LabNot a test of Melanotan I (afamelanotide) aloneIn vitro (cell-free)Mechanism

    In trifluoroethanol, two Abz-Trp(9) distance populations were recovered for both the native hormone and the labeled analog, reflecting fewer conformational states.

    In plain wordsIn lab tests, in trifluoroethanol both the native hormone and the analog took fewer shapes.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    Not applicableAbz-Trp(9) distance populations in trifluoroethanol
    Study

    n: not reported in source

    peptides in solution (water, trifluoroethanol)
    Outcome group
    Peptide structure and chemistry · Pharmacology

    Source sentences

    Summary
    Measurements in trifluoroethanol resulted in the recovery of two Abz-Trp(9) distance populations, both for the native and the analog hormones, reflecting the decrease, induced by the solvent, of the conformational states available to the peptides.
    1 context sentence
    Summary · context
    The native hormone alpha-melanocyte-stimulating hormone (alpha-MSH) and its more potent analog [Nle(4),D-Phe(7)]alpha-MSH (NDP-alpha MSH), labeled at the amino terminal with the fluorescent aminobenzoic acid (Abz) isomers, were examined by fluorescence methods.

    In this study, Melanotan I (afamelanotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Melanotan I (afamelanotide).

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 21:44 UTC

    Study results updated

  2. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

PepBank is a research reference. Talk to a doctor before you use any peptide.