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Study · In vitro (cells) · fish · 2001

6 results

Local photorelease of caged thymosin beta4 in locomoting keratocytes causes cell turning.

Roy P, Rajfur Z, Jones D, Marriott G, Loew L, Jacobson K

The Journal of cell biology1 May 2001Cited by 60 (Europe PMC)

PMID11381088 (opens in a new tab)PMCIDPMC2174335 (opens in a new tab)DOI10.1083/jcb.153.5.1035 (opens in a new tab)PepBankPUB-11381088
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: CC BY-NC. Results still quote short attributed sentences.

Results from this study6

6 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Mechanism

    In locomoting fish keratocytes, local photoactivation of caged Tbeta4 at the wings caused specific turning about the irradiated region, whereas various controls were negative.

    In plain wordsIn fish cells, light-activating Tbeta4 at one spot made the cells turn about that spot. Controls did not.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    IncreaseKeratocyte turning after local photoactivation of caged Tbeta4
    Study

    fishn: not reported in source

    Locomoting fish scale keratocytes
    Comparator
    Various controls
    Outcome group
    Receptor activity and mechanism · Whole body
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Indeed, when caged Tbeta4 was locally photoactivated at the wings of locomoting keratocytes, specific turning about the irradiated region was observed, whereas various controls were negative.
    1 context sentence
    Summary · context
    Specifically, a caged form of thymosin beta4 (Tbeta4) was photoactivated in a defined local region of locomoting fish scale keratocytes and the resulting perturbation of locomotion was studied.
  2. 02LabIn vitro (cells)Mechanism

    Loading exogenous Tbeta4 into keratocytes and fibroblasts caused very rapid disassembly of actin filaments.

    In plain wordsIn cells, loading in Tbeta4 quickly broke down actin filaments.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    IncreaseActin filament disassembly after loading exogenous Tbeta4
    Study

    n: not reported in source

    Keratocytes and fibroblasts loaded with Tbeta4
    Outcome group
    Receptor activity and mechanism · Whole body
    Studied dose
    Dose not stated

    Source sentences

    Summary
    loading of exogenous Tbeta4 into both keratocytes and fibroblasts caused very rapid disassembly of actin filaments and reduction of cellular contractility.
  3. 03LabIn vitro (cells)Mechanism

    Loading exogenous Tbeta4 into keratocytes and fibroblasts caused reduction of cellular contractility.

    In plain wordsIn cells, loading in Tbeta4 reduced how strongly the cells contract.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    DecreaseCellular contractility after loading exogenous Tbeta4
    Study

    n: not reported in source

    Keratocytes and fibroblasts loaded with Tbeta4
    Outcome group
    Receptor activity and mechanism · Whole body
    Studied dose
    Dose not stated

    Source sentences

    Summary
    loading of exogenous Tbeta4 into both keratocytes and fibroblasts caused very rapid disassembly of actin filaments and reduction of cellular contractility.
  4. 04LabIn vitro (cell-free)Mechanism

    In an in vitro assay, caged Tbeta4 did not change normal actin polymerization kinetics.

    In plain wordsIn lab tests, caged Tbeta4 did not change how actin builds up.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    No significant changeActin polymerization with caged Tbeta4
    Study

    n: not reported in source

    In vitro actin polymerization assay
    Comparator
    Normal actin polymerization kinetics
    Outcome group
    Receptor activity and mechanism · Whole body
    Studied dose
    Dose not stated

    Source sentences

    Summary
    In vitro spectrophotofluorometric assays indicated that caged Tbeta4 did not change the normal actin polymerization kinetics, whereas photoactivated Tbeta4 significantly inhibited actin polymerization.
  5. 05LabIn vitro (cell-free)Mechanism

    In an in vitro assay, photoactivated Tbeta4 significantly inhibited actin polymerization.

    In plain wordsIn lab tests, light-activated Tbeta4 strongly slowed actin build-up.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    DecreaseActin polymerization with photoactivated Tbeta4
    Study

    n: not reported in source

    In vitro actin polymerization assay
    Comparator
    Caged Tbeta4
    Outcome group
    Receptor activity and mechanism · Whole body
    Studied dose
    Dose not stated

    Source sentences

    Summary
    In vitro spectrophotofluorometric assays indicated that caged Tbeta4 did not change the normal actin polymerization kinetics, whereas photoactivated Tbeta4 significantly inhibited actin polymerization.
  6. 06Computer modelIn silicoMechanism

    Simulations predicted that local photoactivation of Tbeta4 in keratocytes could potentially elicit a regional locomotory response.

    In plain wordsIn computer simulations, local light activation of Tbeta4 in cells was predicted to possibly trigger a regional movement response.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    Not applicablePredicted regional locomotory response to local Tbeta4 photoactivation
    Study

    n: not reported in source

    Virtual Cell simulation
    Outcome group
    Receptor activity and mechanism · Whole body
    Studied dose
    Dose not stated

    Source sentences

    Summary
    These simulations demonstrated that locally photoactivating Tbeta4 in keratocytes could potentially elicit a regional locomotory response.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 01:52 UTC

    Study results updated

  2. Version 1

    12 Oct 2026, 18:17 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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