In HEK 293T cells, NDP-MSH was the most efficient alpha-MSH analog for CRE-mediated gene transcription at MC3R.
In plain wordsIn cells, NDP-MSH was the most effective of the tested alpha-MSH analogs at switching on the MC3R receptor.
- Peptide
- Melanotan I (afamelanotide)
- Outcome
- Not applicableCRE-mediated gene transcription at MC3R
- Study
humann: not reported in source
HEK 293T cells expressing MC3R or MC4R- Outcome group
- Receptor activity and mechanism · Pharmacology
- Studied dose
- Dose not stated
Source sentences
In our assay of CRE-mediated gene transcription activity, alpha-MSH-ND was the most efficient alpha-MSH analog for MC4R whereas NDP-MSH was the most efficient for MC3R.
1 context sentence(design, population, supporting numbers)
In this study we describe the functional response of melanocortin 4 receptor (MC4R) and melanocortin 3 receptor (MC3R) in HEK 293T cells, by using a luciferase reporter gene under the transcriptional control of a cAMP-responsive element (CRE) as a monitor of intracellular cAMP levels and cAMP-regulated gene expression.