A comparison of blood agar supplemented with NAD with plain blood agar and chocolated blood agar in the isolation of Streptococcus pneumoniae and Haemophilus influenzae from sputum. Bacterial Methods Evaluation Group.

Nye KJ, Fallon D, Gee B, Messer S, Warren RE, Andrews N

Journal of medical microbiology1 Dec 1999Cited by 7 (Europe PMC)

PMID10591166DOI10.1099/00222615-48-12-1111PepBankPUB-10591166

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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study4

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone4

In this study, NAD+ (nicotinamide adenine dinucleotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking NAD+ (nicotinamide adenine dinucleotide).

01LabNot a test of NAD+ (nicotinamide adenine dinucleotide) aloneIn vitro (cells)Activity measurement
In sputum cultures, NAD-supplemented blood agar isolated H. influenzae less often than plain plus chocolated blood agar.
In plain wordsIn lab tests, NAD-supplemented blood agar detected H. influenzae less often than the two-plate combination.
Peptide
NAD+ (nicotinamide adenine dinucleotide)
Outcome
DecreaseH. influenzae isolation versus two-plate combination
Study
Haemophilus influenzae; Streptococcus pneumoniaen = 1,724
Sputum culture on NAD-supplemented blood agar
Comparator
Plain blood agar plus chocolated blood agar
Outcome group
Infection and antimicrobial activity · Infection
Measurements
Isolation rate
8.16%
NAD-supplemented blood agar
Isolation rate
11.07%
two-plate combination
Source sentences
Summary
The two-plate combination performed significantly better for both organisms; isolation rates for H. influenzae were increased from 8.16% on SBA to 11.07% on BA plus CBA and for S. pneumoniae from 4.18% to 4.68%.
2 context sentences(design, population, supporting numbers)
Summary · context
In the present study, 1724 sputum samples were examined in four laboratories.
Summary · context
The isolation rates of H. influenzae and S. pneumoniae on NAD-supplemented blood agar (SBA) were compared with those on a two-plate combination of plain blood (BA) and chocolated blood agar (CBA).
In this study, NAD+ (nicotinamide adenine dinucleotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking NAD+ (nicotinamide adenine dinucleotide).
Report a problemFND-1059116601
02LabNot a test of NAD+ (nicotinamide adenine dinucleotide) aloneIn vitro (cells)Activity measurement
In sputum cultures, NAD-supplemented blood agar isolated S. pneumoniae less often than plain plus chocolated blood agar.
In plain wordsIn lab tests, NAD-supplemented blood agar detected S. pneumoniae less often than the two-plate combination.
Peptide
NAD+ (nicotinamide adenine dinucleotide)
Outcome
DecreaseS. pneumoniae isolation versus two-plate combination
Study
Haemophilus influenzae; Streptococcus pneumoniaen = 1,724
Sputum culture on NAD-supplemented blood agar
Comparator
Plain blood agar plus chocolated blood agar
Outcome group
Infection and antimicrobial activity · Infection
Measurements
Isolation rate
4.18%
NAD-supplemented blood agar
Isolation rate
4.68%
two-plate combination
Source sentences
Summary
The two-plate combination performed significantly better for both organisms; isolation rates for H. influenzae were increased from 8.16% on SBA to 11.07% on BA plus CBA and for S. pneumoniae from 4.18% to 4.68%.
2 context sentences(design, population, supporting numbers)
Summary · context
In the present study, 1724 sputum samples were examined in four laboratories.
Summary · context
The isolation rates of H. influenzae and S. pneumoniae on NAD-supplemented blood agar (SBA) were compared with those on a two-plate combination of plain blood (BA) and chocolated blood agar (CBA).
In this study, NAD+ (nicotinamide adenine dinucleotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking NAD+ (nicotinamide adenine dinucleotide).
Report a problemFND-1059116602
03LabNot a test of NAD+ (nicotinamide adenine dinucleotide) aloneIn vitro (cells)Activity measurement
In sputum cultures on NAD-supplemented blood agar, H. influenzae isolation increased after 48 h compared with 24 h incubation.
In plain wordsIn lab tests, NAD-supplemented blood agar detected more H. influenzae after 48 h than after 24 h.
Peptide
NAD+ (nicotinamide adenine dinucleotide)
Outcome
IncreaseH. influenzae isolation after longer incubation
Study
Haemophilus influenzae; Streptococcus pneumoniaen = 1,724
Sputum culture on NAD-supplemented blood agar
Comparator
24 h incubation
Outcome group
Infection and antimicrobial activity · Infection
Measurements
Isolation rate increase
0.57%
NAD-supplemented blood agar after 48 h
Source sentences
Summary
With the two-plate combination, isolation rates for H. influenzae and S. pneumoniae were increased by 0.98% and 0.16%, respectively, and for SBA by 0.57% and 0.32% after 48 h.
3 context sentences(design, population, supporting numbers)
Summary · context
Isolation rates were also compared after incubation for 24 and 48 h.
Summary · context
In the present study, 1724 sputum samples were examined in four laboratories.
Summary · context
The isolation rates of H. influenzae and S. pneumoniae on NAD-supplemented blood agar (SBA) were compared with those on a two-plate combination of plain blood (BA) and chocolated blood agar (CBA).
In this study, NAD+ (nicotinamide adenine dinucleotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking NAD+ (nicotinamide adenine dinucleotide).
Report a problemFND-1059116603
04LabNot a test of NAD+ (nicotinamide adenine dinucleotide) aloneIn vitro (cells)Activity measurement
In sputum cultures on NAD-supplemented blood agar, S. pneumoniae isolation increased after 48 h compared with 24 h incubation.
In plain wordsIn lab tests, NAD-supplemented blood agar detected more S. pneumoniae after 48 h than after 24 h.
Peptide
NAD+ (nicotinamide adenine dinucleotide)
Outcome
IncreaseS. pneumoniae isolation after longer incubation
Study
Haemophilus influenzae; Streptococcus pneumoniaen = 1,724
Sputum culture on NAD-supplemented blood agar
Comparator
24 h incubation
Outcome group
Infection and antimicrobial activity · Infection
Measurements
Isolation rate increase
0.32%
NAD-supplemented blood agar after 48 h
Source sentences
Summary
With the two-plate combination, isolation rates for H. influenzae and S. pneumoniae were increased by 0.98% and 0.16%, respectively, and for SBA by 0.57% and 0.32% after 48 h.
3 context sentences(design, population, supporting numbers)
Summary · context
Isolation rates were also compared after incubation for 24 and 48 h.
Summary · context
In the present study, 1724 sputum samples were examined in four laboratories.
Summary · context
The isolation rates of H. influenzae and S. pneumoniae on NAD-supplemented blood agar (SBA) were compared with those on a two-plate combination of plain blood (BA) and chocolated blood agar (CBA).
In this study, NAD+ (nicotinamide adenine dinucleotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking NAD+ (nicotinamide adenine dinucleotide).
Report a problemFND-1059116604

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Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
14 Oct 2026, 05:02 UTC
Study results updated
Version 1
14 Oct 2026, 01:46 UTC
First added · source update from Europe PMC

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