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Study · Measured the body's own peptide · 1999

2 results

Polyamine depletion alters the relationship of F-actin, G-actin, and thymosin beta4 in migrating IEC-6 cells.

McCormack SA, Ray RM, Blanner PM, Johnson LR

The American journal of physiology1 Feb 1999Cited by 33 (Europe PMC)

PMID9950774 (opens in a new tab)DOI10.1152/ajpcell.1999.276.2.c459 (opens in a new tab)PepBankPUB-09950774
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study2

No result from this study comes from a test of the peptide.

Natural levels measured2

These results measure the body's own peptide. Nobody was given the peptide. They are not evidence about taking it. They are left out of key results, evidence counts and charts.

  1. 01LabEndogenous levelIn vitro (cells)Mechanism

    In migrating IEC-6 cells, polyamine depletion with DFMO increased nuclear staining of the cells' own Tbeta4.

    In plain wordsIn lab cells, a drug that depletes polyamines increased the cells' own thymosin beta4 staining in the nucleus.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    IncreaseNuclear Tbeta4 staining after polyamine depletion
    Study

    n: not reported in source

    migrating IEC-6 cells
    Comparator
    Control cells
    Outcome group
    Receptor activity and mechanism · Gastrointestinal

    Source sentences

    Summary
    DFMO reduced F-actin in the cell interior, increased it in the cell cortex, redistributed G-actin, and increased nuclear staining of Tbeta4.
    1 context sentence
    Summary · context
    We depleted polyamines with alpha-difluoromethylornithine (DFMO) in migrating cells with or without stimulation by epidermal growth factor (EGF) and investigated filamentous (F-) actin, monomeric (G-) actin, and thymosin beta4 (Tbeta4), using immunofluorescent confocal microscopy, DNase assay, and immunoblot analysis.

    This result measures the body's own Thymosin beta-4 (human). Nobody was given the peptide. It is not evidence about taking it.

  2. 02LabEndogenous levelIn vitro (cells)Mechanism

    In migrating IEC-6 cells, DFMO did not affect the amount of the cells' own Tbeta4 mRNA.

    In plain wordsIn lab cells, a polyamine-depleting drug did not change the amount of the cells' own thymosin beta4 messenger RNA.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    No significant changeTbeta4 mRNA amount after polyamine depletion
    Study

    n: not reported in source

    migrating IEC-6 cells
    Comparator
    Control cells
    Outcome group
    Gene expression · Gastrointestinal

    Source sentences

    Summary
    However, DFMO did not affect the amount of Tbeta4 mRNA.
    1 context sentence
    Summary · context
    We depleted polyamines with alpha-difluoromethylornithine (DFMO) in migrating cells with or without stimulation by epidermal growth factor (EGF) and investigated filamentous (F-) actin, monomeric (G-) actin, and thymosin beta4 (Tbeta4), using immunofluorescent confocal microscopy, DNase assay, and immunoblot analysis.

    This result measures the body's own Thymosin beta-4 (human). Nobody was given the peptide. It is not evidence about taking it.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 01:52 UTC

    Study results updated

  2. Version 1

    12 Oct 2026, 18:17 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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