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Study · Not a test of the peptide alone · 1998

4 results

The dipyridyls paraquat and diquat attenuate the interaction of G-actin with thymosin beta4.

Huff T, Cappelletti G, Hannappel E

FEBS letters1 Apr 1998Cited by 3 (Europe PMC)

PMID9563520 (opens in a new tab)DOI10.1016/s0014-5793(98)00295-6 (opens in a new tab)PepBankPUB-09563520
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study4

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone4

In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Thymosin beta-4 (human).

  1. 01LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cell-free)Activity measurement

    In a cell-free assay, 10 mM paraquat increased the apparent dissociation constant of the G-actin-thymosin beta4 complex 10-85-fold within 15 min to 24 h.

    In plain wordsIn lab tests, paraquat weakened the binding between actin and thymosin beta4.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    IncreaseG-actin-thymosin beta4 dissociation constant with 10 mM paraquat
    Study

    n: not reported in source

    G-actin-thymosin beta4 ultrafiltration assay
    Outcome group
    Receptor activity and mechanism · Pharmacology

    Measurements

    Fold increase in apparent Kd
    10 to 85 fold
    10 mM paraquat, 15 min to 24 h

    Source sentences

    Summary
    When exposed to 10 mM paraquat, the apparent dissociation constant increased 10-85-fold within 15 min to 24 h.
    1 context sentence
    Summary · context
    To study the influence of paraquat binding to G-actin on the interaction of G-actin with thymosin beta4 we determined the apparent dissociation constant of the G-actin-thymosin beta4 complex in the absence or presence of paraquat using an ultrafiltration assay.

    In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).

  2. 02LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cell-free)Activity measurement

    In a cell-free assay, after 24 h incubation 100 microM paraquat increased the dissociation constant of the G-actin-thymosin beta4 complex from 0.66 microM to 0.82 microM.

    In plain wordsIn lab tests, even a low paraquat level weakened the binding between actin and thymosin beta4.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    IncreaseG-actin-thymosin beta4 dissociation constant with 100 microM paraquat
    Study

    n: not reported in source

    G-actin-thymosin beta4 ultrafiltration assay
    Outcome group
    Receptor activity and mechanism · Pharmacology

    Measurements

    Kd without paraquat
    0.66 µM
    24 h
    Kd with paraquat
    0.82 µM
    100 microM paraquat, 24 h

    Source sentences

    Summary
    After incubation for 24 h even a paraquat concentration as low as 100 microM increased the dissociation constant of the G-actin-thymosin beta4 complex from 0.66 microM to 0.82 microM (P < 0.05).
    1 context sentence
    Summary · context
    To study the influence of paraquat binding to G-actin on the interaction of G-actin with thymosin beta4 we determined the apparent dissociation constant of the G-actin-thymosin beta4 complex in the absence or presence of paraquat using an ultrafiltration assay.

    In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).

  3. 03LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cell-free)Activity measurement

    In a cell-free assay, diquat similarly weakened the interaction of G-actin and beta-thymosins.

    In plain wordsIn lab tests, diquat also weakened the binding between actin and beta-thymosins.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    IncreaseG-actin-thymosin beta4 interaction with diquat
    Study

    n: not reported in source

    G-actin-thymosin beta4 ultrafiltration assay
    Outcome group
    Receptor activity and mechanism · Pharmacology

    Source sentences

    Summary
    Diquat (1,1'-ethylene-2,2'-dipyridylium dibromide) similarly weakens the interaction of G-actin and beta-thymosins.
    1 context sentence
    Summary · context
    To study the influence of paraquat binding to G-actin on the interaction of G-actin with thymosin beta4 we determined the apparent dissociation constant of the G-actin-thymosin beta4 complex in the absence or presence of paraquat using an ultrafiltration assay.

    In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).

  4. 04LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cell-free)Mechanism

    In a cell-free assay, no oxidation of the methionine residue or other modification of thymosin beta4 was detected in any experiment.

    In plain wordsIn lab tests, thymosin beta4 showed no chemical change after paraquat or diquat exposure.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    No significant changeModification of thymosin beta4 by paraquat
    Study

    n: not reported in source

    G-actin-thymosin beta4 ultrafiltration assay
    Outcome group
    Receptor activity and mechanism · Pharmacology

    Source sentences

    Summary
    In none of the experiments was oxidation of the methionine residue or any other modification of thymosin beta4 detected.
    1 context sentence
    Summary · context
    To study the influence of paraquat binding to G-actin on the interaction of G-actin with thymosin beta4 we determined the apparent dissociation constant of the G-actin-thymosin beta4 complex in the absence or presence of paraquat using an ultrafiltration assay.

    In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 01:52 UTC

    Study results updated

  2. Version 1

    12 Oct 2026, 18:17 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

Publication types

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