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Study · Not a test of the peptide alone · 1997

10 results

Co-ordinate regulation of the cytoskeleton in 3T3 cells overexpressing thymosin-beta4.

Golla R, Philp N, Safer D, Chintapalli J, Hoffman R, Collins L, Nachmias VT

Cell motility and the cytoskeleton1 Jan 1997Cited by 39 (Europe PMC)

PMID9331222 (opens in a new tab)DOI10.1002/(sici)1097-0169(1997)38:2<187::aid-cm7>3.0.co;2-4 (opens in a new tab)PepBankPUB-09331222
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study10

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone10

In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Thymosin beta-4 (human).

  1. 01LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cells)Mechanism

    In NIH 3T3 cells with twice the control level of Tbeta4, G-actin increased approximately twofold versus vector controls.

    In plain wordsIn mouse 3T3 cells with twice the normal Tβ4 level, free actin (G-actin) rose about twofold versus control cells.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    IncreaseG-actin level
    Study

    mousen: not reported in source

    NIH 3T3 cells overexpressing Tbeta4
    Comparator
    Vector controls
    Outcome group
    Cytoskeleton and cell structure · Whole body

    Source sentences

    Summary
    In cell lines with Tbeta4 levels twice those of vector controls, G-actin increased approximately twofold as expected.
    1 context sentence
    Summary · context
    To determine the extent of cell adaptability to these reductions in F-actin, we overexpressed Tbeta4 in NIH 3T3 cells.

    In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).

  2. 02LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cells)Mechanism

    In NIH 3T3 cells overexpressing Tbeta4, F-actin also increased approximately twofold, rather than decreasing as in short-term experiments.

    In plain wordsIn mouse 3T3 cells with raised Tβ4 gene activity, filamentous actin (F-actin) also rose about twofold.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    IncreaseF-actin level
    Study

    mousen: not reported in source

    NIH 3T3 cells overexpressing Tbeta4
    Comparator
    Vector controls
    Outcome group
    Cytoskeleton and cell structure · Whole body

    Source sentences

    Summary
    However, F-actin did not decrease as in short-term experiments but rather also increased approximately twofold so that the G-F ratio remained constant.
    1 context sentence
    Summary · context
    To determine the extent of cell adaptability to these reductions in F-actin, we overexpressed Tbeta4 in NIH 3T3 cells.

    In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).

  3. 03LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cells)Mechanism

    In NIH 3T3 cells overexpressing Tbeta4, the G-F actin ratio stayed constant because G-actin and F-actin rose together.

    In plain wordsIn mouse 3T3 cells with raised Tβ4 gene activity, the ratio of free to filamentous actin stayed the same because both rose together.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    No significant changeG-F actin ratio
    Study

    mousen: not reported in source

    NIH 3T3 cells overexpressing Tbeta4
    Comparator
    Vector controls
    Outcome group
    Cytoskeleton and cell structure · Whole body

    Source sentences

    Summary
    However, F-actin did not decrease as in short-term experiments but rather also increased approximately twofold so that the G-F ratio remained constant.
    1 context sentence
    Summary · context
    To determine the extent of cell adaptability to these reductions in F-actin, we overexpressed Tbeta4 in NIH 3T3 cells.

    In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).

  4. 04LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cells)Mechanism

    In NIH 3T3 cells overexpressing Tbeta4, myosin IIA, alpha-actinin and tropomyosin each increased nearly twofold.

    In plain wordsIn mouse 3T3 cells with raised Tβ4 gene activity, myosin IIA, alpha-actinin and tropomyosin each rose nearly twofold.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    IncreaseMyosin IIA, alpha-actinin and tropomyosin levels
    Study

    mousen: not reported in source

    NIH 3T3 cells overexpressing Tbeta4
    Comparator
    Vector controls
    Outcome group
    Cytoskeleton and cell structure · Whole body

    Source sentences

    Summary
    Surprisingly, the cytoskeletal proteins myosin IIA, alpha-actinin, and tropomyosin also increased nearly twofold.
    1 context sentence
    Summary · context
    To determine the extent of cell adaptability to these reductions in F-actin, we overexpressed Tbeta4 in NIH 3T3 cells.

    In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).

  5. 05LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cells)Mechanism

    In NIH 3T3 cells overexpressing Tbeta4, DNA, total protein, lactic dehydrogenase, profilin and actin depolymerizing factor levels were unchanged.

    In plain wordsIn mouse 3T3 cells with raised Tβ4 gene activity, DNA, total protein, lactic dehydrogenase, profilin and actin depolymerizing factor did not change.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    No significant changeDNA, total protein, lactic dehydrogenase, profilin and actin depolymerizing factor levels
    Study

    mousen: not reported in source

    NIH 3T3 cells overexpressing Tbeta4
    Comparator
    Vector controls
    Outcome group
    Cytoskeleton and cell structure · Whole body

    Source sentences

    Summary
    These increases were specific; DNA, total protein, lactic dehydrogenase, profilin, and actin depolymerizing factor levels were unchanged in the overexpressing cells.
    1 context sentence
    Summary · context
    To determine the extent of cell adaptability to these reductions in F-actin, we overexpressed Tbeta4 in NIH 3T3 cells.

    In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).

  6. 06LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cells)Efficacy outcome

    NIH 3T3 cells overexpressing Tbeta4 spread more fully and adhered to the dish more strongly than vector controls.

    In plain wordsMouse 3T3 cells with raised Tβ4 gene activity spread out more and stuck to the dish more strongly than control cells.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    IncreaseCell spreading and adhesion
    Study

    mousen: not reported in source

    NIH 3T3 cells overexpressing Tbeta4
    Comparator
    Vector controls
    Outcome group
    Cytoskeleton and cell structure · Whole body

    Source sentences

    Summary
    The Tbeta4 lines spread more fully and adhered to the dish more strongly than vector controls
    1 context sentence
    Summary · context
    To determine the extent of cell adaptability to these reductions in F-actin, we overexpressed Tbeta4 in NIH 3T3 cells.

    In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).

  7. 07LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cells)Mechanism

    In NIH 3T3 cells overexpressing Tbeta4, talin and alpha5-integrin increased twofold.

    In plain wordsIn mouse 3T3 cells with raised Tβ4 gene activity, talin and alpha5-integrin rose twofold.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    IncreaseTalin and alpha5-integrin levels
    Study

    mousen: not reported in source

    NIH 3T3 cells overexpressing Tbeta4
    Comparator
    Vector controls
    Outcome group
    Cytoskeleton and cell structure · Whole body

    Source sentences

    Summary
    this altered phenotype correlated with a twofold increase in talin and alpha5-integrin
    1 context sentence
    Summary · context
    To determine the extent of cell adaptability to these reductions in F-actin, we overexpressed Tbeta4 in NIH 3T3 cells.

    In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).

  8. 08LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cells)Mechanism

    In NIH 3T3 cells overexpressing Tbeta4, vinculin increased nearly threefold.

    In plain wordsIn mouse 3T3 cells with raised Tβ4 gene activity, vinculin rose nearly threefold.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    IncreaseVinculin level
    Study

    mousen: not reported in source

    NIH 3T3 cells overexpressing Tbeta4
    Comparator
    Vector controls
    Outcome group
    Cytoskeleton and cell structure · Whole body

    Source sentences

    Summary
    a nearly threefold increase in vinculin.
    1 context sentence
    Summary · context
    To determine the extent of cell adaptability to these reductions in F-actin, we overexpressed Tbeta4 in NIH 3T3 cells.

    In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).

  9. 09LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cells)Mechanism

    In NIH 3T3 cells overexpressing Tbeta4, focal adhesions detected with antivinculin were larger than in controls.

    In plain wordsIn mouse 3T3 cells with raised Tβ4 gene activity, focal adhesions (cell anchor points) were larger than in control cells.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    IncreaseFocal adhesion size
    Study

    mousen: not reported in source

    NIH 3T3 cells overexpressing Tbeta4
    Comparator
    Vector controls
    Outcome group
    Cytoskeleton and cell structure · Whole body

    Source sentences

    Summary
    Focal adhesions, detected by indirect immunofluorescence with antivinculin, were increased in size over the controls.
    1 context sentence
    Summary · context
    To determine the extent of cell adaptability to these reductions in F-actin, we overexpressed Tbeta4 in NIH 3T3 cells.

    In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).

  10. 10LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cells)Mechanism

    In NIH 3T3 cells overexpressing Tbeta4, mRNAs for beta-actin and vinculin increased twofold.

    In plain wordsIn mouse 3T3 cells with raised Tβ4 gene activity, messenger RNA for beta-actin and vinculin rose twofold.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    IncreaseBeta-actin and vinculin mRNA
    Study

    mousen: not reported in source

    NIH 3T3 cells overexpressing Tbeta4
    Comparator
    Vector controls
    Outcome group
    Cytoskeleton and cell structure · Whole body

    Source sentences

    Summary
    Northern blotting showed that mRNAs for both beta-actin and vinculin were increased twofold in the overexpressing lines.
    1 context sentence
    Summary · context
    To determine the extent of cell adaptability to these reductions in F-actin, we overexpressed Tbeta4 in NIH 3T3 cells.

    In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 01:52 UTC

    Study results updated

  2. Version 1

    12 Oct 2026, 18:17 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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