Determination of a novel growth hormone secretagogue (MK-677) in human plasma at picogram levels by liquid chromatography with atmospheric pressure chemical ionization tandem mass spectrometry.

Constanzer ML, Chavez-Eng CM, Matuszewski BK

Journal of chromatography. B, Biomedical sciences and applications1 May 1997Cited by 11 (Europe PMC)

PMID9200526DOI10.1016/s0378-4347(97)00035-2PepBankPUB-09200526

Publisher page PubMedAsk about this study

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study6

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone6

In this study, MK-677 (ibutamoren) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking MK-677 (ibutamoren).

01LabNot a test of MK-677 (ibutamoren) aloneIn vitro (cell-free)Other
In laboratory tests, the MK‑677 plasma assay was validated over 0.1 to 100 ng/ml.
In plain wordsIn lab tests, the MK‑677 plasma assay was validated over 0.1 to 100 ng/ml.
Peptide
MK-677 (ibutamoren)
Outcome
Not applicableValidated concentration range
Study
n: not reported in source
Human plasma assay
Outcome group
Analytical methods and detection · Pharmacology
Measurements
Validated concentration range
0.1 to 100 ng/ml
Human plasma
Source sentences
Summary
The assay was validated in human plasma in the concentration range of 0.1 to 100 ng/ml, and the limit of quantification (LOQ) was 0.1 ng/ml.
2 context sentences(design, population, supporting numbers)
Summary · context
The method is based on high-performance liquid chromatography (HPLC) with tandem mass spectrometric (MS-MS) detection.
Summary · context
The analyte and internal standard (II) were isolated from the basified plasma using a liquid-liquid extraction with methyl-tert.-butyl ether (MTBE).
In this study, MK-677 (ibutamoren) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking MK-677 (ibutamoren).
Report a problemFND-0920052601
02LabNot a test of MK-677 (ibutamoren) aloneIn vitro (cell-free)Other
In laboratory tests, the MK‑677 plasma assay had a limit of quantification of 0.1 ng/ml.
In plain wordsIn lab tests, the MK‑677 plasma assay measured concentrations as low as 0.1 ng/ml.
Peptide
MK-677 (ibutamoren)
Outcome
Not applicableLimit of quantification
Study
n: not reported in source
Human plasma assay
Outcome group
Analytical methods and detection · Pharmacology
Measurements
Limit of quantification
0.1 ng/ml
Human plasma
Source sentences
Summary
The assay was validated in human plasma in the concentration range of 0.1 to 100 ng/ml, and the limit of quantification (LOQ) was 0.1 ng/ml.
2 context sentences(design, population, supporting numbers)
Summary · context
The method is based on high-performance liquid chromatography (HPLC) with tandem mass spectrometric (MS-MS) detection.
Summary · context
The analyte and internal standard (II) were isolated from the basified plasma using a liquid-liquid extraction with methyl-tert.-butyl ether (MTBE).
In this study, MK-677 (ibutamoren) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking MK-677 (ibutamoren).
Report a problemFND-0920052602
03LabNot a test of MK-677 (ibutamoren) aloneIn vitro (cell-free)Other
In laboratory tests, MK‑677 assay coefficients of variation were less than 7% across the standard curve.
In plain wordsIn lab tests, MK‑677 assay coefficients of variation were less than 7% across the standard curve.
Peptide
MK-677 (ibutamoren)
Outcome
Not applicableAssay precision
Study
n: not reported in source
Human plasma assay
Outcome group
Analytical methods and detection · Pharmacology
Measurements
Coefficient of variation
< 7%
All standard curve concentrations
Source sentences
Summary
The precision of the assay, as expressed as coefficients of variation (C.V.,%) was less than 7% at all concentrations within the standard curve range, with adequate assay specificity and accuracy.
2 context sentences(design, population, supporting numbers)
Summary · context
The method is based on high-performance liquid chromatography (HPLC) with tandem mass spectrometric (MS-MS) detection.
Summary · context
The analyte and internal standard (II) were isolated from the basified plasma using a liquid-liquid extraction with methyl-tert.-butyl ether (MTBE).
In this study, MK-677 (ibutamoren) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking MK-677 (ibutamoren).
Report a problemFND-0920052603
04LabNot a test of MK-677 (ibutamoren) aloneIn vitro (cell-free)Other
In laboratory tests, the MK‑677 plasma assay had adequate specificity.
In plain wordsIn lab tests, the MK‑677 plasma assay had adequate specificity.
Peptide
MK-677 (ibutamoren)
Outcome
Not applicableAssay specificity
Study
n: not reported in source
Human plasma assay
Outcome group
Analytical methods and detection · Pharmacology
Source sentences
Summary
The precision of the assay, as expressed as coefficients of variation (C.V.,%) was less than 7% at all concentrations within the standard curve range, with adequate assay specificity and accuracy.
2 context sentences(design, population, supporting numbers)
Summary · context
The method is based on high-performance liquid chromatography (HPLC) with tandem mass spectrometric (MS-MS) detection.
Summary · context
The analyte and internal standard (II) were isolated from the basified plasma using a liquid-liquid extraction with methyl-tert.-butyl ether (MTBE).
In this study, MK-677 (ibutamoren) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking MK-677 (ibutamoren).
Report a problemFND-0920052604
05LabNot a test of MK-677 (ibutamoren) aloneIn vitro (cell-free)Other
In laboratory tests, the MK‑677 plasma assay had adequate accuracy.
In plain wordsIn lab tests, the MK‑677 plasma assay had adequate accuracy.
Peptide
MK-677 (ibutamoren)
Outcome
Not applicableAssay accuracy
Study
n: not reported in source
Human plasma assay
Outcome group
Analytical methods and detection · Pharmacology
Source sentences
Summary
The precision of the assay, as expressed as coefficients of variation (C.V.,%) was less than 7% at all concentrations within the standard curve range, with adequate assay specificity and accuracy.
2 context sentences(design, population, supporting numbers)
Summary · context
The method is based on high-performance liquid chromatography (HPLC) with tandem mass spectrometric (MS-MS) detection.
Summary · context
The analyte and internal standard (II) were isolated from the basified plasma using a liquid-liquid extraction with methyl-tert.-butyl ether (MTBE).
In this study, MK-677 (ibutamoren) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking MK-677 (ibutamoren).
Report a problemFND-0920052605
06LabNot a test of MK-677 (ibutamoren) aloneIn vitro (cell-free)Other
The MK‑677 assay had sufficient sensitivity to map the pharmacokinetic time-course after a single oral dose.
In plain wordsIn lab tests, the MK‑677 assay had sufficient sensitivity to map its pharmacokinetic time-course after a single oral dose.
Peptide
MK-677 (ibutamoren)
Outcome
Not applicablePharmacokinetic mapping sensitivity
Study
n: not reported in source
Human plasma assay
Outcome group
Analytical methods and detection · Pharmacology
Source sentences
Summary
The HPLC-MS-MS method provided sufficient sensitivity to completely map the pharmacokinetic time-course following a single 5-mg oral dose of I.
2 context sentences(design, population, supporting numbers)
Summary · context
The method is based on high-performance liquid chromatography (HPLC) with tandem mass spectrometric (MS-MS) detection.
Summary · context
The analyte and internal standard (II) were isolated from the basified plasma using a liquid-liquid extraction with methyl-tert.-butyl ether (MTBE).
In this study, MK-677 (ibutamoren) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking MK-677 (ibutamoren).
Report a problemFND-0920052606

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
14 Oct 2026, 05:02 UTC
Study results updated
Version 1
14 Oct 2026, 01:46 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

Publication types

Journal Article

MeSH terms

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