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Study · In vitro (cells) · mouse · 1996

9 results

Binding and internalization of the melanocyte stimulating hormone receptor ligand [Nle4, D-Phe7] alpha-MSH in B16 melanoma cells.

Wong W, Minchin RF

The international journal of biochemistry & cell biology1 Nov 1996Cited by 23 (Europe PMC)

PMID9022281 (opens in a new tab)DOI10.1016/s1357-2725(96)00074-x (opens in a new tab)PepBankPUB-09022281
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study9

9 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Mechanism

    In B16 melanoma cells, NDP-MSH accumulation increased with time in a temperature-dependent manner and excess peptide inhibited it.

    In plain wordsIn mouse melanoma cells, NDP-MSH built up over time, depending on temperature, and extra peptide blocked this.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    IncreaseNDP-MSH cellular accumulation
    Study

    mousen: not reported in source

    B16 melanoma cells
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    NDP-MSH accumulation increased with time in a temperature-dependent manner and was inhibited by excess peptide.
    1 context sentence
    Summary · context
    Cells were incubated with [125I]-NDP-MSH for up to 180 min and binding, internalization and degradation determined.
  2. 02LabIn vitro (cells)Mechanism

    In B16 melanoma cells, NDP-MSH was rapidly internalized and moved to the lysosomal compartment where it was degraded.

    In plain wordsIn mouse melanoma cells, NDP-MSH was quickly taken up and broken down inside the cells.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    Not applicableInternalization and lysosomal degradation
    Study

    mousen: not reported in source

    B16 melanoma cells
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    The ligand was rapidly internalized and translocated to the lysosomal compartment where it was degraded.
    1 context sentence
    Summary · context
    Cells were incubated with [125I]-NDP-MSH for up to 180 min and binding, internalization and degradation determined.
  3. 03LabIn vitro (cells)Mechanism

    In B16 melanoma cells, NDP-MSH internalization came with a loss of cell surface MSH receptors, which suggests internalization of the ligand-receptor complex.

    In plain wordsIn mouse melanoma cells, NDP-MSH lowered the number of MSH receptors on the cell surface.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    DecreaseCell surface receptor level
    Study

    mousen: not reported in source

    B16 melanoma cells
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Internalization was accompanied by a loss or down-regulation of cell surface receptors, suggesting internalization of the NDP-MSH-receptor complex.
    1 context sentence
    Summary · context
    Cells were incubated with [125I]-NDP-MSH for up to 180 min and binding, internalization and degradation determined.
  4. 04LabIn vitro (cells)Mechanism

    In B16 melanoma cells, no recycling of MSH receptors between the plasma membrane and intracellular compartments was detected after NDP-MSH.

    In plain wordsIn mouse melanoma cells, no recycling of the MSH receptor back to the surface was detected.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    No significant changeReceptor recycling
    Study

    mousen: not reported in source

    B16 melanoma cells
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    No recycling of the receptors between the plasma membrane and intracellular compartments could be detected in this cell-line.
    1 context sentence
    Summary · context
    Cells were incubated with [125I]-NDP-MSH for up to 180 min and binding, internalization and degradation determined.
  5. 05LabIn vitro (cells)Mechanism

    In B16 melanoma cells, approximately 15% of surface receptors were resistant to NDP-MSH-induced down-regulation.

    In plain wordsIn mouse melanoma cells, about 15% of the surface receptors did not go down after NDP-MSH.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    Not applicableProportion of receptors resistant to down-regulation
    Study

    mousen: not reported in source

    B16 melanoma cells
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Measurements

    Receptors resistant to down-regulation
    ≈ 15%
    B16 cells

    Source sentences

    Summary
    Approximately 15% of the surface receptors were resistant to down-regulation, possibly indicating receptor heterogeneity.
    1 context sentence
    Summary · context
    Cells were incubated with [125I]-NDP-MSH for up to 180 min and binding, internalization and degradation determined.
  6. 06LabIn vitro (cells)Mechanism

    In B16 melanoma cells, MSH receptor down-regulation persisted for up to 96 hr after exposure to 1 microM NDP-MSH.

    In plain wordsIn mouse melanoma cells, the drop in MSH receptors after NDP-MSH lasted up to 96 hours.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    DecreaseDuration of receptor down-regulation
    Study

    mousen: not reported in source

    B16 melanoma cells
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    1 microM · up to 96 hrAs given in this study (mouse).

    Measurements

    Down-regulation duration
    ≤ 96 hr
    B16 cells

    Source sentences

    Summary
    Down-regulation persisted for up to 96 hr
    2 context sentences
    Summary · context
    Receptor down-regulation and receptor mRNA levels were also measured over 96 hr after exposure to 1 microM ligand.
    Summary · context
    Cells were incubated with [125I]-NDP-MSH for up to 180 min and binding, internalization and degradation determined.
  7. 07LabIn vitro (cells)Mechanism

    In B16 melanoma cells, MSH receptor mRNA levels were lower 48 hr after NDP-MSH treatment.

    In plain wordsIn mouse melanoma cells, MSH receptor mRNA was lower 48 hours after NDP-MSH treatment.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    DecreaseMSH receptor mRNA level at 48 hr
    Study

    mousen: not reported in source

    B16 melanoma cells
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    1 microM · 48 hrAs given in this study (mouse).

    Measurements

    Time of mRNA decrease
    48 hr
    B16 cells

    Source sentences

    Summary
    Down-regulation persisted for up to 96 hr and was accompanied by a decrease in MSH receptor mRNA levels 48 hr after treatment.
    1 context sentence
    Summary · context
    Cells were incubated with [125I]-NDP-MSH for up to 180 min and binding, internalization and degradation determined.
  8. 08LabIn vitro (cells)Mechanism

    In B16 melanoma cells, MSH receptor transcript levels did not differ between treated and control cells before 48 hr.

    In plain wordsIn mouse melanoma cells, receptor mRNA was the same in treated and untreated cells before 48 hours.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    No significant changeMSH receptor mRNA level before 48 hr
    Study

    mousen: not reported in source

    B16 melanoma cells
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    1 microMAs given in this study (mouse).

    Source sentences

    Summary
    However, before this time, transcript levels were the same in treated and control cells.
    1 context sentence
    Summary · context
    Cells were incubated with [125I]-NDP-MSH for up to 180 min and binding, internalization and degradation determined.
  9. 09LabIn vitro (cells)Mechanism

    In B16 melanoma cells, NDP-MSH inhibited MSH receptor turnover and gave prolonged down-regulation, whereas trypsin-removed cell surface receptors were rapidly replaced.

    In plain wordsIn mouse melanoma cells, NDP-MSH slowed the replacement of MSH receptors, so the drop lasted longer.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    DecreaseReceptor turnover
    Study

    mousen: not reported in source

    B16 melanoma cells
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    In contrast to what was seen with NDP-MSH, cell surface receptors removed with trypsin were rapidly replaced. These results show that NDP-MSH not only induced MSH receptor internalization but also inhibited receptor turnover, resulting in a prolonged down-regulation.
    1 context sentence
    Summary · context
    Cells were incubated with [125I]-NDP-MSH for up to 180 min and binding, internalization and degradation determined.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 21:44 UTC

    Study results updated

  2. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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