Matrigel induces thymosin beta 4 gene in differentiating endothelial cells.

Grant DS, Kinsella JL, Kibbey MC, LaFlamme S, Burbelo PD, Goldstein AL, Kleinman HK

Journal of cell science1 Dec 1995Cited by 117 (Europe PMC)

PMID8719875DOI10.1242/jcs.108.12.3685PepBankPUB-08719875

Publisher page PubMedAsk about this study

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study5

No result from this study comes from a test of the peptide.

Natural levels measured2

These results measure the body's own peptide. Nobody was given the peptide. They are not evidence about taking it. They are left out of key results, evidence counts and charts.

01LabEndogenous levelEx vivo tissueMechanism
Immunostaining localized thymosin beta‑4 in vivo in both growing and mature vessels as well as in other tissues.
In plain wordsStaining found the body's own thymosin beta‑4 in growing and mature blood vessels and in other tissues.
Peptide
Thymosin beta-4 (human)
Outcome
Not applicableThymosin beta 4 immunostaining in vessels and tissues
Study
n: not reported in source
Tissue immunostaining
Outcome group
Angiogenesis · Cardiovascular
Source sentences
Summary
Immunostaining localized thymosin beta 4 in vivo in both growing and mature vessels as well as in other tissues.
This result measures the body's own Thymosin beta-4 (human). Nobody was given the peptide. It is not evidence about taking it.
Report a problemFND-0871987502
02LabEndogenous levelIn vitro (cells)Mechanism
In endothelial cells cultured on Matrigel, thymosin beta‑4 mRNA increased 5-fold.
In plain wordsIn endothelial cells grown on Matrigel, the cells' own thymosin beta‑4 mRNA rose 5-fold compared with plastic.
Peptide
Thymosin beta-4 (human)
Outcome
IncreaseThymosin beta 4 mRNA on Matrigel
Study
n: not reported in source
Endothelial cells on plastic or Matrigel
Comparator
Plastic
Outcome group
Angiogenesis · Cardiovascular
Measurements
mRNA fold increase
5 fold
endothelial cells on Matrigel
Source sentences
Summary
The mRNA for one clone, thymosin beta 4, was increased 5-fold.
1 context sentence(design, population, supporting numbers)
Summary · context
We performed differential cDNA hybridization using RNA from endothelial cells cultured for 4 hours on either plastic or basement membrane matrix (Matrigel), and identified early genes induced during the morphological differentiation into capillary-like tubes.
This result measures the body's own Thymosin beta-4 (human). Nobody was given the peptide. It is not evidence about taking it.
Report a problemFND-0871987501

Studies that did not test the peptide alone3

In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Thymosin beta-4 (human).

03LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cells)Mechanism
Endothelial cells transfected with thymosin beta‑4 showed an increased rate of attachment and spreading on matrix components.
In plain wordsIn endothelial cells, adding extra thymosin beta‑4 by gene transfer made the cells attach and spread faster on matrix components.
Peptide
Thymosin beta-4 (human)
Outcome
IncreaseAttachment and spreading of thymosin beta 4-transfected cells
Study
n: not reported in source
Endothelial cells on plastic or Matrigel
Outcome group
Angiogenesis · Cardiovascular
Source sentences
Summary
Endothelial cells transfected with thymosin beta 4 showed an increased rate of attachment and spreading on matrix components
1 context sentence(design, population, supporting numbers)
Summary · context
We performed differential cDNA hybridization using RNA from endothelial cells cultured for 4 hours on either plastic or basement membrane matrix (Matrigel), and identified early genes induced during the morphological differentiation into capillary-like tubes.
In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).
Report a problemFND-0871987503
04LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cells)Mechanism
Endothelial cells transfected with thymosin beta‑4 showed an accelerated rate of tube formation on Matrigel.
In plain wordsIn endothelial cells, extra thymosin beta‑4 from gene transfer made tube formation on Matrigel faster.
Peptide
Thymosin beta-4 (human)
Outcome
IncreaseTube formation of thymosin beta 4-transfected cells
Study
n: not reported in source
Endothelial cells on plastic or Matrigel
Outcome group
Angiogenesis · Cardiovascular
Source sentences
Summary
Endothelial cells transfected with thymosin beta 4 showed an increased rate of attachment and spreading on matrix components, and an accelerated rate of tube formation on Matrigel.
1 context sentence(design, population, supporting numbers)
Summary · context
We performed differential cDNA hybridization using RNA from endothelial cells cultured for 4 hours on either plastic or basement membrane matrix (Matrigel), and identified early genes induced during the morphological differentiation into capillary-like tubes.
In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).
Report a problemFND-0871987504
05LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cells)Mechanism
An antisense oligo to thymosin beta‑4 inhibited tube formation of endothelial cells on Matrigel.
In plain wordsIn endothelial cells, blocking thymosin beta‑4 with an antisense oligo reduced tube formation on Matrigel.
Peptide
Thymosin beta-4 (human)
Outcome
DecreaseTube formation with antisense oligo
Study
n: not reported in source
Endothelial cells on plastic or Matrigel
Outcome group
Angiogenesis · Cardiovascular
Source sentences
Summary
An antisense oligo to thymosin beta 4 inhibited tube formation on Matrigel.
1 context sentence(design, population, supporting numbers)
Summary · context
We performed differential cDNA hybridization using RNA from endothelial cells cultured for 4 hours on either plastic or basement membrane matrix (Matrigel), and identified early genes induced during the morphological differentiation into capillary-like tubes.
In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).
Report a problemFND-0871987505

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
13 Oct 2026, 01:52 UTC
Study results updated
Version 1
12 Oct 2026, 18:17 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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