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Study · In vitro (cells) · 1996

4 results

Desensitization of melanotropin receptors in COS-7 cells.

Polgar WE, Bitler CM, Toll L

Life sciences1 Jan 1996Cited by 4 (Europe PMC)

PMID8614275 (opens in a new tab)DOI10.1016/0024-3205(96)00083-5 (opens in a new tab)PepBankPUB-08614275
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study4

4 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Activity measurement

    In COS-7 cells, NDP-alpha-MSH acted as a partial agonist and stimulated cAMP accumulation to approximately 50% of the level of alpha-MSH, beta-MSH and ACTH.

    In plain wordsIn cells, NDP-alpha-MSH activated cells only about half as much as alpha-MSH, beta-MSH and ACTH.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    Not applicablecAMP accumulation relative to alpha-MSH, beta-MSH and ACTH
    Study

    n: not reported in source

    COS-7 cells
    Comparator
    alpha-MSH, beta-MSH and ACTH
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Measurements

    cAMP accumulation relative to alpha-MSH
    ≈ 50%
    COS-7 cells

    Source sentences

    Summary
    Surprisingly, NDP-alpha-MSH did not act as a full agonist in COS-7 cells. In multiple experiments, it could stimulate cAMP accumulation to approximately 50% of the level of alpha-MSH, beta-MSH and adrenocorticotropic hormone (ACTH).
    1 context sentence
    Summary · context
    In these cells we studied the ultra-long lasting super agonist [Nle(4)-D-Phe(7)]-alpha-MSH (NDP-alpha-MSH), and compared it with the endogenous MSH peptides with respect to potency, maximal activity, duration of action, and rate of desensitization.
  2. 02LabIn vitro (cells)Activity measurement

    In COS-7 cells, NDP-alpha-MSH did not induce prolonged cAMP accumulation.

    In plain wordsIn cells, NDP-alpha-MSH did not keep the cells activated for a long time.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    No significant changeDuration of cAMP accumulation
    Study

    n: not reported in source

    COS-7 cells
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    In contrast to other systems NDP-alpha-MSH did not induce prolonged activity, with respect to cAMP accumulation, in COS-7 cells.
    1 context sentence
    Summary · context
    In these cells we studied the ultra-long lasting super agonist [Nle(4)-D-Phe(7)]-alpha-MSH (NDP-alpha-MSH), and compared it with the endogenous MSH peptides with respect to potency, maximal activity, duration of action, and rate of desensitization.
  3. 03LabIn vitro (cells)Activity measurement

    In COS-7 cells, the activity of NDP-alpha-MSH desensitized more rapidly than that of any of the full agonists.

    In plain wordsIn cells, NDP-alpha-MSH lost its effect faster than the full-strength MSH peptides.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    IncreaseRate of desensitization of NDP-alpha-MSH activity
    Study

    n: not reported in source

    COS-7 cells
    Comparator
    Full agonist MSH peptides
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    As expected for a partial agonist, the activity of NDP-alpha-MSH desensitized more rapidly than any of the full agonists.
    1 context sentence
    Summary · context
    In these cells we studied the ultra-long lasting super agonist [Nle(4)-D-Phe(7)]-alpha-MSH (NDP-alpha-MSH), and compared it with the endogenous MSH peptides with respect to potency, maximal activity, duration of action, and rate of desensitization.
  4. 04LabIn vitro (cells)Activity measurement

    In COS-7 cells, desensitization induced by NDP-alpha-MSH pretreatment occurred more rapidly than desensitization induced by the other MSH analogs.

    In plain wordsIn cells, pretreatment with NDP-alpha-MSH made the cells stop responding faster than pretreatment with other MSH peptides.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    IncreaseRate of desensitization induced by NDP-alpha-MSH pretreatment
    Study

    n: not reported in source

    COS-7 cells
    Comparator
    Other MSH analogs
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Surprisingly, desensitization induced by pretreatment with NDP-alpha-MSH also occurred more rapidly than desensitization induced by the other MSH analogs.
    1 context sentence
    Summary · context
    In these cells we studied the ultra-long lasting super agonist [Nle(4)-D-Phe(7)]-alpha-MSH (NDP-alpha-MSH), and compared it with the endogenous MSH peptides with respect to potency, maximal activity, duration of action, and rate of desensitization.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 21:44 UTC

    Study results updated

  2. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

Subjects

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