Skip to content

Study · Animal study (in vivo) · rat · 1993

5 results

Alpha-melanocyte-stimulating hormone (MSH) and [Nle4,D-Phe7]-alpha-MSH: effects on core temperature in rats.

Raible LH, Knickerbocker D

Pharmacology, biochemistry, and behavior1 Mar 1993Cited by 14 (Europe PMC)

PMID8383852 (opens in a new tab)DOI10.1016/0091-3057(93)90163-n (opens in a new tab)PepBankPUB-08383852
Publisher pagePubMedAsk about this study
Jump to 5 results

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study5

3 results from studies that gave the peptide. No result from this study comes from people.

  1. 01AnimalAnimal study (in vivo)Efficacy outcome

    In rats, 5 pM NDP-MSH produced a significantly higher core temperature than sterile saline.

    In plain wordsIn rats, NDP-MSH injected into a brain area gave a higher core temperature than saline.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    IncreaseCore temperature versus saline (Experiment 2)
    Study

    ratn = 13

    rats with injection into the anterior hypothalamic-preoptic area
    Comparator
    Saline
    Outcome group
    Body temperature · Whole body
    Studied dose
    5 pM in 0.5 microliters · injection into the anterior hypothalamic-preoptic areaAs given in this study (rat).

    Source sentences

    Summary
    Mean temperature of animals receiving NDP-MSH, the 4-10, or the 7-13 fragments, did not differ from each other but were significantly greater than mean temperatures for animals receiving sterile saline or the 1-7 fragment of NDP-MSH (p < 0.05).
    3 context sentences
    Summary · context
    In Experiment 2, subjects received 5 pM NDP-MSH1-7, NDP-MSH4-10, NDP-MSH7-13, NDP-MSH, or the vehicle, 0.5 microliters sterile saline, in a counterbalanced fashion (n = 13).
    Summary · context
    An analysis of the data from Experiment 2 indicated a significant effect of substance, F(4, 48) = 17.31, p < 0.001.
    Summary · context
    The thermoregulatory effects of alpha-melanocyte stimulating hormone (MSH), its potent analog, [Nle4,D-Phe7]-alpha-MSH (NDP-MSH), and the 1-7, 4-10, and 7-13 amino acid fragments of NDP-MSH were examined by administering these substances to the anterior hypothalamic-preoptic area (AHPOA) of rats.
  2. 02AnimalAnimal study (in vivo)Efficacy outcome

    In rats, NDP-MSH injected into the anterior hypothalamic-preoptic area had a significant dose effect on core temperature.

    In plain wordsIn rats, NDP-MSH injected into a brain area changed core body temperature depending on the dose.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    IncreaseCore temperature, dose effect (Experiment 1b)
    Study

    ratn: not reported in source

    rats with injection into the anterior hypothalamic-preoptic area
    Outcome group
    Body temperature · Whole body
    Studied dose
    0, 0.5, 1, 5, 10 or 50 pM in 0.5 microliters · injection into the anterior hypothalamic-preoptic area · 60 min recordingAs given in this study (rat).

    Measurements

    F(5,30)
    4.98
    NDP-MSH dose effect
    p
    0.002
    NDP-MSH dose effect

    Source sentences

    Summary
    Results indicated a significant effect of dose for both MSH, F(5, 30) = 2.81, p = 0.03, and NDP-MSH, F(5, 30) = 4.98, p = 0.002.
    2 context sentences
    Summary · context
    In Experiments 1a (MSH) and 1b (NDP-MSH), animals received 0, 0.5, 1, 5, 10, or 50 pM peptide in 0.5 microliters sterile saline (n = 6/group), with core rectal temperatures being recorded 0, 10, 20, 30, 40, 50, and 60 min after injection.
    Summary · context
    The thermoregulatory effects of alpha-melanocyte stimulating hormone (MSH), its potent analog, [Nle4,D-Phe7]-alpha-MSH (NDP-MSH), and the 1-7, 4-10, and 7-13 amino acid fragments of NDP-MSH were examined by administering these substances to the anterior hypothalamic-preoptic area (AHPOA) of rats.
  3. 03AnimalAnimal study (in vivo)Efficacy outcome

    In rats, core temperatures after every NDP-MSH dose were significantly higher than after saline.

    In plain wordsIn rats, every dose of NDP-MSH injected into a brain area gave a higher core temperature than saline.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    IncreaseCore temperature versus saline (Experiment 1b)
    Study

    ratn: not reported in source

    rats with injection into the anterior hypothalamic-preoptic area
    Comparator
    Saline
    Outcome group
    Body temperature · Whole body
    Studied dose
    0.5 to 50 pM in 0.5 microliters · injection into the anterior hypothalamic-preoptic area · 60 min recordingAs given in this study (rat).

    Source sentences

    Summary
    A Newman-Keul's analysis indicated that mean temperatures for all groups receiving MSH or NDP-MSH were significantly greater than for the group that received saline (p < 0.05).
    1 context sentence
    Summary · context
    The thermoregulatory effects of alpha-melanocyte stimulating hormone (MSH), its potent analog, [Nle4,D-Phe7]-alpha-MSH (NDP-MSH), and the 1-7, 4-10, and 7-13 amino acid fragments of NDP-MSH were examined by administering these substances to the anterior hypothalamic-preoptic area (AHPOA) of rats.

Studies that did not test the peptide alone2

In this study, Melanotan I (afamelanotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Melanotan I (afamelanotide).

  1. 04AnimalNot a test of Melanotan I (afamelanotide) aloneAnimal study (in vivo)Efficacy outcome

    In rats, the 4-10 and 7-13 fragments of NDP-MSH raised core temperature as much as NDP-MSH and more than saline.

    In plain wordsIn rats, two shorter pieces of NDP-MSH raised core temperature as much as the full peptide and more than saline.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    IncreaseCore temperature after NDP-MSH fragments 4-10 and 7-13
    Study

    ratn = 13

    rats with injection into the anterior hypothalamic-preoptic area
    Comparator
    Saline
    Outcome group
    Body temperature · Whole body

    Source sentences

    Summary
    Mean temperature of animals receiving NDP-MSH, the 4-10, or the 7-13 fragments, did not differ from each other but were significantly greater than mean temperatures for animals receiving sterile saline or the 1-7 fragment of NDP-MSH (p < 0.05).
    2 context sentences
    Summary · context
    In Experiment 2, subjects received 5 pM NDP-MSH1-7, NDP-MSH4-10, NDP-MSH7-13, NDP-MSH, or the vehicle, 0.5 microliters sterile saline, in a counterbalanced fashion (n = 13).
    Summary · context
    The thermoregulatory effects of alpha-melanocyte stimulating hormone (MSH), its potent analog, [Nle4,D-Phe7]-alpha-MSH (NDP-MSH), and the 1-7, 4-10, and 7-13 amino acid fragments of NDP-MSH were examined by administering these substances to the anterior hypothalamic-preoptic area (AHPOA) of rats.

    In this study, Melanotan I (afamelanotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Melanotan I (afamelanotide).

  2. 05AnimalNot a test of Melanotan I (afamelanotide) aloneAnimal study (in vivo)Efficacy outcome

    In rats, the 1-7 fragment of NDP-MSH did not raise core temperature above the saline level.

    In plain wordsIn rats, the shortest piece of NDP-MSH (amino acids 1-7) did not raise core temperature compared with saline.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    No significant changeCore temperature after NDP-MSH fragment 1-7
    Study

    ratn = 13

    rats with injection into the anterior hypothalamic-preoptic area
    Comparator
    Saline
    Outcome group
    Body temperature · Whole body

    Source sentences

    Summary
    Mean temperature of animals receiving NDP-MSH, the 4-10, or the 7-13 fragments, did not differ from each other but were significantly greater than mean temperatures for animals receiving sterile saline or the 1-7 fragment of NDP-MSH (p < 0.05).
    2 context sentences
    Summary · context
    In Experiment 2, subjects received 5 pM NDP-MSH1-7, NDP-MSH4-10, NDP-MSH7-13, NDP-MSH, or the vehicle, 0.5 microliters sterile saline, in a counterbalanced fashion (n = 13).
    Summary · context
    The thermoregulatory effects of alpha-melanocyte stimulating hormone (MSH), its potent analog, [Nle4,D-Phe7]-alpha-MSH (NDP-MSH), and the 1-7, 4-10, and 7-13 amino acid fragments of NDP-MSH were examined by administering these substances to the anterior hypothalamic-preoptic area (AHPOA) of rats.

    In this study, Melanotan I (afamelanotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Melanotan I (afamelanotide).

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 21:44 UTC

    Study results updated

  2. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

PepBank is a research reference. Talk to a doctor before you use any peptide.