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Study · In vitro (cell-free) · 1993

2 results

Use of bimanyl actin derivative (TMB-actin) for studying complexation of beta-thymosins. Inhibition of actin polymerization by thymosin beta 9.

Heintz D, Reichert A, Mihelic M, Voelter W, Faulstich H

FEBS letters1 Aug 1993Cited by 42 (Europe PMC)

PMID8354414 (opens in a new tab)DOI10.1016/0014-5793(93)80181-s (opens in a new tab)PepBankPUB-08354414
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study2

2 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cell-free)Activity measurement

    In a cell-free assay with TMB-labelled rabbit muscle actin, thymosin beta‑4 significantly decreased the fluorescence of TMB-actin.

    In plain wordsIn lab tests with purified actin, thymosin beta‑4 lowered the fluorescence signal of labelled actin.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    DecreaseTMB-actin fluorescence after thymosin beta 4
    Study

    n: not reported in source

    Rabbit muscle actin with TMB reporter group
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Fluorescence of TMB-actin decreased significantly on addition of thymosin beta 4 (T beta 4), a peptide of 43 amino acid residues reported to bind to monomeric actin and to prevent filament formation.
  2. 02LabIn vitro (cell-free)Activity measurement

    In a cell-free assay, the KD value of the thymosin beta‑4 complex with actin was 0.8 microM.

    In plain wordsIn lab tests, thymosin beta‑4 bound actin with a KD of 0.8 microM.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    Not applicableKD of thymosin beta 4-actin complex
    Study

    n: not reported in source

    Rabbit muscle actin with TMB reporter group
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Measurements

    KD
    0.8 microM
    thymosin beta 4-actin complex

    Source sentences

    Summary
    Based on this effect, we determined the KD value of the thymosin beta 4 complex as 0.8 microM, a value that is in agreement with previous determinations.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 01:52 UTC

    Study results updated

  2. Version 1

    12 Oct 2026, 18:17 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

Publication types

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