Molecular cloning and characterization of the rat fifth melanocortin receptor.

Griffon N, Mignon V, Facchinetti P, Diaz J, Schwartz JC, Sokoloff P

Biochemical and biophysical research communications1 Apr 1994Cited by 151 (Europe PMC)

PMID8179577DOI10.1006/bbrc.1994.1550PepBankPUB-08179577

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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study2

2 results from studies that gave the peptide. No result from this study comes from people.

01LabIn vitro (cells)Activity measurement
In CHO cells expressing the rat MC5 receptor, NDP-alpha-MSH increased cAMP accumulation.
In plain wordsIn cells carrying the rat MC5 receptor, NDP-alpha-MSH raised cAMP levels.
Peptide
Melanotan I (afamelanotide)
Outcome
IncreasecAMP accumulation at the MC5 receptor
Study
rat receptorn: not reported in source
CHO cells stably expressing the rat MC5 receptor
Outcome group
Receptor activity and mechanism · Pharmacology
Studied dose
Dose not stated
Source sentences
Summary
The MC5, stably expressed in CHO cells, mediates increase in cAMP accumulation with a characteristic pharmacology
1 context sentence(design, population, supporting numbers)
Summary · context
We report here the cloning, pharmacological characterization and expression of the rat fifth melanocortin receptor (MC5), starting from the dopamine D3 receptor sequence to screen a genomic DNA library.
Report a problemFND-0817957701
02LabIn vitro (cells)Activity measurement
At the rat MC5 receptor in CHO cells, alpha-MSH was twice as potent as NDP-alpha-MSH, and ACTH and gamma-MSH were less potent.
In plain wordsIn cells carrying the rat MC5 receptor, NDP-alpha-MSH was about half as potent as alpha-MSH.
Peptide
Melanotan I (afamelanotide)
Outcome
Not applicableRelative potency at the MC5 receptor
Study
rat receptorn: not reported in source
CHO cells stably expressing the rat MC5 receptor
Outcome group
Receptor activity and mechanism · Pharmacology
Studied dose
Dose not stated
Source sentences
Summary
alpha MSH is twice as potent as NDP alpha MSH, 10 times as ACTH and 100 times as gamma MSH
1 context sentence(design, population, supporting numbers)
Summary · context
We report here the cloning, pharmacological characterization and expression of the rat fifth melanocortin receptor (MC5), starting from the dopamine D3 receptor sequence to screen a genomic DNA library.
Report a problemFND-0817957702

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Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
13 Oct 2026, 21:44 UTC
Study results updated
Version 1
13 Oct 2026, 16:31 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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