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Study · In vitro (cells) · mouse receptor · 1994

2 results

Molecular cloning, functional expression and pharmacological characterization of a mouse melanocortin receptor gene.

Desarnaud F, Labbe O, Eggerickx D, Vassart G, Parmentier M

The Biochemical journal1 Apr 1994Cited by 72 (Europe PMC)

PMID8172596 (opens in a new tab)PMCIDPMC1138281 (opens in a new tab)DOI10.1042/bj2990367 (opens in a new tab)PepBankPUB-08172596
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study2

2 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Activity measurement

    In CHO cells expressing the cloned mouse melanocortin receptor, iodinated NDP-MSH bound with an apparent Kd of 700 pM.

    In plain wordsIn cells carrying the cloned mouse receptor, NDP-MSH bound the receptor tightly, with an apparent Kd of 700 pM.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    Not applicableReceptor binding affinity (Kd)
    Study

    mouse receptorn: not reported in source

    CHO cells expressing the mouse melanocortin receptor
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Measurements

    Kd
    700 pM
    recombinant mouse receptor, CHO cells

    Source sentences

    Summary
    The recombinant receptor binds iodinated [Nle4,D-Phe7]alpha-MSH (NDP-MSH) with an apparent Kd of 700 pM.
    1 context sentence
    Summary · context
    Stable CHO cell lines expressing over 100,000 receptors per cell were generated.
  2. 02LabIn vitro (cells)Activity measurement

    In CHO cells expressing the cloned mouse receptor, NDP-MSH was the most powerful competitor for ligand binding, with an IC50 of 1.4 nM.

    In plain wordsIn cells carrying the cloned mouse receptor, NDP-MSH competed for binding more strongly than the other peptides tested, with an IC50 of 1.4 nM.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    Not applicableCompetition for receptor binding (IC50)
    Study

    mouse receptorn: not reported in source

    CHO cells expressing the mouse melanocortin receptor
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Measurements

    IC50
    1.4 nM
    recombinant mouse receptor, CHO cells

    Source sentences

    Summary
    NDP-MSH was the most powerful competitor (IC50 1.4 nM)
    1 context sentence
    Summary · context
    Stable CHO cell lines expressing over 100,000 receptors per cell were generated.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 21:44 UTC

    Study results updated

  2. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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