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Study · In vitro (cells) · mouse receptor in hamster cells · 1994

3 results

Molecular cloning of a mouse melanocortin 5 receptor gene widely expressed in peripheral tissues.

Labbé O, Desarnaud F, Eggerickx D, Vassart G, Parmentier M

Biochemistry1 Apr 1994Cited by 145 (Europe PMC)

PMID8161509 (opens in a new tab)DOI10.1021/bi00181a015 (opens in a new tab)PepBankPUB-08161509
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study3

3 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Activity measurement

    In CHO cells expressing the mouse MC5 receptor, radioiodinated NDP-MSH showed saturable binding with an apparent Kd of 1.47 +/- 0.15 nM.

    In plain wordsIn lab tests on cells with the mouse MC5 receptor, NDP-MSH bound the receptor strongly (Kd 1.47 nM).

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    Not applicableNDP-MSH saturable binding (Kd)
    Study

    mouse receptor in hamster cellsn: not reported in source

    stable CHO cell line expressing mouse melanocortin 5 receptor
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Measurements

    Kd
    1.47 nM
    CHO cells expressing MC5

    Source sentences

    Summary
    This cell line displayed a saturable binding capacity for the radioiodinated alpha-melanocyte-stimulating hormone (alpha-MSH) analog [Nle4,D-Phe7]-alpha-MSH (NDP-MSH) with an apparent Kd of 1.47 +/- 0.15 nM.
    1 context sentence
    Summary · context
    A stable Chinese hamster ovary (CHO) cell line expressing approximately 10,000 receptors per cell was established.
  2. 02LabIn vitro (cells)Activity measurement

    In CHO cells expressing the mouse MC5 receptor, NDP-MSH was the most powerful competitor for labeled ligand binding, followed by alpha-MSH, ACTH, beta-MSH, the gamma-MSHs, and ACTH 4-10.

    In plain wordsIn lab tests on cells, NDP-MSH competed for the MC5 receptor more strongly than the other melanocortin peptides tested.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    Not applicableNDP-MSH competition for binding
    Study

    mouse receptor in hamster cellsn: not reported in source

    stable CHO cell line expressing mouse melanocortin 5 receptor
    Comparator
    alpha-MSH, ACTH, beta-MSH, gamma-MSHs, ACTH 4-10
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    NDP-MSH was the most powerful competitor, followed by alpha-MSH, adrenocorticotropic hormone (ACTH), beta-MSH, the gamma-MSHs, and ACTH 4-10.
    1 context sentence
    Summary · context
    A stable Chinese hamster ovary (CHO) cell line expressing approximately 10,000 receptors per cell was established.
  3. 03LabIn vitro (cells)Activity measurement

    In CHO cells expressing the mouse MC5 receptor, the potency order of melanocortin peptides in cAMP accumulation assays was consistent with the binding order, in which NDP-MSH was the most powerful competitor.

    In plain wordsIn lab tests on cells, the peptides turned on the MC5 receptor signal in the same order as their binding, where NDP-MSH bound most strongly.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    Not applicablecAMP accumulation potency order
    Study

    mouse receptor in hamster cellsn: not reported in source

    stable CHO cell line expressing mouse melanocortin 5 receptor
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    NDP-MSH was the most powerful competitor, followed by alpha-MSH, adrenocorticotropic hormone (ACTH), beta-MSH, the gamma-MSHs, and ACTH 4-10. Functional assays confirmed that HGMP01B, like other melanocortin receptors, stimulated adenylyl cyclase. The potency order obtained in these cyclic adenosine…
    1 context sentence
    Summary · context
    A stable Chinese hamster ovary (CHO) cell line expressing approximately 10,000 receptors per cell was established.

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Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 21:44 UTC

    Study results updated

  2. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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