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Study · In vitro (cells) · 1994

6 results

Cultured human melanocytes respond to MSH peptides and ACTH.

Hunt G, Donatien PD, Lunec J, Todd C, Kyne S, Thody AJ

Pigment cell research1 Aug 1994Cited by 63 (Europe PMC)

PMID7855066 (opens in a new tab)DOI10.1111/j.1600-0749.1994.tb00052.x (opens in a new tab)PepBankPUB-07855066
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study6

6 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Efficacy outcome

    In cultured human melanocytes, NDP-MSH induced dose-related increases in melanin content.

    In plain wordsIn lab-grown human pigment cells, NDP-MSH raised melanin in a dose-related way.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    IncreaseMelanin content
    Study

    humann: not reported in source

    cultured human melanocytes
    Outcome group
    Pigmentation · Skin and wound
    Studied dose
    Dose not stated

    Source sentences

    Summary
    By culturing the cells in the absence of these additives, we demonstrate that alpha-MSH and its synthetic analogue Nle4DPhe7 alpha-MSH (NDP-MSH) induce dose-related increases in melanin content and tyrosinase activity and affect cell morphology in the majority of human melanocyte cultures.
  2. 02LabIn vitro (cells)Efficacy outcome

    In cultured human melanocytes, NDP-MSH induced dose-related increases in tyrosinase activity.

    In plain wordsIn lab-grown human pigment cells, NDP-MSH raised tyrosinase activity in a dose-related way.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    IncreaseTyrosinase activity
    Study

    humann: not reported in source

    cultured human melanocytes
    Outcome group
    Pigmentation · Skin and wound
    Studied dose
    Dose not stated

    Source sentences

    Summary
    By culturing the cells in the absence of these additives, we demonstrate that alpha-MSH and its synthetic analogue Nle4DPhe7 alpha-MSH (NDP-MSH) induce dose-related increases in melanin content and tyrosinase activity and affect cell morphology in the majority of human melanocyte cultures.
  3. 03LabIn vitro (cells)Other

    In cultured human melanocytes, NDP-MSH affected cell morphology in the majority of cultures.

    In plain wordsIn lab-grown human pigment cells, NDP-MSH changed how the cells looked in most cultures.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    Direction not reportedCell morphology
    Study

    humann: not reported in source

    cultured human melanocytes
    Outcome group
    Pigmentation · Skin and wound
    Studied dose
    Dose not stated

    Source sentences

    Summary
    By culturing the cells in the absence of these additives, we demonstrate that alpha-MSH and its synthetic analogue Nle4DPhe7 alpha-MSH (NDP-MSH) induce dose-related increases in melanin content and tyrosinase activity and affect cell morphology in the majority of human melanocyte cultures.
  4. 04LabIn vitro (cells)Mechanism

    In cultured human melanocytes, NDP-MSH increased tyrosinase mRNA.

    In plain wordsIn lab-grown human pigment cells, NDP-MSH raised tyrosinase mRNA.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    IncreaseTyrosinase mRNA
    Study

    humann: not reported in source

    cultured human melanocytes
    Outcome group
    Pigmentation · Skin and wound
    Studied dose
    Dose not stated

    Source sentences

    Summary
    In addition, NDP-MSH induces increases in tyrosinase mRNA and tyrosinase-related protein-1 (TRP-1) mRNA.
  5. 05LabIn vitro (cells)Mechanism

    In cultured human melanocytes, NDP-MSH increased tyrosinase-related protein-1 (TRP-1) mRNA.

    In plain wordsIn lab-grown human pigment cells, NDP-MSH raised TRP-1 mRNA.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    IncreaseTRP-1 mRNA
    Study

    humann: not reported in source

    cultured human melanocytes
    Outcome group
    Pigmentation · Skin and wound
    Studied dose
    Dose not stated

    Source sentences

    Summary
    In addition, NDP-MSH induces increases in tyrosinase mRNA and tyrosinase-related protein-1 (TRP-1) mRNA.
  6. 06LabIn vitro (cells)Activity measurement

    In cultured human melanocytes, the dose-response curves were sigmoidal and NDP-MSH and alpha-MSH were equipotent.

    In plain wordsIn lab-grown human pigment cells, NDP-MSH and alpha-MSH were equally potent.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    No significant changePotency versus alpha-MSH
    Study

    humann: not reported in source

    cultured human melanocytes
    Comparator
    alpha-MSH
    Outcome group
    Pigmentation · Skin and wound
    Studied dose
    Dose not stated

    Source sentences

    Summary
    The dose-response curves for the MSH peptides are sigmoidal and the two peptides are equipotent in their effects on human melanocytes.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 21:44 UTC

    Study results updated

  2. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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