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Study · Not a test of the peptide alone · 1983

4 results

In vitro synthesis of thymosin beta 4 encoded by rat spleen mRNA.

Filipowicz AW, Horecker BL

Proceedings of the National Academy of Sciences of the United States of America1 Apr 1983Cited by 13 (Europe PMC)

PMID6572941 (opens in a new tab)PMCIDPMC393699 (opens in a new tab)DOI10.1073/pnas.80.7.1811 (opens in a new tab)PepBankPUB-06572941
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study4

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone4

In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Thymosin beta-4 (human).

  1. 01LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cell-free)Mechanism

    Thymosin beta‑4 was synthesized in vitro in a rabbit reticulocyte lysate and a yeast protein-synthesis system from rat spleen mRNA, and the product was identified as thymosin beta‑4.

    In plain wordsIn lab tests, rat spleen mRNA produced thymosin beta‑4 in two cell-free protein-making systems, and tests identified the product.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    Not applicableIn vitro synthesis of thymosin beta 4 from rat spleen mRNA
    Study

    ratn: not reported in source

    cell-free translation systems with rat spleen mRNA
    Outcome group
    Peptide structure and chemistry · Pharmacology

    Source sentences

    Summary
    Thymosin beta 4, containing 43 amino acids and acetylated at the NH2 terminus, is synthesized in vitro in a rabbit reticulocyte lysate or in a yeast protein-synthesis system in the presence of mRNA from rat spleen.
    1 context sentence
    Summary · context
    The product formed was identified as beta 4 by immunoprecipitation by a specific anti-beta 4 antiserum, comigration with authentic beta 4 in NaDodSO4/polyacrylamide gel electrophoresis and in HPLC, and identity of peptide fragments.

    In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).

  2. 02LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cell-free)Mechanism

    In a wheat germ cell-free system, the immunoprecipitable product eluted slightly ahead of thymosin beta‑4 in HPLC and appeared to lack the NH2-terminal acetyl group.

    In plain wordsIn lab tests, the wheat germ system made a product that ran slightly ahead of thymosin beta‑4 and seemed to lack its acetyl group.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    Not applicableWheat germ system product versus authentic thymosin beta 4
    Study

    ratn: not reported in source

    cell-free translation systems with rat spleen mRNA
    Outcome group
    Peptide structure and chemistry · Pharmacology

    Source sentences

    Summary
    The immunoprecipitable product generated in the wheat germ protein-synthesizing system emerged slightly ahead of beta 4 in HPLC and appeared to lack the NH2-terminal acetyl group.

    In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).

  3. 03LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cell-free)Mechanism

    No larger polypeptide precursor of thymosin beta‑4 was found in any of the three cell-free systems.

    In plain wordsIn lab tests, none of the three systems showed a larger precursor of thymosin beta‑4.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    No significant changeLarger precursor polypeptide of thymosin beta 4
    Study

    ratn: not reported in source

    cell-free translation systems with rat spleen mRNA
    Outcome group
    Peptide structure and chemistry · Pharmacology

    Source sentences

    Summary
    There was no evidence for formation of a larger polypeptide precursor of beta 4 in any of the three systems used.

    In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).

  4. 04LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cell-free)Mechanism

    In sucrose density gradient centrifugation, the mRNA coding for thymosin beta‑4 was recovered in the 7-8S fraction.

    In plain wordsIn lab tests, the mRNA for thymosin beta‑4 was found in the 7-8S mRNA fraction.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    Not applicableSedimentation fraction of thymosin beta 4 mRNA
    Study

    ratn: not reported in source

    cell-free translation systems with rat spleen mRNA
    Outcome group
    Peptide structure and chemistry · Pharmacology

    Measurements

    mRNA sedimentation fraction
    7 to 8 S
    rat spleen mRNA

    Source sentences

    Summary
    In sucrose density gradient centrifugation, the mRNA coding for beta 4 was recovered in the 7-8S mRNA fraction.

    In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 01:52 UTC

    Study results updated

  2. Version 1

    12 Oct 2026, 18:17 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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