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Study · Ex vivo tissue · 1983

6 results

Biological assay of luteinizing hormone-releasing hormone (gonadorelin).

Buckingham JC, Cover PO

Journal of pharmacological methods1 Jun 1983

PMID6353073 (opens in a new tab)DOI10.1016/0160-5402(83)90063-3 (opens in a new tab)PepBankPUB-06353073
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study6

4 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabEx vivo tissueActivity measurement

    In vitro, columns of dispersed adenohypophysial cells were highly sensitive to LHRH-stimulated LH release.

    In plain wordsIn lab tests, columns of pituitary cells were highly sensitive to LHRH.

    Peptide
    Gonadorelin (GnRH, LHRH)
    Outcome
    Not applicableSensitivity of dispersed pituitary cell columns to LHRH
    Study

    n: not reported in source

    Dispersed pituitary cells and adenohypophysial tissue segments
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Columns of dispersed adenohypophysial cells were highly sensitive to LHRH.
    1 context sentence
    Summary · context
    The abilities of luteinizing hormone releasing hormone (LHRH) to stimulate the release of luteinizing hormone (LH) in vitro from dispersed pituitary cells or from segments of adenohypophysial tissue have both been exploited in an attempt to develop biological assays for its determination.
  2. 02LabEx vivo tissueActivity measurement

    In vitro, pituitary segments were less sensitive to LHRH than dispersed cells.

    In plain wordsIn lab tests, pituitary segments were less sensitive to LHRH than dispersed cells.

    Peptide
    Gonadorelin (GnRH, LHRH)
    Outcome
    Not applicableSensitivity of pituitary segments to LHRH
    Study

    n: not reported in source

    Dispersed pituitary cells and adenohypophysial tissue segments
    Comparator
    Dispersed pituitary cells
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Pituitary segments were less sensitive to LHRH than the dispersed cells.
    1 context sentence
    Summary · context
    The abilities of luteinizing hormone releasing hormone (LHRH) to stimulate the release of luteinizing hormone (LH) in vitro from dispersed pituitary cells or from segments of adenohypophysial tissue have both been exploited in an attempt to develop biological assays for its determination.
  3. 03LabEx vivo tissueActivity measurement

    In vitro, pretreatment (priming) with the releasing hormone increased the responsiveness of pituitary segments.

    In plain wordsIn lab tests, pretreating pituitary segments with the releasing hormone made them respond more.

    Peptide
    Gonadorelin (GnRH, LHRH)
    Outcome
    IncreaseEffect of priming on segment responsiveness
    Study

    n: not reported in source

    Dispersed pituitary cells and adenohypophysial tissue segments
    Comparator
    Unprimed segments
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones
    Studied dose
    Dose not stated

    Source sentences

    Summary
    However, their responsiveness was increased by pretreatment ('priming') with the releasing hormone.
    1 context sentence
    Summary · context
    The abilities of luteinizing hormone releasing hormone (LHRH) to stimulate the release of luteinizing hormone (LH) in vitro from dispersed pituitary cells or from segments of adenohypophysial tissue have both been exploited in an attempt to develop biological assays for its determination.
  4. 04LabEx vivo tissueActivity measurement

    In vitro, over a wide range of doses, LH released by primed segments was linearly related to the logarithm of the LHRH concentration.

    In plain wordsIn lab tests, LH released by primed pituitary segments rose in a straight line with the log of the LHRH concentration.

    Peptide
    Gonadorelin (GnRH, LHRH)
    Outcome
    Not applicableDose-response of LH release from primed segments
    Study

    n: not reported in source

    Dispersed pituitary cells and adenohypophysial tissue segments
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Over a wide range of doses, there was a linear relationship between the LH released by 'primed' segments and the logarithm of the concentration of LHRH.
    1 context sentence
    Summary · context
    The abilities of luteinizing hormone releasing hormone (LHRH) to stimulate the release of luteinizing hormone (LH) in vitro from dispersed pituitary cells or from segments of adenohypophysial tissue have both been exploited in an attempt to develop biological assays for its determination.

Studies that did not test the peptide alone2

In this study, Gonadorelin (GnRH, LHRH) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Gonadorelin (GnRH, LHRH).

  1. 05LabNot a test of Gonadorelin (GnRH, LHRH) aloneEx vivo tissueOther

    In the dispersed-cell system, LHRH responses appeared specific but varied markedly in size, so the system was not precise.

    In plain wordsIn lab tests, the cell system responded specifically to LHRH but was not precise as an assay.

    Peptide
    Gonadorelin (GnRH, LHRH)
    Outcome
    Not applicablePrecision of the dispersed-cell assay
    Study

    n: not reported in source

    Dispersed pituitary cells and adenohypophysial tissue segments
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones

    Source sentences

    Summary
    The responses appeared to be specific, but the marked continual variation in their magnitude rendered the system nonprecise.
    1 context sentence
    Summary · context
    The abilities of luteinizing hormone releasing hormone (LHRH) to stimulate the release of luteinizing hormone (LH) in vitro from dispersed pituitary cells or from segments of adenohypophysial tissue have both been exploited in an attempt to develop biological assays for its determination.

    In this study, Gonadorelin (GnRH, LHRH) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Gonadorelin (GnRH, LHRH).

  2. 06LabNot a test of Gonadorelin (GnRH, LHRH) aloneEx vivo tissueOther

    In vitro, the dose-response lines of hypothalamic extracts were parallel with those of the synthetic peptide.

    In plain wordsIn lab tests, hypothalamic extracts and the synthetic peptide gave parallel dose-response lines.

    Peptide
    Gonadorelin (GnRH, LHRH)
    Outcome
    Not applicableParallelism of hypothalamic extract and synthetic peptide dose-response
    Study

    n: not reported in source

    Dispersed pituitary cells and adenohypophysial tissue segments
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones

    Source sentences

    Summary
    The dose-response lines of hypothalamic extracts were parallel with those of the synthetic peptide.
    1 context sentence
    Summary · context
    The abilities of luteinizing hormone releasing hormone (LHRH) to stimulate the release of luteinizing hormone (LH) in vitro from dispersed pituitary cells or from segments of adenohypophysial tissue have both been exploited in an attempt to develop biological assays for its determination.

    In this study, Gonadorelin (GnRH, LHRH) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Gonadorelin (GnRH, LHRH).

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 21:44 UTC

    Study results updated

  2. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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