Cloning and sequence analysis of cDNA for rat spleen thymosin beta 4.

Wodnar-Filipowicz A, Gubler U, Furuichi Y, Richardson M, Nowoswiat EF, Poonian MS, Horecker BL

Proceedings of the National Academy of Sciences of the United States of America1 Apr 1984Cited by 22 (Europe PMC)

PMID6201851PMCIDPMC345045DOI10.1073/pnas.81.8.2295PepBankPUB-06201851

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study5

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone5

In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Thymosin beta-4 (human).

01LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cell-free)Mechanism
The cloned rat spleen cDNA has a methionyl initiator codon directly before the first seryl codon of mature thymosin beta‑4, which fits the absence of a signal peptide.
In plain wordsIn lab work, the rat gene for thymosin beta‑4 showed no signal peptide.
Peptide
Thymosin beta-4 (human)
Outcome
Not applicableInitiator codon and signal peptide
Study
ratn: not reported in source
cDNA of rat spleen thymosin beta 4
Outcome group
Peptide structure and chemistry · Pharmacology
Source sentences
Summary
The presence of a methionyl initiator codon immediately preceding the codon for the first seryl residue of mature thymosin beta 4 is consistent with previous results indicating the absence of a signal peptide in the product translated in vitro from rat spleen mRNA.
In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).
Report a problemFND-0620185101
02LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cell-free)Other
The cDNA sequence has two terminator codons right after the codon for the COOH-terminal seryl residue.
In plain wordsIn lab work, the rat thymosin beta‑4 gene sequence has two stop codons after the last amino acid.
Peptide
Thymosin beta-4 (human)
Outcome
Not applicableTerminator codons
Study
ratn: not reported in source
cDNA of rat spleen thymosin beta 4
Outcome group
Peptide structure and chemistry · Pharmacology
Source sentences
Summary
The cDNA sequence analysis also established the presence of two terminator codons immediately following the codon for the COOH-terminal seryl residue.
In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).
Report a problemFND-0620185102
03LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cell-free)Other
Thymosin beta‑4 is synthesized as a 5100-dalton peptide with 44 amino acid residues.
In plain wordsIn lab work, thymosin beta‑4 is made as a peptide of 44 amino acids.
Peptide
Thymosin beta-4 (human)
Outcome
Not applicableSize of the synthesized peptide
Study
ratn: not reported in source
cDNA of rat spleen thymosin beta 4
Outcome group
Peptide structure and chemistry · Pharmacology
Measurements
Molecular mass
5100 dalton
synthesized peptide
Length
44 amino acid residues
synthesized peptide
Source sentences
Summary
Thymosin beta 4 is thus synthesized as a 5100-dalton peptide containing 44 amino acid residues.
In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).
Report a problemFND-0620185103
04LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cell-free)Other
Removal of the initiator methionine and acetylation of the NH2-terminal serine would give mature thymosin beta‑4 with 43 amino acids.
In plain wordsIn lab work, the mature thymosin beta‑4 is predicted to have 43 amino acids.
Peptide
Thymosin beta-4 (human)
Outcome
Not applicableMature peptide length
Study
ratn: not reported in source
cDNA of rat spleen thymosin beta 4
Outcome group
Peptide structure and chemistry · Pharmacology
Measurements
Length
43 amino acids
mature peptide
Source sentences
Summary
Removal of the initiator methionyl residue and acetylation of the NH2-terminal serine residue would yield mature thymosin beta 4 containing 43 amino acids.
In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).
Report a problemFND-0620185104
05LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cell-free)Mechanism
The absence of a signal peptide makes it unlikely that thymosin beta‑4 is a secreted peptide.
In plain wordsIn lab work, no signal peptide was found, so thymosin beta‑4 is unlikely to be released from cells.
Peptide
Thymosin beta-4 (human)
Outcome
Not applicableSecretion of thymosin beta 4
Study
ratn: not reported in source
cDNA of rat spleen thymosin beta 4
Outcome group
Peptide structure and chemistry · Pharmacology
Source sentences
Summary
The absence of a signal peptide makes it unlikely that thymosin beta 4 is a secreted peptide.
In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).
Report a problemFND-0620185105

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
13 Oct 2026, 01:52 UTC
Study results updated
Version 1
12 Oct 2026, 18:17 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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MeSH terms

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