Covalent linking of poly(ethyleneglycol)-bound NAD with Thermus thermophilus malate dehydrogenase. NAD(H)-regeneration unit for a coupled second-enzyme reaction.

Eguchi T, Iizuka T, Kagotani T, Lee JH, Urabe I, Okada H

European journal of biochemistry1 Mar 1986Cited by 11 (Europe PMC)

PMID3956494DOI10.1111/j.1432-1033.1986.tb09507.xPepBankPUB-03956494

Publisher page PubMedAsk about this study

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study11

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone11

In this study, NAD+ (nicotinamide adenine dinucleotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking NAD+ (nicotinamide adenine dinucleotide).

01LabNot a test of NAD+ (nicotinamide adenine dinucleotide) aloneIn vitro (cell-free)Activity measurement
In lab tests, MDH-PEG-NAD I contained an average of 1.2 NAD moieties per enzyme subunit.
In plain wordsIn lab tests, preparation I contained an average of 1.2 NAD units per enzyme subunit.
Peptide
NAD+ (nicotinamide adenine dinucleotide)
Outcome
Not applicableNAD moieties per subunit in preparation I
Study
Thermus thermophilusn: not reported in source
malate-dehydrogenase--PEG--NAD complex
Outcome group
Receptor activity and mechanism · Pharmacology
Measurements
NAD moieties per subunit
1.2 moieties/subunit
MDH-PEG-NAD I
Source sentences
Summary
The average numbers of NAD moieties covalently bound per subunit of MDH-PEG-NAD I, MDH-PEG-NAD II, MDH-PEG-NAD III and MDH-PEG-NAD IV were 1.2, 1.2, 0.8 and 0.5, respectively, and the values were confirmed by sodium dodecyl sulfate/polyacrylamide gel electrophoresis.
In this study, NAD+ (nicotinamide adenine dinucleotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking NAD+ (nicotinamide adenine dinucleotide).
Report a problemFND-0395649401
02LabNot a test of NAD+ (nicotinamide adenine dinucleotide) aloneIn vitro (cell-free)Activity measurement
In lab tests, MDH-PEG-NAD II contained an average of 1.2 NAD moieties per enzyme subunit.
In plain wordsIn lab tests, preparation II contained an average of 1.2 NAD units per enzyme subunit.
Peptide
NAD+ (nicotinamide adenine dinucleotide)
Outcome
Not applicableNAD moieties per subunit in preparation II
Study
Thermus thermophilusn: not reported in source
malate-dehydrogenase--PEG--NAD complex
Outcome group
Receptor activity and mechanism · Pharmacology
Measurements
NAD moieties per subunit
1.2 moieties/subunit
MDH-PEG-NAD II
Source sentences
Summary
The average numbers of NAD moieties covalently bound per subunit of MDH-PEG-NAD I, MDH-PEG-NAD II, MDH-PEG-NAD III and MDH-PEG-NAD IV were 1.2, 1.2, 0.8 and 0.5, respectively, and the values were confirmed by sodium dodecyl sulfate/polyacrylamide gel electrophoresis.
In this study, NAD+ (nicotinamide adenine dinucleotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking NAD+ (nicotinamide adenine dinucleotide).
Report a problemFND-0395649402
03LabNot a test of NAD+ (nicotinamide adenine dinucleotide) aloneIn vitro (cell-free)Activity measurement
In lab tests, MDH-PEG-NAD III contained an average of 0.8 NAD moieties per enzyme subunit.
In plain wordsIn lab tests, preparation III contained an average of 0.8 NAD units per enzyme subunit.
Peptide
NAD+ (nicotinamide adenine dinucleotide)
Outcome
Not applicableNAD moieties per subunit in preparation III
Study
Thermus thermophilusn: not reported in source
malate-dehydrogenase--PEG--NAD complex
Outcome group
Receptor activity and mechanism · Pharmacology
Measurements
NAD moieties per subunit
0.8 moieties/subunit
MDH-PEG-NAD III
Source sentences
Summary
The average numbers of NAD moieties covalently bound per subunit of MDH-PEG-NAD I, MDH-PEG-NAD II, MDH-PEG-NAD III and MDH-PEG-NAD IV were 1.2, 1.2, 0.8 and 0.5, respectively, and the values were confirmed by sodium dodecyl sulfate/polyacrylamide gel electrophoresis.
In this study, NAD+ (nicotinamide adenine dinucleotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking NAD+ (nicotinamide adenine dinucleotide).
Report a problemFND-0395649403
04LabNot a test of NAD+ (nicotinamide adenine dinucleotide) aloneIn vitro (cell-free)Activity measurement
In lab tests, MDH-PEG-NAD IV contained an average of 0.5 NAD moieties per enzyme subunit.
In plain wordsIn lab tests, preparation IV contained an average of 0.5 NAD units per enzyme subunit.
Peptide
NAD+ (nicotinamide adenine dinucleotide)
Outcome
Not applicableNAD moieties per subunit in preparation IV
Study
Thermus thermophilusn: not reported in source
malate-dehydrogenase--PEG--NAD complex
Outcome group
Receptor activity and mechanism · Pharmacology
Measurements
NAD moieties per subunit
0.5 moieties/subunit
MDH-PEG-NAD IV
Source sentences
Summary
The average numbers of NAD moieties covalently bound per subunit of MDH-PEG-NAD I, MDH-PEG-NAD II, MDH-PEG-NAD III and MDH-PEG-NAD IV were 1.2, 1.2, 0.8 and 0.5, respectively, and the values were confirmed by sodium dodecyl sulfate/polyacrylamide gel electrophoresis.
In this study, NAD+ (nicotinamide adenine dinucleotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking NAD+ (nicotinamide adenine dinucleotide).
Report a problemFND-0395649404
05LabNot a test of NAD+ (nicotinamide adenine dinucleotide) aloneIn vitro (cell-free)Activity measurement
In lab tests, the enzyme moiety reduced 60-80% of bound NAD in MDH-PEG-NAD preparations with L-malate.
In plain wordsIn lab tests, the enzyme reduced 60-80% of bound NAD in the complexes with L-malate.
Peptide
NAD+ (nicotinamide adenine dinucleotide)
Outcome
Not applicableReduction of bound NAD
Study
Thermus thermophilusn: not reported in source
malate-dehydrogenase--PEG--NAD complex
Outcome group
Receptor activity and mechanism · Pharmacology
Measurements
Bound NAD reduced
60 to 80%
with L-malate
Source sentences
Summary
60-80% bound NAD moiety of these preparations of MDH-PEG-NAD was reduced by the enzyme moiety in the presence of L-malate, and the specific activity of the enzyme moiety of the preparations was more than 80% that of the native enzyme.
In this study, NAD+ (nicotinamide adenine dinucleotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking NAD+ (nicotinamide adenine dinucleotide).
Report a problemFND-0395649405
06LabNot a test of NAD+ (nicotinamide adenine dinucleotide) aloneIn vitro (cell-free)Activity measurement
In lab tests, MDH-PEG-NAD preparations retained more than 80% of native enzyme specific activity.
In plain wordsIn lab tests, the NAD complexes retained more than 80% of the original enzyme activity.
Peptide
NAD+ (nicotinamide adenine dinucleotide)
Outcome
Not applicableEnzyme specific activity
Study
Thermus thermophilusn: not reported in source
malate-dehydrogenase--PEG--NAD complex
Comparator
native enzyme
Outcome group
Receptor activity and mechanism · Pharmacology
Source sentences
Summary
60-80% bound NAD moiety of these preparations of MDH-PEG-NAD was reduced by the enzyme moiety in the presence of L-malate, and the specific activity of the enzyme moiety of the preparations was more than 80% that of the native enzyme.
In this study, NAD+ (nicotinamide adenine dinucleotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking NAD+ (nicotinamide adenine dinucleotide).
Report a problemFND-0395649406
07LabNot a test of NAD+ (nicotinamide adenine dinucleotide) aloneIn vitro (cell-free)Activity measurement
In lab tests, the Km for exogenous NAD in MDH-PEG-NAD I was three times the native enzyme value.
In plain wordsIn lab tests, the complex had a higher Km for NAD than the original enzyme.
Peptide
NAD+ (nicotinamide adenine dinucleotide)
Outcome
IncreaseKm for exogenous NAD
Study
Thermus thermophilusn: not reported in source
malate-dehydrogenase--PEG--NAD complex
Comparator
native enzyme
Outcome group
Receptor activity and mechanism · Pharmacology
Source sentences
Summary
The Km value for exogenous NAD is three times that of the native enzyme.
In this study, NAD+ (nicotinamide adenine dinucleotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking NAD+ (nicotinamide adenine dinucleotide).
Report a problemFND-0395649407
08LabNot a test of NAD+ (nicotinamide adenine dinucleotide) aloneIn vitro (cell-free)Activity measurement
In lab tests, the NAD moiety of MDH-PEG-NAD I had 20-40% of native NAD coenzyme activity for alcohol dehydrogenase.
In plain wordsIn lab tests, complex-bound NAD had lower activity with alcohol dehydrogenase than native NAD.
Peptide
NAD+ (nicotinamide adenine dinucleotide)
Outcome
DecreaseCoenzyme activity with alcohol dehydrogenase
Study
Thermus thermophilusn: not reported in source
malate-dehydrogenase--PEG--NAD complex
Comparator
native NAD
Outcome group
Receptor activity and mechanism · Pharmacology
Source sentences
Summary
The coenzyme activity of its NAD moiety is 20-40% that of native NAD for alcohol and lactate dehydrogenases.
In this study, NAD+ (nicotinamide adenine dinucleotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking NAD+ (nicotinamide adenine dinucleotide).
Report a problemFND-0395649408
09LabNot a test of NAD+ (nicotinamide adenine dinucleotide) aloneIn vitro (cell-free)Activity measurement
In lab tests, the NAD moiety of MDH-PEG-NAD I had 20-40% of native NAD coenzyme activity for lactate dehydrogenase.
In plain wordsIn lab tests, complex-bound NAD had lower activity with lactate dehydrogenase than native NAD.
Peptide
NAD+ (nicotinamide adenine dinucleotide)
Outcome
DecreaseCoenzyme activity with lactate dehydrogenase
Study
Thermus thermophilusn: not reported in source
malate-dehydrogenase--PEG--NAD complex
Comparator
native NAD
Outcome group
Receptor activity and mechanism · Pharmacology
Source sentences
Summary
The coenzyme activity of its NAD moiety is 20-40% that of native NAD for alcohol and lactate dehydrogenases.
In this study, NAD+ (nicotinamide adenine dinucleotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking NAD+ (nicotinamide adenine dinucleotide).
Report a problemFND-0395649409
10LabNot a test of NAD+ (nicotinamide adenine dinucleotide) aloneIn vitro (cell-free)Activity measurement
In lab tests, MDH-PEG-NAD I catalyzed L-malate oxidation with a redox system at a rate constant of 0.11 s-1.
In plain wordsIn lab tests, the NAD complex catalyzed L-malate oxidation with the tested redox system.
Peptide
NAD+ (nicotinamide adenine dinucleotide)
Outcome
Not applicableL-malate oxidation rate constant
Study
Thermus thermophilusn: not reported in source
malate-dehydrogenase--PEG--NAD complex
Outcome group
Receptor activity and mechanism · Pharmacology
Measurements
Rate constant
0.11 s-1
L-malate oxidation
Source sentences
Summary
The complex catalyzes the oxidation of L-malate in the presence of the redox system of 5-ethylphenazinium ethyl sulfate and a tetrazolium salt with a rate constant of 0.11 s-1.
In this study, NAD+ (nicotinamide adenine dinucleotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking NAD+ (nicotinamide adenine dinucleotide).
Report a problemFND-0395649410
11LabNot a test of NAD+ (nicotinamide adenine dinucleotide) aloneIn vitro (cell-free)Mechanism
In lab tests, coupled reactions of MDH-PEG-NAD I and alcohol dehydrogenase recycled the complex's coenzyme moiety.
In plain wordsIn lab tests, coupled enzyme reactions recycled NAD within the complex.
Peptide
NAD+ (nicotinamide adenine dinucleotide)
Outcome
Not applicableCoenzyme recycling
Study
Thermus thermophilusn: not reported in source
malate-dehydrogenase--PEG--NAD complex
Outcome group
Receptor activity and mechanism · Pharmacology
Source sentences
Summary
The coenzyme moiety of the complex can also be recycled by coupled reactions of the active site of the same complex and alcohol dehydrogenase.
In this study, NAD+ (nicotinamide adenine dinucleotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking NAD+ (nicotinamide adenine dinucleotide).
Report a problemFND-0395649411

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
14 Oct 2026, 05:02 UTC
Study results updated
Version 1
14 Oct 2026, 01:46 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

Publication types

MeSH terms

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