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Study · Animal study (in vivo) · mouse and rat · 1987

4 results

Specific receptors for alpha-melanocyte-stimulating hormone are widely distributed in tissues of rodents.

Tatro JB, Reichlin S

Endocrinology1 Nov 1987Cited by 96 (Europe PMC)

PMID2822378 (opens in a new tab)DOI10.1210/endo-121-5-1900 (opens in a new tab)PepBankPUB-02822378
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study4

4 results from studies that gave the peptide. No result from this study comes from people.

  1. 01AnimalAnimal study (in vivo)Pharmacokinetics

    In mice given [125I]NDP-MSH intravenously, specific binding was found in many glandular organs, adipose tissues, bladder, duodenum, skin, spleen and hypothalamus.

    In plain wordsIn mice, NDP-MSH given into a vein bound specifically in many organs, including glands, fat, skin and the hypothalamus.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    Not applicableTissue distribution of specific binding in mice
    Study

    mousen: not reported in source

    C3H/HeJ pigmented mice
    Outcome group
    Pharmacokinetics · Pharmacology
    Studied dose
    Dose not stated · intravenousAs given in this study (mouse).

    Source sentences

    Summary
    In mice, specific (i.e. alpha MSH-inhibitable) binding of [125I]NDP-MSH was found in a number of glandular organs, including lacrimal, Harderian, preputial, submandibular, and adrenal glands and pancreas, as well as in brown and white adipose tissues, bladder, duodenum, skin, spleen, and hypothalamus.
    1 context sentence
    Summary · context
    To study receptor distribution in vivo, [125I]NDP-MSH was administered iv to C3H/HeJ (pigmented) mice and Sprague-Dawley (albino) rats.
  2. 02AnimalAnimal study (in vivo)Pharmacokinetics

    In rats given [125I]NDP-MSH intravenously, specific tracer uptake was observed in lacrimal, Harderian, preputial and thyroid glands, pancreas, duodenum, spleen, hypothalamus and white adipose tissue.

    In plain wordsIn rats, NDP-MSH given into a vein was taken up specifically in several glands, the gut, spleen, hypothalamus and fat.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    Not applicableTissue distribution of specific binding in rats
    Study

    ratn: not reported in source

    Sprague-Dawley albino rats
    Outcome group
    Pharmacokinetics · Pharmacology
    Studied dose
    Dose not stated · intravenousAs given in this study (rat).

    Source sentences

    Summary
    In rats, results were generally similar; specific tracer uptake was observed in lacrimal, Harderian, preputial, and thyroid glands; pancreas, duodenum, spleen, hypothalamus; and white adipose tissue.
    1 context sentence
    Summary · context
    To study receptor distribution in vivo, [125I]NDP-MSH was administered iv to C3H/HeJ (pigmented) mice and Sprague-Dawley (albino) rats.
  3. 03LabIn vitro (cells)Activity measurement

    In cultured B16 and Cloudman S91 mouse melanoma cells, [125I]NDP-MSH showed consistent and specific binding.

    In plain wordsIn mouse melanoma cells in the lab, NDP-MSH bound consistently and specifically.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    Not applicableBinding to cultured melanoma cells
    Study

    mousen: not reported in source

    cultured B16 and Cloudman S91 murine melanoma cells
    Outcome group
    Receptor activity and mechanism · Cancer
    Studied dose
    Dose not stated

    Source sentences

    Summary
    [125I]NDP-MSH exhibited consistent and specific binding to cultured B16 and Cloudman S91 murine melanoma cells, which are highly responsive to alpha MSH.
    1 context sentence
    Summary · context
    The superpotent and enzymatically resistant alpha MSH analog, Nle4,D-Phe7-alpha MSH (NDP-MSH), was radioiodinated using lactoperoxidase (Enzymobeads) and purified by reverse phase chromatography for use as a tracer.
  4. 04LabIn vitro (cells)Activity measurement

    In B16 mouse melanoma cells, the radioiodinated NDP-MSH tracer had full biological activity in stimulating melanogenesis.

    In plain wordsIn mouse melanoma cells in the lab, labelled NDP-MSH fully stimulated pigment production.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    IncreaseMelanogenesis in B16 cells
    Study

    mousen: not reported in source

    B16 mouse melanoma cells
    Outcome group
    Pigmentation · Skin and wound
    Studied dose
    Dose not stated

    Source sentences

    Summary
    The tracer had full biological activity, as determined by its potency in stimulating melanogenesis in B16 cells.
    1 context sentence
    Summary · context
    The superpotent and enzymatically resistant alpha MSH analog, Nle4,D-Phe7-alpha MSH (NDP-MSH), was radioiodinated using lactoperoxidase (Enzymobeads) and purified by reverse phase chromatography for use as a tracer.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 21:44 UTC

    Study results updated

  2. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

Subjects

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