Thymosin-beta 4 gene. Preliminary characterization and expression in tissues, thymic cells, and lymphocytes.

Gómez-Márquez J, Dosil M, Segade F, Bustelo XR, Pichel JG, Dominguez F, Freire M

Journal of immunology (Baltimore, Md. : 1950)1 Oct 1989Cited by 27 (Europe PMC)

PMID2677145DOI10.4049/jimmunol.143.8.2740PepBankPUB-02677145

Publisher page PubMedAsk about this study

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study6

No result from this study comes from a test of the peptide.

Natural levels measured4

These results measure the body's own peptide. Nobody was given the peptide. They are not evidence about taking it. They are left out of key results, evidence counts and charts.

01AnimalEndogenous levelAnimal study (in vivo)Mechanism
In rat tissues, an 800 nucleotide thymosin beta‑4 mRNA was present in all 13 tissues examined, with the highest levels in spleen, thymus and lung.
In plain wordsIn rats, the body's own thymosin beta‑4 messenger RNA was found in all tissues tested, most in spleen, thymus and lung.
Peptide
Thymosin beta-4 (human)
Outcome
Not applicableThymosin-beta 4 mRNA across 13 rat tissues
Study
ratn: not reported in source
Outcome group
Gene expression · Whole body
Measurements
mRNA length
800 nucleotides
rat tissues
Source sentences
Summary
Hybridization analysis of total RNA from 13 rat tissues demonstrated the presence of an 800 nucleotides-long mRNA in all the tissues surveyed, with the highest levels in spleen, thymus, and lung.
1 context sentence(design, population, supporting numbers)
Summary · context
A cDNA for rat thymosin-beta 4 was used to investigate the expression of this gene in different tissues, thymic cells, and lymphocytes.
This result measures the body's own Thymosin beta-4 (human). Nobody was given the peptide. It is not evidence about taking it.
Report a problemFND-0267714501
02AnimalEndogenous levelAnimal study (in vivo)Mechanism
In rat thymic cells, thymosin beta‑4 gene expression was predominantly in thymocytes.
In plain wordsIn rats, the body's own thymosin beta‑4 gene was active mainly in thymocytes.
Peptide
Thymosin beta-4 (human)
Outcome
Not applicableThymosin-beta 4 mRNA in thymic cells
Study
ratn: not reported in source
Outcome group
Gene expression · Immune and inflammation
Source sentences
Summary
Examination of thymic cells showed that the thymosin-beta 4 gene is predominantly expressed in thymocytes.
1 context sentence(design, population, supporting numbers)
Summary · context
A cDNA for rat thymosin-beta 4 was used to investigate the expression of this gene in different tissues, thymic cells, and lymphocytes.
This result measures the body's own Thymosin beta-4 (human). Nobody was given the peptide. It is not evidence about taking it.
Report a problemFND-0267714502
03AnimalEndogenous levelAnimal study (in vivo)Mechanism
Thymosin beta‑4 mRNA was fourfold more abundant in Ig- than Ig+ splenic lymphocytes, while blood cells of both types had similar levels.
In plain wordsIn rats, the body's own thymosin beta‑4 messenger RNA was higher in one type of spleen lymphocyte, but similar in the two blood cell types.
Peptide
Thymosin beta-4 (human)
Outcome
MixedThymosin-beta 4 mRNA in Ig+ and Ig- lymphocytes
Study
ratn: not reported in source
Comparator
Ig+ lymphocytes
Outcome group
Gene expression · Immune and inflammation
Source sentences
Summary
The thymosin-beta 4 mRNA was also studied in Ig+ and Ig- lymphocytes, being fourfold more abundant in Ig- than Ig+ splenic lymphocytes, whereas similar levels were found in both types of blood cells.
1 context sentence(design, population, supporting numbers)
Summary · context
A cDNA for rat thymosin-beta 4 was used to investigate the expression of this gene in different tissues, thymic cells, and lymphocytes.
This result measures the body's own Thymosin beta-4 (human). Nobody was given the peptide. It is not evidence about taking it.
Report a problemFND-0267714503
04AnimalEndogenous levelAnimal study (in vivo)Mechanism
Thymosin beta‑4 mRNA content differed only slightly between T cells at different maturation stages.
In plain wordsIn rats, the body's own thymosin beta‑4 messenger RNA changed only slightly as T cells matured.
Peptide
Thymosin beta-4 (human)
Outcome
No significant changeThymosin-beta 4 mRNA across T cell maturation stages
Study
ratn: not reported in source
Outcome group
Gene expression · Immune and inflammation
Source sentences
Summary
The analysis of RNA from T cells at different maturation stages evidenced slight differences in their thymosin-beta 4 mRNA content, indicating that thymosin-beta 4 gene expression is not clearly related to the differentiation process of T cells.
1 context sentence(design, population, supporting numbers)
Summary · context
A cDNA for rat thymosin-beta 4 was used to investigate the expression of this gene in different tissues, thymic cells, and lymphocytes.
This result measures the body's own Thymosin beta-4 (human). Nobody was given the peptide. It is not evidence about taking it.
Report a problemFND-0267714504

Studies that did not test the peptide alone2

In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Thymosin beta-4 (human).

05LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cell-free)Other
Analysis of genomic DNA from species ranging from humans to Escherichia coli showed that the thymosin beta‑4 gene is highly conserved only in mammals.
In plain wordsIn lab tests, the thymosin beta‑4 gene was highly conserved only in mammals among the species tested.
Peptide
Thymosin beta-4 (human)
Outcome
Not applicableConservation of the thymosin-beta 4 gene across species
Study
n: not reported in source
genomic DNA hybridization
Outcome group
Gene expression · Whole body
Source sentences
Summary
The analysis of genomic DNA from different species ranging from humans to Escherichia coli showed that this gene is only highly conserved in mammals.
1 context sentence(design, population, supporting numbers)
Summary · context
A cDNA for rat thymosin-beta 4 was used to investigate the expression of this gene in different tissues, thymic cells, and lymphocytes.
In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).
Report a problemFND-0267714505
06LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cell-free)Other
Restriction analysis of the human thymosin beta‑4 gene gave a complicated pattern consistent with multiple genes and/or introns.
In plain wordsIn lab tests, the human thymosin beta‑4 gene gave a complicated pattern that may mean several genes or introns.
Peptide
Thymosin beta-4 (human)
Outcome
Not applicableRestriction pattern of the human thymosin-beta 4 gene
Study
humann: not reported in source
restriction analysis of genomic DNA
Outcome group
Synthesis and manufacturing · Pharmacology
Source sentences
Summary
Preliminary characterization of the human beta 4 gene by restriction analysis disclosed a complicated pattern consistent with multiple genes and/or introns.
In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).
Report a problemFND-0267714506

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
13 Oct 2026, 01:52 UTC
Study results updated
Version 1
12 Oct 2026, 18:17 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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MeSH terms

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