Skip to content

Study · Not a test of the peptide alone · 1989

3 results

Production in Escherichia coli of human thymosin beta 4 as chimeric protein with human tumor necrosis factor.

Tsuji Y, Kitahara-Tanabe N, Noguchi K, Gatanaga T, Mizuno D, Soma G

Biochemistry international1 Mar 1989Cited by 3 (Europe PMC)

PMID2669759 (opens in a new tab)PepBankPUB-02669759
Publisher pageAsk about this study
Jump to 3 results

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study3

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone3

In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Thymosin beta-4 (human).

  1. 01LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cells)Other

    In E. coli, a chimeric protein of thymosin beta‑4 and TNF was abundantly expressed and reacted with both anti-thymosin beta‑4 and anti-TNF antibodies.

    In plain wordsIn lab tests, bacteria made large amounts of a fusion protein of thymosin beta‑4 and TNF that antibodies to both parts recognised.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    Not applicableExpression of thymosin beta 4-TNF chimeric protein in E. coli
    Study

    Escherichia colin: not reported in source

    E. coli expression system
    Outcome group
    Synthesis and manufacturing · Pharmacology

    Source sentences

    Summary
    The protein was abundantly expressed, was immunoreactive against both anti-thymosin beta 4 and anti-TNF antibodies, and retained cytotoxicity in a TNF assay using mouse L929 fibroblasts.
    1 context sentence
    Summary · context
    We have produced thymosin beta 4 protein in Escherichia coli as a chimeric protein with tumor necrosis factor (TNF).

    In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).

  2. 02LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cells)Activity measurement

    The thymosin beta‑4-TNF chimeric protein kept cytotoxicity in a TNF assay with mouse L929 fibroblasts.

    In plain wordsIn cells, the fusion protein of thymosin beta‑4 and TNF kept the cell-killing activity of TNF.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    Not applicableTNF cytotoxicity of the chimeric protein
    Study

    mousen: not reported in source

    L929 fibroblasts
    Outcome group
    Receptor activity and mechanism · Pharmacology

    Source sentences

    Summary
    The protein was abundantly expressed, was immunoreactive against both anti-thymosin beta 4 and anti-TNF antibodies, and retained cytotoxicity in a TNF assay using mouse L929 fibroblasts.
    1 context sentence
    Summary · context
    We have produced thymosin beta 4 protein in Escherichia coli as a chimeric protein with tumor necrosis factor (TNF).

    In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).

  3. 03LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cells)Other

    The TNF activity of the chimeric protein allowed simple purification of thymosin beta‑4 by following that activity.

    In plain wordsIn lab tests, the TNF activity of the fusion protein made it easy to purify thymosin beta‑4.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    Not applicablePurification of thymosin beta 4 by TNF activity
    Study

    Escherichia colin: not reported in source

    E. coli expression system
    Outcome group
    Synthesis and manufacturing · Pharmacology

    Source sentences

    Summary
    This latter characteristic enabled the easy and simple purification of thymosin beta 4 merely by following the TNF activity.
    1 context sentence
    Summary · context
    We have produced thymosin beta 4 protein in Escherichia coli as a chimeric protein with tumor necrosis factor (TNF).

    In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 01:52 UTC

    Study results updated

  2. Version 1

    12 Oct 2026, 18:17 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

Publication types

PepBank is a research reference. Talk to a doctor before you use any peptide.