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Study · In vitro (cells) · rat · 1989

2 results

Morphology and function of lacrimal gland acinar cells in primary culture.

Hann LE, Tatro JB, Sullivan DA

Investigative ophthalmology & visual science1 Jan 1989Cited by 53 (Europe PMC)

PMID2536359 (opens in a new tab)PepBankPUB-02536359
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study2

2 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Activity measurement

    In freshly isolated rat lacrimal acinar cells, labelled Nle4-D-Phe7-alpha-MSH showed specific binding sites.

    In plain wordsIn rat tear gland cells in the lab, labelled NDP-MSH bound to specific sites on the cells.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    Not applicableSpecific binding sites on isolated acinar cells
    Study

    ratn: not reported in source

    freshly isolated rat lacrimal gland acinar cells
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones
    Studied dose
    Dose not stated

    Source sentences

    Summary
    In addition, experiments with a labeled analog (Nle4-D-Phe7-alpha MSH) of alpha-melanocyte-stimulating hormone (alpha-MSH) demonstrated specific binding sites on freshly isolated cells; alpha-MSH is a known modulator of acinar protein secretion.
    1 context sentence
    Summary · context
    Acinar cells were isolated by serial incubation of gland fragments in chelating and enzymatic solutions, followed by centrifugation through a Ficoll gradient.
  2. 02LabIn vitro (cells)Activity measurement

    In rat lacrimal acinar cells, maximum binding of the alpha-MSH analog occurred within 30 minutes, depended on cell density and was reduced by unlabeled alpha-MSH.

    In plain wordsIn rat tear gland cells in the lab, NDP-MSH binding peaked within 30 minutes and was reduced when unlabelled alpha-MSH was present.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    Not applicableTime course, cell-density dependence and displacement of binding
    Study

    ratn: not reported in source

    freshly isolated rat lacrimal gland acinar cells
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones
    Studied dose
    Dose not stated

    Measurements

    Time to maximum binding
    ≤ 30 min
    isolated acinar cells

    Source sentences

    Summary
    Maximum binding of the alpha-MSH analog occurred within 30 min, was dependent upon cell density and was reduced by coincubation with unlabeled alpha-MSH.
    2 context sentences
    Summary · context
    In addition, experiments with a labeled analog (Nle4-D-Phe7-alpha MSH) of alpha-melanocyte-stimulating hormone (alpha-MSH) demonstrated specific binding sites on freshly isolated cells; alpha-MSH is a known modulator of acinar protein secretion.
    Summary · context
    Acinar cells were isolated by serial incubation of gland fragments in chelating and enzymatic solutions, followed by centrifugation through a Ficoll gradient.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 21:44 UTC

    Study results updated

  2. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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