Rapid induction of thymosin beta 4 in concanavalin A-stimulated thymocytes by translational control.

Schöbitz B, Netzker R, Hannappel E, Brand K

The Journal of biological chemistry1 Sept 1990Cited by 24 (Europe PMC)

PMID2394730DOI10.1016/s0021-9258(18)55408-9PepBankPUB-02394730

Publisher page PubMedAsk about this study

Summary

The expression of thymosin beta 4, an ubiquitous peptide of high cellular content, was studied in concanavalin A-stimulated rat thymocytes within the first 3 h after activation of the cells. An early 6.3-fold increase of the peptide occurred after 1 h of stimulation amounting to 0.4% of the total cellular protein. This increase coincided with that of thymosin beta 4 biosynthesis measured by [35S]methionine incorporation. The share of thymosin beta 4 synthesis in total protein synthesis 1 h after addition of concanavalin A amounts to 1% but no elevation of the corresponding mRNA was observed. These data suggest that a translational control mechanism is involved in this rapid induction. Consequently, actinomycin D did not inhibit thymosin beta 4 induction in contrast to cycloheximide. The peaks of maximal thymosin beta 4 levels and biosynthesis were followed by rapid decreases of these parameters suggesting a function of thymosin beta 4 in the early phase of T cell activation.

CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study5

No result from this study comes from a test of the peptide.

Natural levels measured5

These results measure the body's own peptide. Nobody was given the peptide. They are not evidence about taking it. They are left out of key results, evidence counts and charts.

01LabEndogenous levelIn vitro (cells)Mechanism
In concanavalin A-stimulated rat thymocytes, thymosin beta‑4 content rose 6.3-fold after 1 h, reaching 0.4% of total cellular protein.
In plain wordsIn rat immune cells, the body's own thymosin beta‑4 rose sharply within 1 hour of stimulation with concanavalin A.
Peptide
Thymosin beta-4 (human)
Outcome
IncreaseTβ4 content 1 h after ConA
Study
ratn: not reported in source
concanavalin A-stimulated thymocytes
Outcome group
Gene expression · Immune and inflammation
Measurements
increase in Tβ4 content
6.3-fold
1 h after stimulation
Tβ4 share of total cellular protein
0.4%
1 h after stimulation
Source sentences
Summary
An early 6.3-fold increase of the peptide occurred after 1 h of stimulation amounting to 0.4% of the total cellular protein.
1 context sentence(design, population, supporting numbers)
Summary · context
The expression of thymosin beta 4, an ubiquitous peptide of high cellular content, was studied in concanavalin A-stimulated rat thymocytes within the first 3 h after activation of the cells.
This result measures the body's own Thymosin beta-4 (human). Nobody was given the peptide. It is not evidence about taking it.
Show in the study text Report a problemFND-0239473001
02LabEndogenous levelIn vitro (cells)Mechanism
Thymosin beta‑4 biosynthesis, measured by [35S]methionine incorporation, increased in concanavalin A-stimulated rat thymocytes in step with the rise in peptide content.
In plain wordsIn rat immune cells, making of the body's own thymosin beta‑4 rose after concanavalin A, along with its level.
Peptide
Thymosin beta-4 (human)
Outcome
IncreaseTβ4 biosynthesis after ConA
Study
ratn: not reported in source
concanavalin A-stimulated thymocytes
Outcome group
Gene expression · Immune and inflammation
Measurements
Tβ4 share of total protein synthesis
1%
1 h after ConA
Source sentences
Summary
This increase coincided with that of thymosin beta 4 biosynthesis measured by [35S]methionine incorporation. The share of thymosin beta 4 synthesis in total protein synthesis 1 h after addition of concanavalin A amounts to 1% but no elevation of the corresponding mRNA was observed.
1 context sentence(design, population, supporting numbers)
Summary · context
The expression of thymosin beta 4, an ubiquitous peptide of high cellular content, was studied in concanavalin A-stimulated rat thymocytes within the first 3 h after activation of the cells.
This result measures the body's own Thymosin beta-4 (human). Nobody was given the peptide. It is not evidence about taking it.
Show in the study text Report a problemFND-0239473002
03LabEndogenous levelIn vitro (cells)Mechanism
No elevation of thymosin beta‑4 mRNA was observed 1 h after concanavalin A, which suggests translational control.
In plain wordsIn rat immune cells, the mRNA for the body's own thymosin beta‑4 did not rise after concanavalin A.
Peptide
Thymosin beta-4 (human)
Outcome
No significant changeTβ4 mRNA after ConA
Study
ratn: not reported in source
concanavalin A-stimulated thymocytes
Outcome group
Gene expression · Immune and inflammation
Source sentences
Summary
but no elevation of the corresponding mRNA was observed.
1 context sentence(design, population, supporting numbers)
Summary · context
The expression of thymosin beta 4, an ubiquitous peptide of high cellular content, was studied in concanavalin A-stimulated rat thymocytes within the first 3 h after activation of the cells.
This result measures the body's own Thymosin beta-4 (human). Nobody was given the peptide. It is not evidence about taking it.
Show in the study text Report a problemFND-0239473003
04LabEndogenous levelIn vitro (cells)Mechanism
Actinomycin D did not inhibit thymosin beta‑4 induction in rat thymocytes, in contrast to cycloheximide.
In plain wordsIn rat immune cells, actinomycin D did not block the rise of the body's own thymosin beta‑4, but cycloheximide did.
Peptide
Thymosin beta-4 (human)
Outcome
MixedEffect of actinomycin D and cycloheximide on Tβ4 induction
Study
ratn: not reported in source
concanavalin A-stimulated thymocytes
Outcome group
Gene expression · Immune and inflammation
Source sentences
Summary
Consequently, actinomycin D did not inhibit thymosin beta 4 induction in contrast to cycloheximide.
1 context sentence(design, population, supporting numbers)
Summary · context
The expression of thymosin beta 4, an ubiquitous peptide of high cellular content, was studied in concanavalin A-stimulated rat thymocytes within the first 3 h after activation of the cells.
This result measures the body's own Thymosin beta-4 (human). Nobody was given the peptide. It is not evidence about taking it.
Show in the study text Report a problemFND-0239473004
05LabEndogenous levelIn vitro (cells)Mechanism
The peaks of maximal thymosin beta‑4 levels and biosynthesis were followed by rapid decreases of both parameters.
In plain wordsIn rat immune cells, the body's own thymosin beta‑4 level and making fell quickly after the peak.
Peptide
Thymosin beta-4 (human)
Outcome
DecreaseDecline of Tβ4 level and biosynthesis after peak
Study
ratn: not reported in source
concanavalin A-stimulated thymocytes
Outcome group
Gene expression · Immune and inflammation
Source sentences
Summary
The peaks of maximal thymosin beta 4 levels and biosynthesis were followed by rapid decreases of these parameters
1 context sentence(design, population, supporting numbers)
Summary · context
The expression of thymosin beta 4, an ubiquitous peptide of high cellular content, was studied in concanavalin A-stimulated rat thymocytes within the first 3 h after activation of the cells.
This result measures the body's own Thymosin beta-4 (human). Nobody was given the peptide. It is not evidence about taking it.
Show in the study text Report a problemFND-0239473005

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
13 Oct 2026, 01:52 UTC
Study results updated
Version 1
12 Oct 2026, 18:17 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

Publication types

Journal Article

MeSH terms

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