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Study · Ex vivo tissue · rat · 1990

3 results

Melanotropin receptors in the brain are differentially distributed and recognize both corticotropin and alpha-melanocyte stimulating hormone.

Tatro JB

Brain research1 Dec 1990Cited by 91 (Europe PMC)

PMID1964830 (opens in a new tab)DOI10.1016/0006-8993(90)90016-5 (opens in a new tab)PepBankPUB-01964830
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study3

3 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabEx vivo tissueActivity measurement

    In rat brain sections, specific [125I]NDP-MSH binding sites followed a region-specific pattern across the septal area, hypothalamus, thalamus, epithalamus, olfactostriatal complex and midbrain.

    In plain wordsIn lab tests on rat brain sections, NDP-MSH bound to specific sites in many brain regions, each with its own pattern.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    Not applicableDistribution of NDP-MSH binding sites in brain
    Study

    ratn: not reported in source

    frozen rat brain sections
    Outcome group
    Receptor activity and mechanism · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Specific (alpha-MSH-inhibitable) [125I]NDP-MSH binding sites were distributed in a region-specific pattern, and were present in numerous structures within the septal area, hypothalamus, thalamus, epithalamus, olfactostriatal complex, and midbrain.
    2 context sentences
    Summary · context
    Each brain structure studied showed a characteristic, reproducible distribution and relative intensity of binding.
    Summary · context
    To determine the distribution of brain melanotropin receptors, we used biologically active [125I]Nle4, D-Phe7-alpha-MSH ([125I]NDP-MSH) for in situ binding and autoradiography in frozen rat brain sections.
  2. 02LabEx vivo tissueActivity measurement

    In rat brain sections, alpha-MSH, NDP-MSH and ACTH gave comparable maximal inhibition of tracer binding in all brain structures, so all detectable sites recognized all three.

    In plain wordsIn lab tests on rat brain sections, NDP-MSH, alpha-MSH and ACTH all blocked tracer binding to a similar maximum in every brain structure.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    Not applicableMaximal inhibition of tracer binding by alpha-MSH, NDP-MSH and ACTH
    Study

    ratn: not reported in source

    frozen rat brain sections
    Outcome group
    Receptor activity and mechanism · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Receptor peptide selectivity was assessed by comparing the dose-response relationships for inhibition of binding by alpha-MSH, NDP-MSH and ACTH.
    2 context sentences
    Summary · context
    In all brain structures studied, the 3 peptides gave comparable maximal inhibition of tracer binding, indicating that all detectable binding sites recognized all 3 melanotropins.
    Summary · context
    To determine the distribution of brain melanotropin receptors, we used biologically active [125I]Nle4, D-Phe7-alpha-MSH ([125I]NDP-MSH) for in situ binding and autoradiography in frozen rat brain sections.
  3. 03LabEx vivo tissueActivity measurement

    In rat brain sections, NDP-MSH (EC50 1.7 nM) was more potent than alpha-MSH (EC50 46.9 nM), and alpha-MSH was as potent as ACTH.

    In plain wordsIn lab tests on rat brain sections, NDP-MSH was much more potent than alpha-MSH and ACTH in inhibiting tracer binding.

    Peptide
    Melanotan I (afamelanotide)
    Outcome
    Not applicableRelative potency of NDP-MSH (EC50)
    Study

    ratn: not reported in source

    frozen rat brain sections
    Outcome group
    Receptor activity and mechanism · Nervous system and cognition
    Studied dose
    Dose not stated

    Measurements

    EC50
    1.7 nM
    rat brain sections, NDP-MSH

    Source sentences

    Summary
    The respective relative potencies were: NDP-MSH (EC50 = 1.7 +/- 0.6 nM) greater than alpha-MSH (EC50 = 46.9 +/- 11.7 nM) = ACTH.
    1 context sentence
    Summary · context
    To determine the distribution of brain melanotropin receptors, we used biologically active [125I]Nle4, D-Phe7-alpha-MSH ([125I]NDP-MSH) for in situ binding and autoradiography in frozen rat brain sections.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 21:44 UTC

    Study results updated

  2. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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