Rat thymosin beta 4 gene. Intron-containing gene and multiple retroposons.

Varghese S, Kronenberg HM

The Journal of biological chemistry1 Aug 1991Cited by 10 (Europe PMC)

PMID1650352DOI10.1016/s0021-9258(18)98676-xPepBankPUB-01650352

Publisher page PubMedAsk about this study

Summary

Thymosin beta 4 (T beta 4), a 43-residue peptide of uncertain function, is widely distributed in most tissues of vertebrates. Southern blot analysis demonstrated that the rat genome contains many closely related T beta 4 sequences. Two distinct T beta 4 sequences were isolated by screening a rat genomic DNA library. Analysis of these clones revealed that they represent T beta 4 retroposons; they lack introns and contain stretches of poly(A) sequence at their 3' ends and direct repeats flanking the cDNA-like sequences. In order to isolate a T beta 4 intron-containing gene, DNA fragments that include T beta 4 introns were generated by polymerase chain reaction, using rat genomic DNA as template. Subsequent Southern blot analysis of rat genomic DNA with intron-specific probes demonstrated the presence of a single T beta 4 intron-containing gene. Upon screening the rat genomic DNA library with an intron-specific probe, two overlapping clones of the T beta 4 intron-containing gene were isolated. Further characterization and sequence analysis showed that the rat T beta 4 intron-containing gene has a 2-kilobase pair-long transcription unit containing two introns. The transcription initiation site and putative regulatory elements of the promoter were identified.

CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study4

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone4

In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Thymosin beta-4 (human).

01LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cell-free)Other
Southern blot analysis showed that the rat genome contains many closely related thymosin beta‑4 sequences.
In plain wordsIn lab tests on rat DNA, many closely related copies of the thymosin beta‑4 sequence were found.
Peptide
Thymosin beta-4 (human)
Outcome
Not applicableNumber of thymosin beta 4 sequences in the rat genome
Study
ratn: not reported in source
Rat genomic DNA
Outcome group
Peptide structure and chemistry · Whole body
Source sentences
Summary
Southern blot analysis demonstrated that the rat genome contains many closely related T beta 4 sequences.
In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).
Show in the study text Report a problemFND-0165035201
02LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cell-free)Other
Two thymosin beta‑4 sequences isolated from a rat genomic library were retroposons that lack introns and have poly(A) stretches and flanking direct repeats.
In plain wordsIn lab tests on rat DNA, two thymosin beta‑4 copies were gene fragments without introns.
Peptide
Thymosin beta-4 (human)
Outcome
Not applicableStructure of two isolated thymosin beta 4 sequences
Study
ratn: not reported in source
Rat genomic DNA
Outcome group
Peptide structure and chemistry · Whole body
Source sentences
Summary
Two distinct T beta 4 sequences were isolated by screening a rat genomic DNA library. Analysis of these clones revealed that they represent T beta 4 retroposons; they lack introns and contain stretches of poly(A) sequence at their 3' ends and direct repeats flanking the cDNA-like sequences.
In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).
Show in the study text Report a problemFND-0165035202
03LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cell-free)Other
Southern blot analysis with intron-specific probes showed a single intron-containing thymosin beta‑4 gene in rat genomic DNA.
In plain wordsIn lab tests on rat DNA, only one thymosin beta‑4 copy was a full gene with introns.
Peptide
Thymosin beta-4 (human)
Outcome
Not applicableIntron-containing thymosin beta 4 gene
Study
ratn: not reported in source
Rat genomic DNA
Outcome group
Peptide structure and chemistry · Whole body
Source sentences
Summary
Subsequent Southern blot analysis of rat genomic DNA with intron-specific probes demonstrated the presence of a single T beta 4 intron-containing gene.
In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).
Show in the study text Report a problemFND-0165035203
04LabNot a test of Thymosin beta-4 (human) aloneIn vitro (cell-free)Other
The rat thymosin beta‑4 intron-containing gene has a 2-kilobase pair transcription unit with two introns.
In plain wordsIn lab tests on rat DNA, the thymosin beta‑4 gene was found to have two introns.
Peptide
Thymosin beta-4 (human)
Outcome
Not applicableTranscription unit of the rat thymosin beta 4 gene
Study
ratn: not reported in source
Rat genomic DNA
Outcome group
Peptide structure and chemistry · Whole body
Measurements
transcription unit length
2 kilobase pair
rat T beta 4 intron-containing gene
Source sentences
Summary
Further characterization and sequence analysis showed that the rat T beta 4 intron-containing gene has a 2-kilobase pair-long transcription unit containing two introns.
In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).
Show in the study text Report a problemFND-0165035204

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Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
13 Oct 2026, 01:52 UTC
Study results updated
Version 1
12 Oct 2026, 18:17 UTC
First added · source update from Europe PMC

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