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Study · In vitro (cell-free) · rabbit · 1992

5 results

A hypothalamic activator of calmodulin-dependent enzymes is thymosin beta 4 (1-39).

Galoyan AA, Gurvitis BYa, Shuvalova LA, Davis MT, Shively JE, Lee TD

Neurochemical research1 Aug 1992Cited by 13 (Europe PMC)

PMID1641059 (opens in a new tab)DOI10.1007/bf00969011 (opens in a new tab)PepBankPUB-01641059
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study5

5 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cell-free)Activity measurement

    In a cell-free assay, thymosin alpha‑1 was a Ca(2+)-independent high-affinity stimulator of myosin light chain kinase basal activity.

    In plain wordsIn lab tests with an isolated enzyme, thymosin alpha‑1 increased the activity of myosin light chain kinase.

    Peptide
    Thymosin alpha-1
    Outcome
    IncreaseStimulation of MLCK basal activity
    Study

    n: not reported in source

    Rabbit skeletal muscle MLCK
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Evidence is presented that all the indicated compounds are Ca(2+)-independent high-affinity MLCK stimulators.
    1 context sentence
    Summary · context
    The stimulating effect of C3 on rabbit skeletal muscle MLCK basal activity was compared with that of thymosin alpha 1 and thymosin beta 4 (16-38).
  2. 02LabIn vitro (cell-free)Activity measurement

    In a cell-free assay, thymosin alpha‑1 was the least potent MLCK activator among C3, thymosin beta 4 and calmodulin with Ca2+.

    In plain wordsIn lab tests with an isolated enzyme, thymosin alpha‑1 activated the enzyme less strongly than the other compounds tested.

    Peptide
    Thymosin alpha-1
    Outcome
    Not applicableRelative potency for MLCK activation
    Study

    n: not reported in source

    Rabbit skeletal muscle MLCK
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    The potency of the stimulators in activating the enzyme was: C3 greater than beta 4 greater than (CaM+Ca2+) greater than alpha 1.
    1 context sentence
    Summary · context
    The stimulating effect of C3 on rabbit skeletal muscle MLCK basal activity was compared with that of thymosin alpha 1 and thymosin beta 4 (16-38).
  3. 03LabIn vitro (cell-free)Activity measurement

    In a cell-free assay with rabbit skeletal muscle myosin light chain kinase, thymosin beta‑4 (16-38) stimulated basal enzyme activity independent of Ca2+.

    In plain wordsIn lab tests, a piece of thymosin beta‑4 raised the basal activity of a muscle enzyme, and this did not need calcium.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    IncreaseStimulation of MLCK basal activity
    Study

    rabbitn: not reported in source

    purified rabbit skeletal muscle myosin light chain kinase (MLCK)
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    The stimulating effect of C3 on rabbit skeletal muscle MLCK basal activity was compared with that of thymosin alpha 1 and thymosin beta 4 (16-38). Evidence is presented that all the indicated compounds are Ca(2+)-independent high-affinity MLCK stimulators.
  4. 04LabIn vitro (cell-free)Activity measurement

    In a cell-free assay, thymosin beta‑4 (16-38) was a more potent MLCK stimulator than calmodulin with Ca2+ and thymosin alpha 1, and less potent than C3.

    In plain wordsIn lab tests, a piece of thymosin beta‑4 activated the muscle enzyme more than thymosin alpha 1 and less than C3.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    Not applicablePotency for activating MLCK versus other stimulators
    Study

    rabbitn: not reported in source

    purified rabbit skeletal muscle myosin light chain kinase (MLCK)
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    The potency of the stimulators in activating the enzyme was: C3 greater than beta 4 greater than (CaM+Ca2+) greater than alpha 1.
  5. 05LabIn vitro (cell-free)Activity measurement

    In a cell-free assay with rabbit skeletal muscle myosin light chain kinase, C3, tentatively identified as thymosin beta‑4 (1-39), stimulated basal enzyme activity independent of Ca2+.

    In plain wordsIn lab tests, C3, tentatively a form of thymosin beta‑4, raised the basal activity of a muscle enzyme without needing calcium.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    IncreaseStimulation of MLCK basal activity by C3
    Study

    rabbitn: not reported in source

    purified rabbit skeletal muscle myosin light chain kinase (MLCK)
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    The stimulating effect of C3 on rabbit skeletal muscle MLCK basal activity was compared with that of thymosin alpha 1 and thymosin beta 4 (16-38). Evidence is presented that all the indicated compounds are Ca(2+)-independent high-affinity MLCK stimulators.
    1 context sentence
    Summary · context
    One of these stimulators, denoted as C3, has been purified to homogeneity by reverse phase HPLC and tentatively identified as thymosin beta 4 (1-39) by mass spectrometry and Edman microsequence analysis.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 4Current

    12 Oct 2026, 18:17 UTC

    Study data updated · source update from Europe PMC

  2. Version 3

    10 Oct 2026, 08:55 UTC

    Study results updated

  3. Version 2

    9 Oct 2026, 03:03 UTC

    Study data updated · source update from Europe PMC

  4. Version 1

    9 Oct 2026, 03:02 UTC

    First added · source update from Europe PMC

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