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Study · Measured the body's own peptide · 1992

3 results

Detection of thymosin beta 4 in situ in a guinea pig cerebral cortex preparation using 1H NMR spectroscopy.

Kauppinen RA, Nissinen T, Kärkkäinen AM, Pirttilä TR, Palvimo J, Kokko H, Williams SR

The Journal of biological chemistry1 May 1992Cited by 30 (Europe PMC)

PMID1577821 (opens in a new tab)DOI10.1016/s0021-9258(19)50178-8 (opens in a new tab)PepBankPUB-01577821
Publisher pagePubMedAsk about this study
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Summary

In the present work we have investigated the macromolecules that contribute to the brain 1H NMR spectrum. The cerebral cortex showed distinct resonances at the uncrowded methyl- and methylene chemical shift scale of the spin-echo 1H NMR spectrum. The peaks at 1.22 and 1.40 ppm (relative to the methyl protons of N-acetyl aspartate at 2.02 ppm) arise from cerebral macromolecules without evidence for co-resonances from low molecular weight metabolites as shown by the spin-spin relaxation decays of these resonances. In addition to these NMR signals, peaks at 0.9 and 1.7 ppm from macromolecules were detected. These resonances are from proteins, and we have identified the polypeptides that contributed to the 1H NMR peaks. Two proteins that were present at concentrations of 250 and 350 micrograms/g of dryed tissue showed 1H NMR spectra that resembled the macromolecular pattern in the cerebral 1H NMR spectrum. They were identified as thymosin beta 4 and histone H1, respectively. Thymosin beta 4 was present in soluble high speed cytoplasmic fraction and in P2 pellet, whereas histone H1 was detected in nuclear enriched fraction. A chemical shift-correlated two-dimensional 1H NMR spectrum of thymosin beta 4 in vitro revealed a coupling pattern that matched the macromolecule in the cerebral cortex which we have previously noted (Kauppinen R. A., Kokko, H., and Williams, S. R. (1992) J. Neurochem. 58, 967-974). On the basis of both one- and two-dimensional NMR evidence, subcellular distribution and high concentration, we assign the 1H NMR signals at 0.9, 1.22, 1.40, and 1.7 ppm in the cerebral cortex to thymosin beta 4.
CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study3

No result from this study comes from a test of the peptide.

Natural levels measured2

These results measure the body's own peptide. Nobody was given the peptide. They are not evidence about taking it. They are left out of key results, evidence counts and charts.

  1. 01AnimalEndogenous levelAnimal study (in vivo)Other

    In guinea pig cerebral cortex, thymosin beta‑4 was present at a concentration of 250 micrograms per gram of dried tissue.

    In plain wordsIn guinea pig brain cortex, the body's own thymosin beta‑4 was found at 250 micrograms per gram of dried tissue.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    Not applicableThymosin beta 4 concentration in cerebral cortex
    Study

    guinea pign: not reported in source

    Cerebral cortex preparation, 1H NMR spectroscopy
    Outcome group
    Endogenous peptide levels · Nervous system and cognition

    Measurements

    concentration
    250 µg/g dried tissue
    guinea pig cerebral cortex

    Source sentences

    Summary
    Two proteins that were present at concentrations of 250 and 350 micrograms/g of dryed tissue showed 1H NMR spectra that resembled the macromolecular pattern in the cerebral 1H NMR spectrum. They were identified as thymosin beta 4 and histone H1, respectively.
    1 context sentence
    Summary · context
    In the present work we have investigated the macromolecules that contribute to the brain 1H NMR spectrum.

    This result measures the body's own Thymosin beta-4 (human). Nobody was given the peptide. It is not evidence about taking it.

  2. 02AnimalEndogenous levelAnimal study (in vivo)Other

    In guinea pig cerebral cortex, thymosin beta‑4 was present in the soluble high speed cytoplasmic fraction and in the P2 pellet.

    In plain wordsIn guinea pig brain cortex, the body's own thymosin beta‑4 was found in the soluble cytoplasm fraction and in the P2 pellet.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    Not applicableSubcellular distribution of thymosin beta 4 in cerebral cortex
    Study

    guinea pign: not reported in source

    Cerebral cortex preparation, 1H NMR spectroscopy
    Outcome group
    Endogenous peptide levels · Nervous system and cognition

    Source sentences

    Summary
    Thymosin beta 4 was present in soluble high speed cytoplasmic fraction and in P2 pellet, whereas histone H1 was detected in nuclear enriched fraction.
    1 context sentence
    Summary · context
    In the present work we have investigated the macromolecules that contribute to the brain 1H NMR spectrum.

    This result measures the body's own Thymosin beta-4 (human). Nobody was given the peptide. It is not evidence about taking it.

Studies that did not test the peptide alone1

In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Thymosin beta-4 (human).

  1. 03AnimalNot a test of Thymosin beta-4 (human) aloneAnimal study (in vivo)Other

    On NMR evidence, subcellular distribution and high concentration, the cerebral cortex 1H NMR signals at 0.9, 1.22, 1.40 and 1.7 ppm were assigned to thymosin beta‑4.

    In plain wordsIn guinea pig brain cortex, NMR signals were assigned to thymosin beta‑4. This is a detection result, not an effect of the peptide.

    Peptide
    Thymosin beta-4 (human)
    Outcome
    Not applicableAssignment of cortex 1H NMR signals to thymosin beta 4
    Study

    guinea pign: not reported in source

    Cerebral cortex preparation, 1H NMR spectroscopy
    Outcome group
    Receptor activity and mechanism · Nervous system and cognition

    Measurements

    chemical shift
    0.9 ppm
    cerebral cortex
    chemical shift
    1.22 ppm
    cerebral cortex
    chemical shift
    1.4 ppm
    cerebral cortex
    chemical shift
    1.7 ppm
    cerebral cortex

    Source sentences

    Summary
    On the basis of both one- and two-dimensional NMR evidence, subcellular distribution and high concentration, we assign the 1H NMR signals at 0.9, 1.22, 1.40, and 1.7 ppm in the cerebral cortex to thymosin beta 4.
    1 context sentence
    Summary · context
    In the present work we have investigated the macromolecules that contribute to the brain 1H NMR spectrum.

    In this study, Thymosin beta-4 (human) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin beta-4 (human).

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 01:52 UTC

    Study results updated

  2. Version 1

    12 Oct 2026, 18:17 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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