Stable enhancement of calcium retention in mitochondria isolated from rat liver after the administration of glucagon to the intact animal.

Prpić V, Spencer TL, Bygrave FL

The Biochemical journal1 Dec 1978Cited by 74 (Europe PMC)

PMID747647PMCIDPMC1186292DOI10.1042/bj1760705PepBankPUB-00747647

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study8

8 results from studies that gave the peptide. No result from this study comes from people.

01AnimalAnimal study (in vivo)Mechanism
In rats, glucagon increased hepatic mitochondrial calcium retention to over 45 minutes, versus 6-8 minutes after saline.
In plain wordsIn rats, liver mitochondria retained calcium longer after glucagon than after saline.
Peptide
Glucagon
Outcome
IncreaseCalcium retention
Study
ratn: not reported in source
Rat liver mitochondria after in vivo dosing
Comparator
Saline-treated rats
Outcome group
Mitochondrial function · Liver
Studied dose
30mug/100g body wt.As given in this study (rat).
Measurements
Calcium retention
> 45 min
glucagon
Calcium retention
6 to 8 min
saline
Source sentences
Summary
Mitochondria isolated from rat liver by centrifugation of the homogenate in buffered iso-osmotic sucrose at between 4000 and 8000g-min, 1h after the administration in vivo of 30mug of glucagon/100g body wt., retain Ca(2+) for over 45min after its addition at 100nmol/mg of mitochondrial protein in th…
1 context sentence(design, population, supporting numbers)
Summary · context
In similar experiments, but after the administration of saline (0.9% NaCl) in place of glucagon, Ca(2+) is retained for 6-8min.
Report a problemFND-0074764701
02AnimalAnimal study (in vivo)Mechanism
In rats, hepatic mitochondrial resting respiration after calcium accumulation remained low after glucagon, unlike after saline.
In plain wordsIn rats, liver mitochondrial resting respiration remained lower after glucagon than after saline following calcium accumulation.
Peptide
Glucagon
Outcome
DecreaseResting respiration after calcium accumulation
Study
ratn: not reported in source
Rat liver mitochondria after in vivo dosing
Comparator
Saline-treated rats
Outcome group
Mitochondrial function · Liver
Studied dose
30mug/100g body wt.As given in this study (rat).
Source sentences
Summary
The resting rate of respiration after Ca(2+) accumulation by mitochondria from glucagon-treated rats remains low by contrast with that from saline-treated rats.
Report a problemFND-0074764702
03AnimalAnimal study (in vivo)Mechanism
In rats, glucagon increased the ability of hepatic mitochondria to retain endogenous adenine nucleotides.
In plain wordsIn rats, liver mitochondria retained more adenine nucleotides after glucagon than after saline.
Peptide
Glucagon
Outcome
IncreaseAdenine nucleotide retention
Study
ratn: not reported in source
Rat liver mitochondria after in vivo dosing
Comparator
Saline-treated rats
Outcome group
Mitochondrial function · Liver
Studied dose
30mug/100g body wt.As given in this study (rat).
Source sentences
Summary
Concomitant with the enhanced retention of Ca(2+) and low rates of resting respiration by mitochondria from glucagon-treated rats was an increased ability to retain endogenous adenine nucleotides.
Report a problemFND-0074764703
04AnimalAnimal study (in vivo)Mechanism
In rats, hepatic mitochondrial adenine nucleotide concentrations were significantly higher after glucagon treatment.
In plain wordsIn rats, liver mitochondrial adenine nucleotide concentrations were higher after glucagon than after saline.
Peptide
Glucagon
Outcome
IncreaseAdenine nucleotide concentration
Study
ratn: not reported in source
Rat liver mitochondria after in vivo dosing
Comparator
Saline-treated rats
Outcome group
Mitochondrial function · Liver
Studied dose
30mug/100g body wt.As given in this study (rat).
Source sentences
Summary
An investigation of properties of mitochondria known to influence Ca(2+) transport revealed a significantly higher concentration of adenine nucleotides but not of P(i) in those from glucagon-treated rats.
Report a problemFND-0074764704
05AnimalAnimal study (in vivo)Mechanism
In rats, glucagon did not significantly increase hepatic mitochondrial phosphate concentration.
In plain wordsIn rats, liver mitochondrial phosphate concentrations did not significantly increase after glucagon compared with saline.
Peptide
Glucagon
Outcome
No significant changeMitochondrial phosphate concentration
Study
ratn: not reported in source
Rat liver mitochondria after in vivo dosing
Comparator
Saline-treated rats
Outcome group
Mitochondrial function · Liver
Studied dose
30mug/100g body wt.As given in this study (rat).
Source sentences
Summary
An investigation of properties of mitochondria known to influence Ca(2+) transport revealed a significantly higher concentration of adenine nucleotides but not of P(i) in those from glucagon-treated rats.
Report a problemFND-0074764705
06AnimalAnimal study (in vivo)Mechanism
In glucagon-treated rats, hepatic mitochondrial membrane potential remained unchanged.
In plain wordsIn rats, liver mitochondrial membrane potential remained unchanged after glucagon compared with saline.
Peptide
Glucagon
Outcome
No significant changeMitochondrial membrane potential
Study
ratn: not reported in source
Rat liver mitochondria after in vivo dosing
Comparator
Saline-treated rats
Outcome group
Mitochondrial function · Liver
Studied dose
30mug/100g body wt.As given in this study (rat).
Source sentences
Summary
The membrane potential remained unchanged, but the transmembrane pH gradient increased by approx. 10mV, indicating increased alkalinity of the matrix space.
Report a problemFND-0074764706
07AnimalAnimal study (in vivo)Mechanism
In glucagon-treated rats, the hepatic mitochondrial transmembrane pH gradient increased by approximately 10mV.
In plain wordsIn rats, the liver mitochondrial pH gradient increased by approximately 10mV after glucagon compared with saline.
Peptide
Glucagon
Outcome
IncreaseTransmembrane pH gradient
Study
ratn: not reported in source
Rat liver mitochondria after in vivo dosing
Comparator
Saline-treated rats
Outcome group
Mitochondrial function · Liver
Studied dose
30mug/100g body wt.As given in this study (rat).
Measurements
Transmembrane pH gradient increase
≈ 10 mV
glucagon
Source sentences
Summary
The membrane potential remained unchanged, but the transmembrane pH gradient increased by approx. 10mV, indicating increased alkalinity of the matrix space.
Report a problemFND-0074764707
08AnimalAnimal study (in vivo)Mechanism
In rats, glucagon treatment did not affect hepatic mitochondrial adenine nucleotide translocase activity.
In plain wordsIn rats, liver mitochondrial adenine nucleotide translocase activity did not change after glucagon compared with saline.
Peptide
Glucagon
Outcome
No significant changeAdenine nucleotide translocase activity
Study
ratn: not reported in source
Rat liver mitochondria after in vivo dosing
Comparator
Saline-treated rats
Outcome group
Mitochondrial function · Liver
Studied dose
30mug/100g body wt.As given in this study (rat).
Source sentences
Summary
The activity of the adenine nucleotide translocase was unaffected by glucagon treatment of rats in vivo.
Report a problemFND-0074764708

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
28 Oct 2026, 02:03 UTC
Study results updated
Version 1
27 Oct 2026, 16:35 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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