Pyridine nucleotide cycle of Salmonella typhimurium: isolation and characterization of pncA, pncB, and pncC mutants and utilization of exogenous nicotinamide adenine dinucleotide.

Foster JW, Kinney DM, Moat AG

Journal of bacteriology1 Mar 1979Cited by 44 (Europe PMC)

PMID220211PMCIDPMC218297DOI10.1128/jb.137.3.1165-1175.1979PepBankPUB-00220211

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study5

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone5

In this study, NAD+ (nicotinamide adenine dinucleotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking NAD+ (nicotinamide adenine dinucleotide).

01LabNot a test of NAD+ (nicotinamide adenine dinucleotide) aloneIn vitro (cells)Mechanism
In Salmonella typhimurium mutants, growth experiments provided evidence for an alternate NAD recycling pathway.
In plain wordsIn cells, growth experiments in Salmonella mutants supported an alternate NAD recycling pathway.
Peptide
NAD+ (nicotinamide adenine dinucleotide)
Outcome
Not applicableAlternate NAD recycling pathway
Study
Salmonella typhimuriumn: not reported in source
Salmonella typhimurium LT-2 mutants
Outcome group
Receptor activity and mechanism · Pharmacology
Source sentences
Summary
Growth experiments using double mutants blocked in the de novo pathway to nicotinamide adenine dinucleotide (NAD) (nad) and in the pyridine nucleotide cycle (pnc) at either the pncA or pncB locus, or both,
2 context sentences(design, population, supporting numbers)
Summary · context
have provided evidence for the existence of an alternate recycling pathway in this organism.
Summary · context
Utilization of exogenous NAD was examined through the use of [14C]carbonyl-labeled NAD and [14C]adenine-labeled NAD.
In this study, NAD+ (nicotinamide adenine dinucleotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking NAD+ (nicotinamide adenine dinucleotide).
Report a problemFND-0022021101
02LabNot a test of NAD+ (nicotinamide adenine dinucleotide) aloneIn vitro (cells)Mechanism
In Salmonella typhimurium, labeled-NAD experiments suggested that NAD was degraded to nicotinamide mononucleotide at the cell surface.
In plain wordsIn cells, Salmonella experiments suggested that NAD was degraded at the cell surface.
Peptide
NAD+ (nicotinamide adenine dinucleotide)
Outcome
Not applicableCell-surface NAD degradation
Study
Salmonella typhimuriumn: not reported in source
Salmonella typhimurium LT-2 mutants
Outcome group
Receptor activity and mechanism · Pharmacology
Source sentences
Summary
The results of these experiments suggest that NAD is degraded to nicotinamide mononucleotide at the cell surface.
1 context sentence(design, population, supporting numbers)
Summary · context
Utilization of exogenous NAD was examined through the use of [14C]carbonyl-labeled NAD and [14C]adenine-labeled NAD.
In this study, NAD+ (nicotinamide adenine dinucleotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking NAD+ (nicotinamide adenine dinucleotide).
Report a problemFND-0022021102
03LabNot a test of NAD+ (nicotinamide adenine dinucleotide) aloneIn vitro (cells)Mechanism
In Salmonella typhimurium, some extracellular NAD-derived nicotinamide mononucleotide entered cells through glycohydrolase-mediated transport and degradation to nicotinamide.
In plain wordsIn cells, some NAD-derived material entered Salmonella cells and was degraded to nicotinamide.
Peptide
NAD+ (nicotinamide adenine dinucleotide)
Outcome
Not applicableTransport and degradation of NAD-derived mononucleotide
Study
Salmonella typhimuriumn: not reported in source
Salmonella typhimurium LT-2 mutants
Outcome group
Receptor activity and mechanism · Pharmacology
Source sentences
Summary
A portion of this extracellular nicotinamide mononucleotide is then transported across the cell membrane by nicotinamide mononucleotide glycohydrolase and degraded to nicotinamide in the process.
1 context sentence(design, population, supporting numbers)
Summary · context
Utilization of exogenous NAD was examined through the use of [14C]carbonyl-labeled NAD and [14C]adenine-labeled NAD.
In this study, NAD+ (nicotinamide adenine dinucleotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking NAD+ (nicotinamide adenine dinucleotide).
Report a problemFND-0022021103
04LabNot a test of NAD+ (nicotinamide adenine dinucleotide) aloneIn vitro (cells)Mechanism
In Salmonella typhimurium, the remaining NAD-derived nicotinamide mononucleotide accumulated extracellularly.
In plain wordsIn cells, some NAD-derived material accumulated outside Salmonella cells.
Peptide
NAD+ (nicotinamide adenine dinucleotide)
Outcome
IncreaseExtracellular accumulation of NAD-derived mononucleotide
Study
Salmonella typhimuriumn: not reported in source
Salmonella typhimurium LT-2 mutants
Outcome group
Receptor activity and mechanism · Pharmacology
Source sentences
Summary
The remaining nicotinamide mononucleotide accumulates extracellularly and will support the growth of nadA pncB mutants which cannot utilize the nicotinamide resulting from the major pathway of NAD degradation.
1 context sentence(design, population, supporting numbers)
Summary · context
Utilization of exogenous NAD was examined through the use of [14C]carbonyl-labeled NAD and [14C]adenine-labeled NAD.
In this study, NAD+ (nicotinamide adenine dinucleotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking NAD+ (nicotinamide adenine dinucleotide).
Report a problemFND-0022021104
05LabNot a test of NAD+ (nicotinamide adenine dinucleotide) aloneIn vitro (cells)Activity measurement
In Salmonella typhimurium, extracellular NAD-derived nicotinamide mononucleotide supported growth of nadA pncB mutants unable to use nicotinamide.
In plain wordsIn cells, NAD-derived material outside Salmonella cells supported growth of mutants that could not use nicotinamide.
Peptide
NAD+ (nicotinamide adenine dinucleotide)
Outcome
IncreaseGrowth support in nadA pncB mutants
Study
Salmonella typhimuriumn: not reported in source
Salmonella typhimurium LT-2 mutants
Outcome group
Receptor activity and mechanism · Pharmacology
Source sentences
Summary
The remaining nicotinamide mononucleotide accumulates extracellularly and will support the growth of nadA pncB mutants which cannot utilize the nicotinamide resulting from the major pathway of NAD degradation.
1 context sentence(design, population, supporting numbers)
Summary · context
Utilization of exogenous NAD was examined through the use of [14C]carbonyl-labeled NAD and [14C]adenine-labeled NAD.
In this study, NAD+ (nicotinamide adenine dinucleotide) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking NAD+ (nicotinamide adenine dinucleotide).
Report a problemFND-0022021105

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
14 Oct 2026, 05:02 UTC
Study results updated
Version 1
14 Oct 2026, 01:46 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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MeSH terms

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