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Study · Ex vivo tissue · 2001

4 results

Purification and biological activity of recombinant thymosin alpha 1

Shi Jihong, Zhang Yingqi, Zhao Ning, Yan Zhen, Han Wei, Zhao Yongtong

Journal of the Fourth Military Medical University1 Jan 2001

PepBankPUB-97754522
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Summary

The text of this summary is not shown here, because the article license does not allow reuse.

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study4

2 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabEx vivo tissueActivity measurement

    In spleen lymphocytes, recombinant thymosin alpha‑1 and its fusion protein had biological activity similar to synthesized thymosin alpha‑1.

    In plain wordsIn lab tests on spleen cells, lab-made thymosin alpha‑1 worked about as well as the synthetic form.

    Peptide
    Thymosin alpha-1
    Outcome
    No significant changeBiological activity versus synthesized thymosin alpha 1
    Study

    n: not reported in source

    ConA-stimulated spleen lymphocytes
    Comparator
    Synthesized thymosin alpha 1
    Outcome group
    Immune response · Immune and inflammation
    Studied dose
    Dose not stated

    Source sentences

    Summary
    They results showed that the fusion protein and T#alpha#_(1) had the similar biological activity to that of the synthesized T#alpha#_(1).
    1 context sentence
    Summary · context
    Their biological activity was analysed by ~(3)H-TdR incorparation.
  2. 02LabEx vivo tissueActivity measurement

    In ConA-stimulated spleen lymphocytes, recombinant thymosin alpha‑1 and its fusion protein increased the proliferative response.

    In plain wordsIn lab tests on spleen cells, lab-made thymosin alpha‑1 increased immune cell growth.

    Peptide
    Thymosin alpha-1
    Outcome
    IncreaseProliferation of ConA-stimulated spleen lymphocytes
    Study

    n: not reported in source

    ConA-stimulated spleen lymphocytes
    Outcome group
    Immune response · Immune and inflammation
    Studied dose
    Dose not stated

    Source sentences

    Summary
    They could increase the proliferative response of the mitogen ConA stimulated spleen lymphocytes.

Studies that did not test the peptide alone2

In this study, Thymosin alpha-1 was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Thymosin alpha-1.

  1. 03LabNot a test of Thymosin alpha-1 aloneOther designOther

    In a bacterial expression system, the induced fusion protein band made up 400 g/kg of total bacterial proteins.

    In plain wordsIn lab-grown bacteria, a thymosin alpha‑1 fusion protein made up 400 g per kg of the bacterial protein.

    Peptide
    Thymosin alpha-1
    Outcome
    Not applicableFusion protein share of bacterial protein
    Study

    n: not reported in source

    Bacterial expression system
    Outcome group
    Synthesis and manufacturing · Pharmacology

    Measurements

    Fusion protein share
    400 g·kg~(-1)
    total bacterial proteins

    Source sentences

    Summary
    SDS-PAGE analysis showed an induced expression fusion protein band which consititued 400 g·kg~(-1) of the total bacterial proteins.

    In this study, Thymosin alpha-1 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin alpha-1.

  2. 04LabNot a test of Thymosin alpha-1 aloneOther designOther

    After CNBr cleavage and ion exchange purification, HPLC showed a thymosin alpha‑1 purity of 950 g/kg.

    In plain wordsIn the lab, purified recombinant thymosin alpha‑1 had a purity of 950 g per kg.

    Peptide
    Thymosin alpha-1
    Outcome
    Not applicablePurity of recombinant thymosin alpha 1 after cleavage
    Study

    n: not reported in source

    Bacterial expression system
    Outcome group
    Synthesis and manufacturing · Pharmacology

    Measurements

    Purity
    950 g·kg~(-1)
    HPLC

    Source sentences

    Summary
    HPLC showed the purity of T#alpha#_(1) was 950 g·kg~(-1).
    1 context sentence
    Summary · context
    After CNBr cleavage of the fusion protein, T#alpha#_(1) was obtained by ion exchange methods and proved by 2L-Tricine-SDS-PAGE analysis.

    In this study, Thymosin alpha-1 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin alpha-1.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 3Current

    12 Oct 2026, 17:28 UTC

    Study results updated

  2. Version 2

    9 Oct 2026, 03:03 UTC

    Study data updated · source update from Europe PMC

  3. Version 1

    9 Oct 2026, 03:02 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

The source lists no publication types, MeSH terms or keywords.

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