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Study · In vitro (cells) · 2012

4 results

Expression and purification of two alternative peptides for mechano-growth factor in Escherichia coli

Song L, Zhu WS, Dong JL, Li ZY, Liu XH

Biotechnology letters1 Feb 2012

PepBankPUB-93793503
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Summary

The text of this summary is not shown here, because the article license does not allow reuse.

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study4

2 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Efficacy outcome

    Recombinant MGFc40 and MGFc24 stimulated proliferation of C2C12 cells.

    In plain wordsIn cells, both MGF E peptides made muscle cells multiply more.

    Peptide
    MGF / PEG-MGF
    Outcome
    IncreaseC2C12 cell proliferation
    Study

    n: not reported in source

    C2C12 cells
    Outcome group
    Muscle injury and repair · Musculoskeletal
    Studied dose
    Dose not stated

    Source sentences

    Summary
    stimulated cell proliferation and inhibited cell differentiation of C2C12 cells.
  2. 02LabIn vitro (cells)Efficacy outcome

    Recombinant MGFc40 and MGFc24 inhibited differentiation of C2C12 cells.

    In plain wordsIn cells, both MGF E peptides slowed the maturing of muscle cells.

    Peptide
    MGF / PEG-MGF
    Outcome
    DecreaseC2C12 cell differentiation
    Study

    n: not reported in source

    C2C12 cells
    Outcome group
    Muscle injury and repair · Musculoskeletal
    Studied dose
    Dose not stated

    Source sentences

    Summary
    inhibited cell differentiation of C2C12 cells.

Studies that did not test the peptide alone2

In this study, MGF / PEG-MGF was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking MGF / PEG-MGF.

  1. 03LabNot a test of MGF / PEG-MGF aloneOther designOther

    Recombinant MGFc40 protein was expressed in E. coli and purified by affinity chromatography at 4 mg per litre.

    In plain wordsIn lab tests, bacteria made the MGFc40 peptide at 4 mg per litre.

    Peptide
    MGF / PEG-MGF
    Outcome
    Not applicableYield of recombinant MGFc40 protein
    Study

    n: not reported in source

    Outcome group
    Synthesis and manufacturing · Pharmacology

    Measurements

    Yield
    4 mg l−1
    MGFc40

    Source sentences

    Summary
    Recombinant MGFc40 protein (4 mg l−1) was expressed and purified by affinity chromatography using His60 Ni Superflow.

    In this study, MGF / PEG-MGF was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking MGF / PEG-MGF.

  2. 04LabNot a test of MGF / PEG-MGF aloneOther designOther

    After cleavage of the GST tail, 1 mg of MGFc24 protein per litre was obtained.

    In plain wordsIn lab tests, bacteria made the MGFc24 peptide at 1 mg per litre after purification.

    Peptide
    MGF / PEG-MGF
    Outcome
    Not applicableYield of recombinant MGFc24 protein
    Study

    n: not reported in source

    Outcome group
    Synthesis and manufacturing · Pharmacology

    Measurements

    Yield
    1 mg l−1
    MGFc24

    Source sentences

    Summary
    After enzymatic cleavage of the GST-tail, 1 mg MGFc24 protein l−1 was obtained.

    In this study, MGF / PEG-MGF was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking MGF / PEG-MGF.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 21:44 UTC

    Study results updated

  2. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

Publication types

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