OR21-4 Comparison of the Effects of PTH (1-34), PTHrP (1-36) and Abaloparatide (ABL) on the Murine Osteoblast Transcriptome

The source lists no authors.

Journal of the Endocrine Society1 Nov 2022

PMCIDPMC9624605PepBankPUB-91285805

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Summary

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Text rights: CC BY-NC-ND. Results still quote short attributed sentences.

Results from this study7

4 results from studies that gave the peptide. No result from this study comes from people.

01LabIn vitro (cells)Mechanism
In mouse calvarial osteoblasts, abaloparatide increased VDR mRNA, with confirmation by qRT-PCR.
In plain wordsIn mouse cells, abaloparatide increased vitamin D receptor gene activity.
Peptide
Abaloparatide (Tymlos)
Outcome
IncreaseVDR mRNA
Study
mousen: not reported in source
Calvarial osteoblasts
Outcome group
Receptor activity and mechanism · Musculoskeletal
Studied dose
1 nM · 4 hAs given in this study (mouse).
Source sentences
Summary
Further analysis of the data illuminated that the three peptides increased Vitamin D receptor (VDR) and Cbp/p300-interacting transactivator 1 (CITED1) mRNAs similar to RankL expression.
2 context sentences(design, population, supporting numbers)
Summary · context
These findings were confirmed via qRT-PCR of additional cultured samples of mouse calvarial osteoblasts, treated with 1 nM of PTH (1-34), PTHrP (1-36), and ABL for 4 h prior to harvest.
Summary · context
In this study we show that treatment of mouse calvarial osteoblasts with 1 nM of these peptides for 4 h results in differing effects on the osteoblast transcriptome by performing gene enrichment analysis of RNA-Seq data.
Report a problemFND-9128580504
02LabIn vitro (cells)Mechanism
In mouse calvarial osteoblasts, abaloparatide increased CITED1 mRNA, with confirmation by qRT-PCR.
In plain wordsIn mouse cells, abaloparatide increased CITED1 gene activity.
Peptide
Abaloparatide (Tymlos)
Outcome
IncreaseCITED1 mRNA
Study
mousen: not reported in source
Calvarial osteoblasts
Outcome group
Receptor activity and mechanism · Musculoskeletal
Studied dose
1 nM · 4 hAs given in this study (mouse).
Source sentences
Summary
Further analysis of the data illuminated that the three peptides increased Vitamin D receptor (VDR) and Cbp/p300-interacting transactivator 1 (CITED1) mRNAs similar to RankL expression.
2 context sentences(design, population, supporting numbers)
Summary · context
These findings were confirmed via qRT-PCR of additional cultured samples of mouse calvarial osteoblasts, treated with 1 nM of PTH (1-34), PTHrP (1-36), and ABL for 4 h prior to harvest.
Summary · context
In this study we show that treatment of mouse calvarial osteoblasts with 1 nM of these peptides for 4 h results in differing effects on the osteoblast transcriptome by performing gene enrichment analysis of RNA-Seq data.
Report a problemFND-9128580505
03LabIn vitro (cells)Mechanism
In mouse calvarial osteoblasts, abaloparatide increased RankL mRNA, with confirmation by qRT-PCR.
In plain wordsIn mouse cells, abaloparatide increased RankL gene activity.
Peptide
Abaloparatide (Tymlos)
Outcome
IncreaseRankL mRNA
Study
mousen: not reported in source
Calvarial osteoblasts
Outcome group
Receptor activity and mechanism · Musculoskeletal
Studied dose
1 nM · 4 hAs given in this study (mouse).
Source sentences
Summary
Further analysis of the data illuminated that the three peptides increased Vitamin D receptor (VDR) and Cbp/p300-interacting transactivator 1 (CITED1) mRNAs similar to RankL expression.
2 context sentences(design, population, supporting numbers)
Summary · context
These findings were confirmed via qRT-PCR of additional cultured samples of mouse calvarial osteoblasts, treated with 1 nM of PTH (1-34), PTHrP (1-36), and ABL for 4 h prior to harvest.
Summary · context
In this study we show that treatment of mouse calvarial osteoblasts with 1 nM of these peptides for 4 h results in differing effects on the osteoblast transcriptome by performing gene enrichment analysis of RNA-Seq data.
Report a problemFND-9128580506
04LabIn vitro (cells)Mechanism
In mouse calvarial osteoblasts, abaloparatide significantly increased Wnt11 mRNA, with confirmation by qRT-PCR.
In plain wordsIn mouse cells, abaloparatide increased Wnt11 gene activity.
Peptide
Abaloparatide (Tymlos)
Outcome
IncreaseWnt11 mRNA
Study
mousen: not reported in source
Calvarial osteoblasts
Outcome group
Receptor activity and mechanism · Musculoskeletal
Studied dose
1 nM · 4 hAs given in this study (mouse).
Source sentences
Summary
Pathway analysis and subsequent qRT-PCR confirmation has shown that PTH (1-34) and ABL lead to a significant increase in Wnt11 mRNA, while PTHrP (1-36) does not.
1 context sentence(design, population, supporting numbers)
Summary · context
In this study we show that treatment of mouse calvarial osteoblasts with 1 nM of these peptides for 4 h results in differing effects on the osteoblast transcriptome by performing gene enrichment analysis of RNA-Seq data.
Report a problemFND-9128580507

Studies that did not test the peptide alone3

In this study, Abaloparatide (Tymlos) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Abaloparatide (Tymlos).

05Computer modelNot a test of Abaloparatide (Tymlos) aloneIn silicoMechanism
RNA sequencing analysis of treated mouse osteoblasts identified 179 abaloparatide-regulated genes, including 20 unique genes.
In plain wordsIn computer analyses of mouse cell data, abaloparatide regulated 179 genes, including 20 unique genes.
Peptide
Abaloparatide (Tymlos)
Outcome
Not applicableRegulated genes
Study
mousen: not reported in source
Calvarial osteoblasts
Outcome group
Receptor activity and mechanism · Musculoskeletal
Measurements
Regulated genes
179 genes
ABL
Unique regulated genes
20 genes
ABL
Source sentences
Summary
RNA-Sequencing revealed that PTH (1-34) regulated 367 genes, 194 were unique; PTHrP (1-36) regulated 117 genes, 15 were unique; ABL regulated 179 genes, 20 were unique.
1 context sentence(design, population, supporting numbers)
Summary · context
In this study we show that treatment of mouse calvarial osteoblasts with 1 nM of these peptides for 4 h results in differing effects on the osteoblast transcriptome by performing gene enrichment analysis of RNA-Seq data.
In this study, Abaloparatide (Tymlos) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Abaloparatide (Tymlos).
Report a problemFND-9128580501
06Computer modelNot a test of Abaloparatide (Tymlos) aloneIn silicoMechanism
Mouse osteoblast transcriptome analysis found 74 genes shared only by PTH and abaloparatide and 83 shared by all three peptides.
In plain wordsIn computer analyses of mouse cell data, abaloparatide shared regulated genes with the other tested peptides.
Peptide
Abaloparatide (Tymlos)
Outcome
Not applicableShared regulated genes
Study
mousen: not reported in source
Calvarial osteoblasts
Outcome group
Receptor activity and mechanism · Musculoskeletal
Source sentences
Summary
There were 74 genes shared only among PTH(1-34) and ABL; 16 genes shared only among PTH (1-34) and PTHrP; and 83 genes shared among all three peptides.
1 context sentence(design, population, supporting numbers)
Summary · context
In this study we show that treatment of mouse calvarial osteoblasts with 1 nM of these peptides for 4 h results in differing effects on the osteoblast transcriptome by performing gene enrichment analysis of RNA-Seq data.
In this study, Abaloparatide (Tymlos) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Abaloparatide (Tymlos).
Report a problemFND-9128580502
07Computer modelNot a test of Abaloparatide (Tymlos) aloneIn silicoMechanism
Mouse osteoblast transcriptome analysis identified pathway-specific differences among abaloparatide, PTH and PTHrP treatments.
In plain wordsIn computer analyses of mouse cell data, abaloparatide and the other peptides differed across several pathways.
Peptide
Abaloparatide (Tymlos)
Outcome
MixedPathway-specific transcriptome differences
Study
mousen: not reported in source
Calvarial osteoblasts
Outcome group
Receptor activity and mechanism · Musculoskeletal
Source sentences
Summary
Data collected show pathway-specific differences including, 1) cAMP/PKA, 2) Wnt/β-catenin, 3) Transcriptional regulation 4) Inflammatory response 5) Transmembrane transport, 6) Metabolism, and 7) NF-kB.
1 context sentence(design, population, supporting numbers)
Summary · context
In this study we show that treatment of mouse calvarial osteoblasts with 1 nM of these peptides for 4 h results in differing effects on the osteoblast transcriptome by performing gene enrichment analysis of RNA-Seq data.
In this study, Abaloparatide (Tymlos) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Abaloparatide (Tymlos).
Report a problemFND-9128580503

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 3Current
22 Oct 2026, 02:25 UTC
Study results updated
Version 2
21 Oct 2026, 18:59 UTC
Study data updated · source update from Europe PMC
Version 1
21 Oct 2026, 18:59 UTC
First added · source update from Europe PMC

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Abstract

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