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Study · In vitro (cells) · 2026

5 results

Augmented Skin Beneficial Effects of Thermus Thermophilus and Bacillus Subtilis Mixed-Culture Ferment Extract by Tripeptide GHK-Cu.

Wang J, Tao K, Huang H, Chang H

Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)1 Aug 2026

PMID42573538 (opens in a new tab)PMCIDPMC13455814 (opens in a new tab)DOI10.1111/srt.70360 (opens in a new tab)PepBankPUB-42573538
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: CC BY-NC-ND. Results still quote short attributed sentences.

Results from this study5

1 result from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Efficacy outcome

    In lipopolysaccharide-stimulated macrophages, the TBFE plus GHK‑Cu combination inhibited inflammatory cytokine secretion more than TBFE or GHK‑Cu alone.

    In plain wordsIn cells, the ferment extract plus GHK‑Cu lowered inflammatory signals more than either one alone.

    Peptide
    GHK-Cu
    Outcome
    DecreaseInflammatory cytokine secretion
    Study

    n: not reported in source

    LPS-stimulated macrophages
    Comparator
    TBFE alone or GHK-Cu alone
    Outcome group
    Inflammation markers · Immune and inflammation
    Studied dose
    Dose not stated

    Source sentences

    Summary
    The combination outperformed TBFE or GHK-Cu alone in inhibiting secretion of inflammatory cytokines by lipopolysaccharide-stimulated macrophages.
    1 context sentence
    Summary · context
    An extract of the Thermus thermophilus and Bacillus subtilis mixed-culture ferment (TBFE) was prepared and the skin protective effects of its combination with the human tripeptide derivative GHK-Cu were explored through in vitro, ex vivo, and clinical tests.

Studies that did not test the peptide alone4

In this study, GHK-Cu was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking GHK-Cu.

  1. 02HumanNot a test of GHK-Cu aloneOther designEfficacy outcome

    In a clinical study, the TBFE and GHK‑Cu combination was effective in decreasing wrinkles.

    In plain wordsIn a clinical study, a product with ferment extract and GHK‑Cu was linked to fewer wrinkles.

    Peptide
    GHK-Cu
    Outcome
    DecreaseWrinkles
    Study

    n: not reported in source

    Outcome group
    Skin and hair · Skin and wound

    Source sentences

    Summary
    Finally, results from the clinical study demonstrated that the combination was effective in decreasing wrinkles and improving skin barrier conditions.
    1 context sentence
    Summary · context
    An extract of the Thermus thermophilus and Bacillus subtilis mixed-culture ferment (TBFE) was prepared and the skin protective effects of its combination with the human tripeptide derivative GHK-Cu were explored through in vitro, ex vivo, and clinical tests.

    In this study, GHK-Cu was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHK-Cu.

  2. 03HumanNot a test of GHK-Cu aloneOther designEfficacy outcome

    In a clinical study, the TBFE and GHK‑Cu combination was effective in improving skin barrier conditions.

    In plain wordsIn a clinical study, a product with ferment extract and GHK‑Cu was linked to better skin barrier condition.

    Peptide
    GHK-Cu
    Outcome
    IncreaseSkin barrier condition
    Study

    n: not reported in source

    Outcome group
    Skin and hair · Skin and wound

    Source sentences

    Summary
    Finally, results from the clinical study demonstrated that the combination was effective in decreasing wrinkles and improving skin barrier conditions.
    1 context sentence
    Summary · context
    An extract of the Thermus thermophilus and Bacillus subtilis mixed-culture ferment (TBFE) was prepared and the skin protective effects of its combination with the human tripeptide derivative GHK-Cu were explored through in vitro, ex vivo, and clinical tests.

    In this study, GHK-Cu was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHK-Cu.

  3. 04LabNot a test of GHK-Cu aloneEx vivo tissueEfficacy outcome

    In human skin explants, a face cream with the TBFE and GHK‑Cu combination as sole active ingredients protected against UV damage by maintaining skin morphology.

    In plain wordsIn lab tests on human skin samples, a cream with the combination helped keep skin structure after UV exposure.

    Peptide
    GHK-Cu
    Outcome
    Not applicableSkin morphology after UV irradiation
    Study

    n: not reported in source

    Human skin explants with UV irradiation
    Outcome group
    Skin and hair · Skin and wound

    Source sentences

    Summary
    In addition, one face cream formulated with the combination as the sole active ingredients protected human skin explants from ultraviolet irradiation induced damage by maintaining skin morphology and decreasing the degradation of extracellular matrix.
    1 context sentence
    Summary · context
    An extract of the Thermus thermophilus and Bacillus subtilis mixed-culture ferment (TBFE) was prepared and the skin protective effects of its combination with the human tripeptide derivative GHK-Cu were explored through in vitro, ex vivo, and clinical tests.

    In this study, GHK-Cu was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHK-Cu.

  4. 05LabNot a test of GHK-Cu aloneEx vivo tissueEfficacy outcome

    In human skin explants, a face cream with the TBFE and GHK‑Cu combination decreased UV-induced degradation of extracellular matrix.

    In plain wordsIn lab tests on human skin samples, a cream with the combination reduced UV-linked breakdown of skin support structure.

    Peptide
    GHK-Cu
    Outcome
    DecreaseExtracellular matrix degradation after UV irradiation
    Study

    n: not reported in source

    Human skin explants with UV irradiation
    Outcome group
    Skin and hair · Skin and wound

    Source sentences

    Summary
    In addition, one face cream formulated with the combination as the sole active ingredients protected human skin explants from ultraviolet irradiation induced damage by maintaining skin morphology and decreasing the degradation of extracellular matrix.
    1 context sentence
    Summary · context
    An extract of the Thermus thermophilus and Bacillus subtilis mixed-culture ferment (TBFE) was prepared and the skin protective effects of its combination with the human tripeptide derivative GHK-Cu were explored through in vitro, ex vivo, and clinical tests.

    In this study, GHK-Cu was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHK-Cu.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 3Current

    9 Oct 2026, 18:17 UTC

    Study results updated

  2. Version 2

    9 Oct 2026, 03:03 UTC

    Study data updated · source update from Europe PMC

  3. Version 1

    9 Oct 2026, 02:56 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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