A discovery-based proteomic approach of epidermal growth factor and growth hormone-releasing peptide-6 in a model of acute ischemic stroke.

Rodríguez-Ulloa A, Subirós-Martínez N, González LJ, Risco-Acevedo D, Guillén-Nieto GE, Besada V, García-Del-Barco-Herrera D

Pharmacological reports : PR8 Jun 2026

PMID42258095DOI10.1007/s43440-026-00872-4PepBankPUB-42258095

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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study7

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone7

In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking GHRP-6.

01AnimalNot a test of GHRP-6 aloneAnimal study (in vivo)Mechanism
In ischemic rats, EGF+GHRP6 differentially modulated 40 proteins at 3 h versus vehicle.
In plain wordsIn rats with brain ischemia, EGF+GHRP6 changed 40 proteins at 3 h versus vehicle.
Peptide
GHRP-6
Outcome
MixedDifferentially modulated proteins at 3 h
Study
ratn: not reported in source
Male Wistar rats with ET-1-induced MCA occlusion
Comparator
Vehicle-treated ischemic rats
Outcome group
Neuroprotection · Nervous system and cognition
Measurements
Differentially modulated proteins
40 proteins
3 h
Source sentences
Summary
Proteomic profiling validated the ischemic model and revealed 40 and 223 proteins differentially modulated by EGF+GHRP6 at 3 and 24 h, respectively.
2 context sentences(design, population, supporting numbers)
Summary · context
Male Wistar rats were subjected to endothelin-1 (ET-1)-induced middle cerebral artery (MCA) occlusion and randomly assigned to three experimental groups: EGF+GHRP6‑treated ischemic, vehicle‑treated ischemic, and sham-operated controls (n = 12 per group).
Summary · context
Label-free quantitative proteomic analysis of the ischemic penumbra was performed at 3 and 24 h post-treatment.
In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.
Report a problemFND-4225809501
02AnimalNot a test of GHRP-6 aloneAnimal study (in vivo)Mechanism
In ischemic rats, EGF+GHRP6 differentially modulated 223 proteins at 24 h versus vehicle.
In plain wordsIn rats with brain ischemia, EGF+GHRP6 changed 223 proteins at 24 h versus vehicle.
Peptide
GHRP-6
Outcome
MixedDifferentially modulated proteins at 24 h
Study
ratn: not reported in source
Male Wistar rats with ET-1-induced MCA occlusion
Comparator
Vehicle-treated ischemic rats
Outcome group
Neuroprotection · Nervous system and cognition
Measurements
Differentially modulated proteins
223 proteins
24 h
Source sentences
Summary
Proteomic profiling validated the ischemic model and revealed 40 and 223 proteins differentially modulated by EGF+GHRP6 at 3 and 24 h, respectively.
2 context sentences(design, population, supporting numbers)
Summary · context
Male Wistar rats were subjected to endothelin-1 (ET-1)-induced middle cerebral artery (MCA) occlusion and randomly assigned to three experimental groups: EGF+GHRP6‑treated ischemic, vehicle‑treated ischemic, and sham-operated controls (n = 12 per group).
Summary · context
Label-free quantitative proteomic analysis of the ischemic penumbra was performed at 3 and 24 h post-treatment.
In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.
Report a problemFND-4225809502
03AnimalNot a test of GHRP-6 aloneAnimal study (in vivo)Mechanism
In ischemic rats, EGF+GHRP6 modulated anti-apoptotic proteins in the ischemic penumbra.
In plain wordsIn rats with brain ischemia, EGF+GHRP6 changed proteins that oppose cell death in the ischemic penumbra.
Peptide
GHRP-6
Outcome
Direction not reportedAnti-apoptotic proteins
Study
ratn: not reported in source
Male Wistar rats with ET-1-induced MCA occlusion
Comparator
Vehicle-treated ischemic rats
Outcome group
Neuroprotection · Nervous system and cognition
Source sentences
Summary
Additionally, anti-apoptotic and mitochondrial proteins were modulated in the ischemic penumbra, supporting the neuroprotective effects of EGF+GHRP6.
2 context sentences(design, population, supporting numbers)
Summary · context
Male Wistar rats were subjected to endothelin-1 (ET-1)-induced middle cerebral artery (MCA) occlusion and randomly assigned to three experimental groups: EGF+GHRP6‑treated ischemic, vehicle‑treated ischemic, and sham-operated controls (n = 12 per group).
Summary · context
Label-free quantitative proteomic analysis of the ischemic penumbra was performed at 3 and 24 h post-treatment.
In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.
Report a problemFND-4225809504
04AnimalNot a test of GHRP-6 aloneAnimal study (in vivo)Mechanism
In ischemic rats, EGF+GHRP6 modulated mitochondrial proteins in the ischemic penumbra.
In plain wordsIn rats with brain ischemia, EGF+GHRP6 changed mitochondrial proteins in the ischemic penumbra.
Peptide
GHRP-6
Outcome
Direction not reportedMitochondrial proteins
Study
ratn: not reported in source
Male Wistar rats with ET-1-induced MCA occlusion
Comparator
Vehicle-treated ischemic rats
Outcome group
Neuroprotection · Nervous system and cognition
Source sentences
Summary
Additionally, anti-apoptotic and mitochondrial proteins were modulated in the ischemic penumbra, supporting the neuroprotective effects of EGF+GHRP6.
2 context sentences(design, population, supporting numbers)
Summary · context
Male Wistar rats were subjected to endothelin-1 (ET-1)-induced middle cerebral artery (MCA) occlusion and randomly assigned to three experimental groups: EGF+GHRP6‑treated ischemic, vehicle‑treated ischemic, and sham-operated controls (n = 12 per group).
Summary · context
Label-free quantitative proteomic analysis of the ischemic penumbra was performed at 3 and 24 h post-treatment.
In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.
Report a problemFND-4225809505
05AnimalNot a test of GHRP-6 aloneAnimal study (in vivo)Mechanism
In ischemic rats, EGF+GHRP6 upregulated proteins known to attenuate brain damage after stroke.
In plain wordsIn rats with brain ischemia, EGF+GHRP6 increased proteins known to limit stroke damage.
Peptide
GHRP-6
Outcome
IncreaseProteins that attenuate stroke damage
Study
ratn: not reported in source
Male Wistar rats with ET-1-induced MCA occlusion
Comparator
Vehicle-treated ischemic rats
Outcome group
Neuroprotection · Nervous system and cognition
Source sentences
Summary
Notably, proteins known to attenuate brain damage after stroke were up-regulated, while those promoting ischemic injury were down-regulated following the combined treatment.
2 context sentences(design, population, supporting numbers)
Summary · context
Male Wistar rats were subjected to endothelin-1 (ET-1)-induced middle cerebral artery (MCA) occlusion and randomly assigned to three experimental groups: EGF+GHRP6‑treated ischemic, vehicle‑treated ischemic, and sham-operated controls (n = 12 per group).
Summary · context
Label-free quantitative proteomic analysis of the ischemic penumbra was performed at 3 and 24 h post-treatment.
In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.
Report a problemFND-4225809506
06AnimalNot a test of GHRP-6 aloneAnimal study (in vivo)Mechanism
In ischemic rats, EGF+GHRP6 downregulated proteins that promote ischemic injury.
In plain wordsIn rats with brain ischemia, EGF+GHRP6 reduced proteins that promote ischemic injury.
Peptide
GHRP-6
Outcome
DecreaseProteins that promote ischemic injury
Study
ratn: not reported in source
Male Wistar rats with ET-1-induced MCA occlusion
Comparator
Vehicle-treated ischemic rats
Outcome group
Neuroprotection · Nervous system and cognition
Source sentences
Summary
Notably, proteins known to attenuate brain damage after stroke were up-regulated, while those promoting ischemic injury were down-regulated following the combined treatment.
2 context sentences(design, population, supporting numbers)
Summary · context
Male Wistar rats were subjected to endothelin-1 (ET-1)-induced middle cerebral artery (MCA) occlusion and randomly assigned to three experimental groups: EGF+GHRP6‑treated ischemic, vehicle‑treated ischemic, and sham-operated controls (n = 12 per group).
Summary · context
Label-free quantitative proteomic analysis of the ischemic penumbra was performed at 3 and 24 h post-treatment.
In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.
Report a problemFND-4225809507
07Computer modelNot a test of GHRP-6 aloneIn silicoMechanism
In ischemic rats, neurotransmitter transport proteins were overrepresented in the EGF+GHRP6-regulated proteome at both time points.
In plain wordsIn rats with brain ischemia, neurotransmitter transport proteins were overrepresented among proteins regulated by EGF+GHRP6.
Peptide
GHRP-6
Outcome
IncreaseNeurotransmitter transport protein enrichment
Study
n: not applicable
Bioinformatic functional enrichment and pathway analysis of rat ischemic penumbra proteomic data
Comparator
Vehicle-treated ischemic rats
Outcome group
Neuroprotection · Nervous system and cognition
Source sentences
Summary
Proteins involved in neurotransmitter transport were consistently overrepresented in the EGF+GHRP6-regulated proteome at both time points.
3 context sentences(design, population, supporting numbers)
Summary · context
Functional enrichment and pathway analysis of differentially modulated proteins were performed using bioinformatics tools.
Summary · context
Male Wistar rats were subjected to endothelin-1 (ET-1)-induced middle cerebral artery (MCA) occlusion and randomly assigned to three experimental groups: EGF+GHRP6‑treated ischemic, vehicle‑treated ischemic, and sham-operated controls (n = 12 per group).
Summary · context
Label-free quantitative proteomic analysis of the ischemic penumbra was performed at 3 and 24 h post-treatment.
In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.
Report a problemFND-4225809503

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
21 Oct 2026, 15:58 UTC
Study results updated
Version 1
9 Oct 2026, 03:13 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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Keywords

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