Exosomes Released by Cerebrolysin-Treated Cerebral Endothelial Cells Reverse Fibrin- or tPA-Impaired Endothelial Cell Permeability.

Teng H, Li C, Wang M, Zhang J, Zhang Y, Chopp M, Zhang ZG

Cells19 May 2026

PMID42193943PMCIDPMC13205112DOI10.3390/cells15100934PepBankPUB-42193943

Summary

Cerebrolysin has a salutary effect on impaired cerebral endothelial cell (CEC) permeability. Using an in vitro endothelial permeability assay, the present study tested the hypothesis that exosomes released by Cerebrolysin-treated CECs (Cerebro-Exos) have a robust therapeutic effect on dysfunctional CECs. Stoichiometric analysis showed marked differences in cargo profiles between Cerebro-Exos and exosomes derived from CECs without Cerebrolysin treatment (Naïve-Exos), in which Cerebro-Exos were highly enriched with metabolic and tight junction related proteins compared to Naïve-Exos. Cerebro-Exos had a superior effect compared to Naïve-Exos on restoring CEC integrity impaired by fibrin and tissue plasminogen activator (tPA). Treatment of fibrin- and tPA-challenged CECs with Cerebro-Exos robustly reduced fibrin- and tPA-augmented proteins involved in inflammation and coagulation and substantially increased fibrin- and tPA-decreased proteins that are related to tight junctions and metabolism. Collectively, these data indicate that Cerebro-Exos have a broad effect on improvement of dysfunctional CECs, which is likely achieved by the alteration of CEC proteins.

CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study7

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone7

In this study, Cerebrolysin was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Cerebrolysin.

01LabNot a test of Cerebrolysin aloneIn vitro (cells)Mechanism
In lab tests, exosomes from Cerebrolysin-treated cells contained more metabolic proteins than Naïve-Exos.
In plain wordsIn lab tests, exosomes from Cerebrolysin-treated cells contained more metabolic proteins than Naïve-Exos.
Peptide
Cerebrolysin
Outcome
IncreaseMetabolic protein cargo
Study
n: not reported in source
Cerebral endothelial cells
Comparator
Naïve-Exos
Outcome group
Receptor activity and mechanism · Cardiovascular
Source sentences
Summary
Stoichiometric analysis showed marked differences in cargo profiles between Cerebro-Exos and exosomes derived from CECs without Cerebrolysin treatment (Naïve-Exos), in which Cerebro-Exos were highly enriched with metabolic and tight junction related proteins compared to Naïve-Exos.
1 context sentence(design, population, supporting numbers)
Summary · context
Using an in vitro endothelial permeability assay, the present study tested the hypothesis that exosomes released by Cerebrolysin-treated CECs (Cerebro-Exos) have a robust therapeutic effect on dysfunctional CECs.
In this study, Cerebrolysin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Cerebrolysin.
Show in the study text Report a problemFND-4219394301
02LabNot a test of Cerebrolysin aloneIn vitro (cells)Mechanism
In lab tests, exosomes from Cerebrolysin-treated cells contained more tight junction proteins than Naïve-Exos.
In plain wordsIn lab tests, exosomes from Cerebrolysin-treated cells contained more tight junction proteins than Naïve-Exos.
Peptide
Cerebrolysin
Outcome
IncreaseTight junction protein cargo
Study
n: not reported in source
Cerebral endothelial cells
Comparator
Naïve-Exos
Outcome group
Receptor activity and mechanism · Cardiovascular
Source sentences
Summary
Stoichiometric analysis showed marked differences in cargo profiles between Cerebro-Exos and exosomes derived from CECs without Cerebrolysin treatment (Naïve-Exos), in which Cerebro-Exos were highly enriched with metabolic and tight junction related proteins compared to Naïve-Exos.
1 context sentence(design, population, supporting numbers)
Summary · context
Using an in vitro endothelial permeability assay, the present study tested the hypothesis that exosomes released by Cerebrolysin-treated CECs (Cerebro-Exos) have a robust therapeutic effect on dysfunctional CECs.
In this study, Cerebrolysin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Cerebrolysin.
Show in the study text Report a problemFND-4219394302
03LabNot a test of Cerebrolysin aloneIn vitro (cells)Efficacy outcome
In cells challenged with fibrin and tPA, Cerebro-Exos restored integrity more effectively than Naïve-Exos.
In plain wordsIn cells challenged with fibrin and tPA, Cerebro-Exos restored integrity more effectively than Naïve-Exos.
Peptide
Cerebrolysin
Outcome
IncreaseEndothelial integrity
Study
n: not reported in source
Cerebral endothelial cells
Comparator
Naïve-Exos
Outcome group
Receptor activity and mechanism · Cardiovascular
Source sentences
Summary
Cerebro-Exos had a superior effect compared to Naïve-Exos on restoring CEC integrity impaired by fibrin and tissue plasminogen activator (tPA).
1 context sentence(design, population, supporting numbers)
Summary · context
Using an in vitro endothelial permeability assay, the present study tested the hypothesis that exosomes released by Cerebrolysin-treated CECs (Cerebro-Exos) have a robust therapeutic effect on dysfunctional CECs.
In this study, Cerebrolysin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Cerebrolysin.
Show in the study text Report a problemFND-4219394303
04LabNot a test of Cerebrolysin aloneIn vitro (cells)Mechanism
In cells challenged with fibrin and tPA, Cerebro-Exos reduced augmented inflammation-related proteins.
In plain wordsIn cells challenged with fibrin and tPA, Cerebro-Exos reduced augmented inflammation-related proteins.
Peptide
Cerebrolysin
Outcome
DecreaseInflammation-related proteins
Study
n: not reported in source
Cerebral endothelial cells
Outcome group
Inflammation markers · Cardiovascular
Source sentences
Summary
Treatment of fibrin- and tPA-challenged CECs with Cerebro-Exos robustly reduced fibrin- and tPA-augmented proteins involved in inflammation and coagulation and substantially increased fibrin- and tPA-decreased proteins that are related to tight junctions and metabolism.
1 context sentence(design, population, supporting numbers)
Summary · context
Using an in vitro endothelial permeability assay, the present study tested the hypothesis that exosomes released by Cerebrolysin-treated CECs (Cerebro-Exos) have a robust therapeutic effect on dysfunctional CECs.
In this study, Cerebrolysin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Cerebrolysin.
Show in the study text Report a problemFND-4219394304
05LabNot a test of Cerebrolysin aloneIn vitro (cells)Mechanism
In cells challenged with fibrin and tPA, Cerebro-Exos reduced augmented coagulation-related proteins.
In plain wordsIn cells challenged with fibrin and tPA, Cerebro-Exos reduced augmented coagulation-related proteins.
Peptide
Cerebrolysin
Outcome
DecreaseCoagulation-related proteins
Study
n: not reported in source
Cerebral endothelial cells
Outcome group
Receptor activity and mechanism · Cardiovascular
Source sentences
Summary
Treatment of fibrin- and tPA-challenged CECs with Cerebro-Exos robustly reduced fibrin- and tPA-augmented proteins involved in inflammation and coagulation and substantially increased fibrin- and tPA-decreased proteins that are related to tight junctions and metabolism.
1 context sentence(design, population, supporting numbers)
Summary · context
Using an in vitro endothelial permeability assay, the present study tested the hypothesis that exosomes released by Cerebrolysin-treated CECs (Cerebro-Exos) have a robust therapeutic effect on dysfunctional CECs.
In this study, Cerebrolysin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Cerebrolysin.
Show in the study text Report a problemFND-4219394305
06LabNot a test of Cerebrolysin aloneIn vitro (cells)Mechanism
In cells challenged with fibrin and tPA, Cerebro-Exos increased depleted tight junction-related proteins.
In plain wordsIn cells challenged with fibrin and tPA, Cerebro-Exos increased depleted tight junction-related proteins.
Peptide
Cerebrolysin
Outcome
IncreaseTight junction-related proteins
Study
n: not reported in source
Cerebral endothelial cells
Outcome group
Receptor activity and mechanism · Cardiovascular
Source sentences
Summary
Treatment of fibrin- and tPA-challenged CECs with Cerebro-Exos robustly reduced fibrin- and tPA-augmented proteins involved in inflammation and coagulation and substantially increased fibrin- and tPA-decreased proteins that are related to tight junctions and metabolism.
1 context sentence(design, population, supporting numbers)
Summary · context
Using an in vitro endothelial permeability assay, the present study tested the hypothesis that exosomes released by Cerebrolysin-treated CECs (Cerebro-Exos) have a robust therapeutic effect on dysfunctional CECs.
In this study, Cerebrolysin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Cerebrolysin.
Show in the study text Report a problemFND-4219394306
07LabNot a test of Cerebrolysin aloneIn vitro (cells)Mechanism
In cells challenged with fibrin and tPA, Cerebro-Exos increased depleted metabolism-related proteins.
In plain wordsIn cells challenged with fibrin and tPA, Cerebro-Exos increased depleted metabolism-related proteins.
Peptide
Cerebrolysin
Outcome
IncreaseMetabolism-related proteins
Study
n: not reported in source
Cerebral endothelial cells
Outcome group
Receptor activity and mechanism · Cardiovascular
Source sentences
Summary
Treatment of fibrin- and tPA-challenged CECs with Cerebro-Exos robustly reduced fibrin- and tPA-augmented proteins involved in inflammation and coagulation and substantially increased fibrin- and tPA-decreased proteins that are related to tight junctions and metabolism.
1 context sentence(design, population, supporting numbers)
Summary · context
Using an in vitro endothelial permeability assay, the present study tested the hypothesis that exosomes released by Cerebrolysin-treated CECs (Cerebro-Exos) have a robust therapeutic effect on dysfunctional CECs.
In this study, Cerebrolysin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Cerebrolysin.
Show in the study text Report a problemFND-4219394307

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
14 Oct 2026, 05:02 UTC
Study results updated
Version 1
14 Oct 2026, 01:46 UTC
First added · source update from Europe PMC

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