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Study · Not a test of the peptide alone · 2026

4 results

The Laccase-like Property of GHK-Cu and Its Applications in Colorimetric Sensing of Phenolic Compounds.

Chen JS, Zhu H, Chai TQ, Yang FQ

Biosensors12 Apr 2026Cited by 1 (Europe PMC)

PMID42041438 (opens in a new tab)PMCIDPMC13115362 (opens in a new tab)DOI10.3390/bios16040217 (opens in a new tab)PepBankPUB-42041438
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Summary

Laccase plays an important role in the detection and degradation of phenolic compounds, but it is limited by its cost and stability. In this study, the laccase-like property of copper peptide (GHK-Cu) has been revealed. In terms of enzymatic reaction kinetics, GHK-Cu has a Vmax of 1.735 × 10-4 mM·s-1 and a Km of 0.061 mM, demonstrating good substrate affinity and excellent catalytic efficiency. Then, a colorimetry was developed for rapid detection of epinephrine (EP) and 2-aminophenol (2-AP). The linear response range of EP is 20-240 μM, with a limit of detection (LOD) of 9.5 μM. The linear response ranges of 2-AP are 14-100 μM (in ultrapure water) and 2-120 μM (in seawater), with LODs of 2.56 μM and 1.65 μM. In addition, combined with a smartphone platform, a cotton-based sensor has been developed for the detection of 2-AP in seawater. The linear response ranges are 0-0.2 mM and 0.2-1 mM, with LOD of 0.033 mM. The structure of GHK-Cu provides a reference for the development of novel laccase mimetic enzymes. The constructed colorimetry offers an option for the rapid detection of phenolic compounds, and the developed cotton-based sensor enabled rapid and portable detection of 2-AP.
CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study4

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone4

In this study, GHK-Cu was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking GHK-Cu.

  1. 01LabNot a test of GHK-Cu aloneIn vitro (cell-free)Activity measurement

    In cell-free assays, GHK‑Cu showed laccase-like activity with a Vmax of 1.735 x 10^-4 mM/s and a Km of 0.061 mM.

    In plain wordsIn lab tests, GHK‑Cu acted like the enzyme laccase and showed good substrate binding.

    Peptide
    GHK-Cu
    Outcome
    Not applicableLaccase-like enzyme kinetics
    Study

    n: not reported in source

    Outcome group
    Receptor activity and mechanism · Pharmacology

    Measurements

    Vmax
    1.735 x10^-4 mM·s^-1
    GHK-Cu laccase-like activity
    Km
    0.061 mM
    GHK-Cu laccase-like activity

    Source sentences

    Summary
    In terms of enzymatic reaction kinetics, GHK-Cu has a Vmax of 1.735 × 10-4 mM·s-1 and a Km of 0.061 mM, demonstrating good substrate affinity and excellent catalytic efficiency.
    1 context sentence
    Summary · context
    In this study, the laccase-like property of copper peptide (GHK-Cu) has been revealed.

    In this study, GHK-Cu was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHK-Cu.

  2. 02LabNot a test of GHK-Cu aloneIn vitro (cell-free)Other

    A GHK‑Cu laccase-like colorimetric assay detected epinephrine with a linear range of 20-240 uM and a limit of detection of 9.5 uM.

    In plain wordsIn lab tests, a GHK‑Cu-based colour test detected epinephrine at low micromolar levels.

    Peptide
    GHK-Cu
    Outcome
    Not applicableColorimetric detection of epinephrine
    Study

    n: not reported in source

    Outcome group
    Analytical methods and detection · Pharmacology

    Measurements

    Linear range
    20 to 240 μM
    epinephrine
    LOD
    9.5 μM
    epinephrine

    Source sentences

    Summary
    The linear response range of EP is 20-240 μM, with a limit of detection (LOD) of 9.5 μM.
    1 context sentence
    Summary · context
    In this study, the laccase-like property of copper peptide (GHK-Cu) has been revealed.

    In this study, GHK-Cu was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHK-Cu.

  3. 03LabNot a test of GHK-Cu aloneIn vitro (cell-free)Other

    A GHK‑Cu laccase-like colorimetric assay detected 2-aminophenol with linear ranges of 14-100 uM in ultrapure water and 2-120 uM in seawater.

    In plain wordsIn lab tests, a GHK‑Cu-based colour test detected 2-aminophenol in pure water and in seawater.

    Peptide
    GHK-Cu
    Outcome
    Not applicableColorimetric detection of 2-aminophenol
    Study

    n: not reported in source

    Outcome group
    Analytical methods and detection · Pharmacology

    Measurements

    Linear range
    14 to 100 μM
    2-AP in ultrapure water
    Linear range
    2 to 120 μM
    2-AP in seawater
    LOD
    2.56 μM
    2-AP in ultrapure water
    LOD
    1.65 μM
    2-AP in seawater

    Source sentences

    Summary
    The linear response ranges of 2-AP are 14-100 μM (in ultrapure water) and 2-120 μM (in seawater), with LODs of 2.56 μM and 1.65 μM.
    1 context sentence
    Summary · context
    In this study, the laccase-like property of copper peptide (GHK-Cu) has been revealed.

    In this study, GHK-Cu was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHK-Cu.

  4. 04LabNot a test of GHK-Cu aloneIn vitro (cell-free)Other

    A cotton-based sensor with a smartphone platform detected 2-aminophenol in seawater with linear ranges of 0-0.2 mM and 0.2-1 mM and a limit of detection of 0.033 mM.

    In plain wordsIn lab tests, a cotton sensor read by a smartphone detected 2-aminophenol in seawater.

    Peptide
    GHK-Cu
    Outcome
    Not applicableCotton-based smartphone sensor for 2-aminophenol in seawater
    Study

    n: not reported in source

    Outcome group
    Analytical methods and detection · Pharmacology

    Measurements

    Linear range
    0 to 0.2 mM
    2-AP in seawater, cotton sensor
    Linear range
    0.2 to 1 mM
    2-AP in seawater, cotton sensor
    LOD
    0.033 mM
    2-AP in seawater, cotton sensor

    Source sentences

    Summary
    In addition, combined with a smartphone platform, a cotton-based sensor has been developed for the detection of 2-AP in seawater. The linear response ranges are 0-0.2 mM and 0.2-1 mM, with LOD of 0.033 mM.
    1 context sentence
    Summary · context
    In this study, the laccase-like property of copper peptide (GHK-Cu) has been revealed.

    In this study, GHK-Cu was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHK-Cu.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 3Current

    9 Oct 2026, 18:17 UTC

    Study results updated

  2. Version 2

    9 Oct 2026, 03:03 UTC

    Study data updated · source update from Europe PMC

  3. Version 1

    9 Oct 2026, 02:56 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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